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Cassava reside liquid feed and preparation method thereof

A technology of liquid feed and cassava residue, applied in animal feed, animal feed, application and other directions, can solve the problems of chaotic fermentation enzyme ratio, long fermentation cycle and high production cost, and achieve short fermentation cycle, low production cost and reduce pollution. Effect

Inactive Publication Date: 2018-11-06
HUBEI XIPU BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method uses many types of fermentation strains and enzymes for enzymolysis, and the combination of each strain and each fermentation enzyme is chaotic, and the production cost is high and the fermentation period is long.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0033] The preparation method of Candida albicans seed liquid: Weigh 13.1g of malt extract powder and add water to make up to 1000mL, sterilize at 115°C for 15 minutes to inoculate S.

[0034] Preparation method of Aspergillus niger seed solution: Weigh 200g of potatoes, peel them, cut into pieces, boil in distilled water for 30min, filter after cooling and take the filtrate to 1000mL, add 3g of potassium dihydrogen phosphate, 1.5g of magnesium sulfate , 20g of glucose, 10mg of vitamin B1, 20g of agar, sterilized at 121°C for 15 minutes to inoculate Aspergillus niger, and cultivated on a shaker at 120-160rpm and 28°C for 18-20h.

[0035] The preparation method of Lactobacillus plantarum seed liquid: weigh 10.0g of casein peptone, 10.0g of beef extract, 5.0g of yeast powder, 5.0g of glucose, 5.0g of sodium acetate, 2.0g of diammonium citrate, 1.0g Tween 80, 2.0g K 2 HPO 4, 0.2g MgSO 4 .7H 2 O, 0.05g MnSO 4 .H 2 O, 20.0 g CaCO 3 , add water to the volume to 1000mL, adjust...

Embodiment 1

[0037] The preparation method of the cassava residue liquid feed of the present embodiment may further comprise the steps:

[0038] 1) Take 100kg of wet cassava dregs, the mass fraction of moisture contained in the wet cassava dregs is 75%, put it into a stainless steel fermentation tank, stir while feeding water vapor, heat to 80°C and keep it warm for 0.5h at 80°C , keep stirring during the heat preservation period. After the incubation, the temperature was lowered to 60° C., 30000 U of α-amylase was added, and the first-level enzymatic hydrolysis was carried out by incubating for 0.5 h to obtain the first-level enzymatic hydrolyzate.

[0039] 2) Cool the primary enzymatic hydrolyzate in step 1) to 50°C and adjust the pH value to 4 with acetic acid, then add 200,000 U of pectinase and 1,800,000 U of cellulase and incubate for 1 hour for secondary enzymatic hydrolysis to obtain secondary Enzymolyzate.

[0040] 3) Cool the secondary enzymolyzate in step 2) to 25° C., add 0.1...

Embodiment 2

[0045] The preparation method of the cassava residue liquid feed of the present embodiment may further comprise the steps:

[0046] 1) Take 100kg of wet cassava residue, the mass fraction of moisture contained in the wet cassava residue is 75%, put it into an electric heating pot with a sandwich, stir while heating, heat to 80°C and keep it warm at 75°C for 1h , keep stirring during the heat preservation period. After the heat preservation, the temperature was lowered to 55° C., 100,000 U of α-amylase was added, and the heat preservation was carried out for 1 hour for primary enzymatic hydrolysis to obtain a primary enzymatic hydrolyzate.

[0047] 2) Cool the primary enzymatic hydrolyzate in step 1) to 45°C and adjust the pH value to 5 with acetic acid, then add 180,000 U of pectinase and 2,000,000 U of cellulase and incubate for 0.5 h for secondary enzymatic hydrolysis to obtain two grade enzyme hydrolyzate.

[0048] 3) Cool the secondary enzymatic hydrolyzate in step 2) to...

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PUM

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Abstract

The invention relates to a cassava reside liquid feed and a preparation method thereof, and belongs to the technical field of feeds. The preparation method of the cassava reside liquid feed comprisesthe following steps: taking wet cassava residues, heating the cassava residues to 70-80 DEG C, carrying out thermal insulation for 0.5-1 hour, cooling the cassava residues to 55-65 DEG, adding alpha-amylase, and carrying out thermal insulation for 0.5-1 hour so as to obtain a first-grade enzymatic hydrolysate; cooling the first-grade enzymatic hydrolysate to 45-55 DEG C, regulating the pH value to4-5, adding pectinase and cellulase, and carrying out thermal insulation for 0.5-3 hours so as to obtain a second-grade enzymatic hydrolysate; cooling the second-grade enzymatic hydrolysate to 25-35DEG C, adding calcium carbonate, aspergillus niger, candida utilis as well as one or both selected from urea and ammonium sulfate, and carrying out fermentation for 4-12 hours so as to obtain a first-grade fermentation product; heating the first-grade fermentation product to 35-40 DEG C, adding lactobacillus plantarum, and carrying out fermentation for 4-12 hours so as to obtain a second-grade fermentation product; and then, adding auxiliary materials into the second-grade fermentation product. The preparation method of the cassava reside liquid feed is relatively short in fermentation cycle and relatively low in production cost; and the prepared cassava reside liquid feed is balanced in nutrients. Moreover, cassava residues are fully utilized, so that pollution of the cassava residues onthe ecological environment is reduced.

Description

technical field [0001] The invention belongs to the technical field of feed, and in particular relates to a cassava residue liquid feed and a preparation method thereof. Background technique [0002] Cassava residue is a by-product of cassava starch extraction, and a large amount of fresh cassava residue that is not processed in time is easy to rot and deteriorate, causing great pollution to the environment. The wastewater contained in cassava residues will also cause great damage to vegetation and soil. How to use cassava residues has become an urgent problem to be solved. [0003] Cassava residue has the characteristics of high moisture, high fiber, and low protein, and its crude fiber has strong stability. When it is directly fed to animals, its digestibility is low and its nutrition is extremely unbalanced. Its moisture content is usually more than 75%. Drying to below 12% moisture content will cost more than 600 yuan per ton. The drying cost is very high. It is not an ...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/14
CPCA23K10/12A23K10/14
Inventor 程林春王俊青赵大伟恽辉余忠丽劳泰财李伟霞
Owner HUBEI XIPU BIOTECH
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