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Preparation method of high-strength hydrogel serving as cartilage substitute and capable of combining with subchondral bone

A subchondral bone and hydrogel technology, which is applied in drug delivery, pharmaceutical formulation, medical science, etc., can solve the problems of poor mechanical properties of hydrogels, difficult to fix hydrogels in joints, etc., and achieve good biocompatibility , the preparation method is simple

Active Publication Date: 2018-10-30
TIANJIN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the deficiencies of the prior art, the technical problem to be solved by the present invention is that conventional hydrogels have poor mechanical properties, and it is difficult to fix hydrogels as articular cartilage substitutes on joints.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] a) Weigh 0.005g of sodium silicate, 5g of acrylamide, 0.5g of sodium alginate, and N,N'-methylenebisacrylamide with a mass percentage of 0.03% of acrylamide, and dissolve them in 50ml of deionized water together, stir and dissolve evenly , to obtain the casting solution A, and then dissolving trisodium phosphate in the casting solution A to obtain the casting solution B, and placing the casting solution A and the casting solution B in sterile containers for subsequent use after standing for defoaming;

[0022] b) preparing an aqueous calcium nitrate solution with a mass percentage of 0.5%;

[0023]c) Add respectively 0.1% ammonium persulfate of acrylamide mass percentage, 0.1% of acrylamide mass percentage of sodium bisulfite and 0.01% of acrylamide mass percentage to the casting solution A and casting film B prepared in step a). Tetramethylethylenediamine, after stirring and dispersing evenly, immediately pour the casting liquid B into a dry and clean glass plate, scra...

Embodiment 2

[0028] a) Weigh 2g of sodium silicate, 15g of acrylamide, 2g of sodium alginate, and N,N′-methylenebisacrylamide with a mass percentage of 0.30% of acrylamide, dissolve them in 100ml of deionized water, stir and dissolve evenly, and obtain Casting solution A, and then dissolving sodium dihydrogen phosphate in casting solution A to obtain casting solution B, placing casting solution A and casting solution B in sterile containers for subsequent use after defoaming;

[0029] B) preparation mass percentage is the calcium chloride aqueous solution of 50%;

[0030] c) Add respectively 5% ammonium persulfate of acrylamide mass percentage, 5% sodium bisulfite of acrylamide mass percentage and 2% acrylamide mass percentage to the casting solution A and casting film B prepared in step a). Tetramethylethylenediamine, after stirring and dispersing evenly, immediately pour the casting liquid B into a dry and clean glass plate, scrape a liquid film with a thickness of 1000 μm into a liquid ...

Embodiment 3

[0035] a) Weigh 1g of sodium silicate, 10g of acrylamide, 1g of sodium alginate, and N,N'-methylenebisacrylamide with a mass percentage of 0.10% of acrylamide, and dissolve them in 60ml of deionized water together, stir and dissolve evenly to obtain Casting solution A, and then potassium dihydrogen phosphate is dissolved in casting solution A to obtain casting solution B, and casting solution A and casting solution B are left to defoam and placed in sterile containers respectively for subsequent use;

[0036] b) preparing an aqueous calcium dihydrogen phosphate solution with a mass percentage of 2.0%;

[0037] c) Add respectively 1% ammonium persulfate of acrylamide mass percentage, 1% sodium bisulfite of acrylamide mass percentage and 1% acrylamide mass percentage to the casting solution A and casting film B prepared in step a). Tetramethylethylenediamine, after stirring and dispersing evenly, immediately pour the casting liquid B into a dry and clean glass plate, scrape a li...

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Abstract

The invention provides a preparation method of high-strength hydrogel serving as a cartilage substitute and capable of combining with the subchondral bone. The preparation method includes: dissolvingsodium silicate, acrylamide, a cross-linking agent and sodium alginate in water to obtain a mixed solution A, dissolving soluble phosphate, the sodium silicate, the acrylamide, the cross-linking agentand the sodium alginate in the water to obtain casting membrane liquid B, scraping the casting membrane liquid B on a glass plate to form a membrane by a membrane scraping rod, pouring the mixed solution A on the membrane, flattening by scraping with the membrane scraping rod, allowing nitrogen to flow in, utilizing ultraviolet to initiate acrylamide polymerization, subjecting the membrane, alongwith the glass plate, to crosslinking in aqueous calcium ion solution, soaking in aqueous gluconic acid-delta-lactone solution to obtain high-strength hybridized hydrogel, tearing the high-strength hybridized hydrogel membrane off the glass plate, and subjecting the high-strength hybridized hydrogel membrane to crosslinking in the aqueous calcium ion solution to obtain the high-strength hybridized hydrogel which comprises calcium phosphate in a lower layer. Due to the fact that the calcium phosphate has the biological activity of inducing in-vivo bone growth, the hydrogel is capable of combining with the subchondral bone.

Description

technical field [0001] The invention relates to a preparation method of a high-strength hydrogel cartilage substitute capable of combining with subchondral bone, belonging to the field of biological materials. Background technique [0002] Osteoarthritis caused by articular cartilage damage caused by trauma or disease has become the number one disabling disease in the world. According to the latest statistics on October 12, 2017, there are 355 million arthritis patients worldwide. Because there are no blood vessels in the cartilage to provide nutrition, the migration ability of chondrocytes is poor, and the articular cartilage cannot repair itself after damage. At present, clinical treatment of articular cartilage injuries is mainly based on surgery, including osteotomy, arthroscopic repair, autologous cartilage transplantation, and arthrodesis. These treatments can only relieve pain and restore joint function in the short term, but cannot replace the biological properties ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/16A61L27/12A61L27/02A61L27/50A61L27/52A61L27/54
CPCA61L27/025A61L27/12A61L27/16A61L27/20A61L27/50A61L27/52A61L27/54A61L2300/112A61L2400/12A61L2430/06C08L5/04C08L33/26
Inventor 赵孔银齐梦白甜王力鑫张天烨周鑫吴梦迪刘博华魏俊富
Owner TIANJIN POLYTECHNIC UNIV
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