Unlock instant, AI-driven research and patent intelligence for your innovation.

Efficient fermentation medium and fermentation culture method for enterococcus faecium

A fermentation medium, Enterococcus faecium technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of low fermentation level, high price, increased cost, etc., to achieve high-efficiency fermentation, cost saving, The effect of increasing the amount of viable fermentation bacteria

Active Publication Date: 2018-10-26
QINGDAO VLAND BIOTECH INC +1
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, MRS medium is used for fermentation and cultivation of lactic acid bacteria species. However, MRS medium is expensive, and the number of viable bacteria after fermentation is lower than 1×10 9 CFU / mL, under the condition that the effective dosage of probiotics is fixed, the low fermentation level equals the increased cost in disguise

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Efficient fermentation medium and fermentation culture method for enterococcus faecium
  • Efficient fermentation medium and fermentation culture method for enterococcus faecium
  • Efficient fermentation medium and fermentation culture method for enterococcus faecium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] This embodiment provides the activation method of Enterococcus faecium E.F-2CCTCC No:M 2018314 strain (preserved in China Center for Type Culture Collection on May 28, 2018, and the preservation place is China. Wuhan. Wuhan University), details as follows:

[0028] Dilute the preserved bacteria powder of Enterococcus faecium with normal saline, mark three zones on the MRS plate medium, and place it in a constant temperature incubator at 37°C for 24 hours; pick a single colony on the plate medium and inoculate it in a container containing 100 mL of liquid MRS Place a 250mL Erlenmeyer flask with liquid culture medium in a constant temperature shaker at 37°C and incubate at 120r / min for 12 hours. Finally, use this bacterial solution to line the third section of the MRS plate medium and cultivate a new single colony for use.

Embodiment 2

[0030] This embodiment provides the growth curve determination method of Enterococcus faecium E.F-2CCTCC No:M 2018314, specifically as follows:

[0031] Pick a single colony of Enterococcus faecium and inoculate it into a 250mL Erlenmeyer flask containing 100mL of liquid MRS medium, place it on a constant temperature shaker at 37°C, and cultivate it at 120r / min for 12h, and then inoculate it with 1% (v / v) inoculum Inoculate into three bottles of 250mL Erlenmeyer flasks containing 150mL MRS liquid medium (30% liquid volume), culture in a constant temperature shaker at 37°C and 120r / min, and measure the bacterial liquid in the Erlenmeyer flasks with a turbidimeter every 2h Turbidity, see Table 1 for turbidity values, draw the growth curve of Enterococcus faecium E.F-2CCTCC No:M 2018314, such as figure 1 shown.

[0032] Table 1 Turbidity value of Enterococcus faecium CCTCC No:M 2018314

[0033]

[0034]

[0035] From Table 1 and attached figure 1 It can be seen that 0-2h...

Embodiment 3

[0037] This example provides the basis for selecting the most suitable carbon source in the culture medium of Enterococcus faecium E.F-2CCTCC No:M 2018314, specifically as follows:

[0038]Using different carbon sources instead of the carbon source in the MRS liquid medium to conduct a single factor test to screen the most suitable carbon source for the growth of Enterococcus faecium. Alternative carbon sources include: glucose, fructose, maltose, sucrose, lactose, soluble starch, corn flour, isomaltose, maltodextrin. The addition amount of different carbon sources was converted and added according to the principle of consistent carbon content in glucose in MRS liquid medium, and MRS liquid medium was set as the control group. The culture medium adjusted the same pH value of the MRS liquid medium, cultured at 120r / min at 37°C for 12h, and then measured the turbidity and the number of viable bacteria of Enterococcus faecium in different carbon source medium, with 10 -5 , 10 -...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an efficient fermentation medium and a fermentation culture method for enterococcus faecium, and belongs to the technical field of lactobacilli cultivation. The efficient fermentation medium has the advantages that fermentation processes can be optimized efficiently, the amount of fermented viable bacteria can be increased remarkably, and the cost is extremely low. A formulaof the efficient fermentation medium comprises 30-50g / L of corn flour, 3-6g / L of urea, 7-10g / L of crystalized sodium acetate, 2-4g / L of dipotassium phosphate, 0.05-0.40g / L of anhydrous magnesium sulfate, 0.04-0.08g / L of manganese sulfate, 1-3g / L of ammonium citrate dibasic and 0.8-1.2g / L of Tween-80. The efficient fermentation medium can be applied to efficient fermentation culture of enterococcus faecium E.F-2 with the CCTCC No:M 2018314.

Description

technical field [0001] The invention belongs to the technical field of lactic acid bacteria cultivation, and in particular relates to an ultra-low-cost and high-efficiency fermentation medium for Enterococcus faecium and a fermentation cultivation method thereof. Background technique [0002] At present, the irrational use of antibacterial drugs increases drug residues in livestock and poultry products, and drug-resistant strains also increase. Antibacterial drugs not only have the killing effect on harmful bacteria, but also inhibit and kill the normal microbial flora in the intestinal tract. Long-term use will lead to the imbalance of the normal flora in the gastrointestinal tract, causing endogenous infection or superinfection of animals. If things go on like this, it will seriously affect the sustainable development of the aquaculture industry, and it is also a huge threat to human food safety and the utilization of antimicrobial resources. Therefore, probiotics emerged...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 马丰英蒋贻海王艳玲王宏华崔栩孙亚磊刘元元魏波武利利
Owner QINGDAO VLAND BIOTECH INC
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com