Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation of zearalenone non-toxic electrochemical luminescence sensor based on peptide sensor

A zearalenone, electrochemical technology, applied in chemiluminescence/bioluminescence, electrochemical variables of materials, analysis by chemical reaction of materials, etc., can solve problems such as limiting wide application, achieve high sensitivity, excellent Effects of Electron Transfer Function

Active Publication Date: 2018-11-13
福建省妇幼保健院
View PDF5 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Electrochemiluminescent immunosensors, a type of biosensor that utilizes the specific binding between antigens and antibodies, have the advantages of high sensitivity, good selectivity, easy operation, easy miniaturization, continuous and rapid detection and analysis, etc. In the detection process of ZEA based on electrochemiluminescence immunosensor, ZEA antigen standard solution will inevitably be used, which will cause serious harm to the operator's body, thus limiting the wide application of this method to a certain extent

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation of zearalenone non-toxic electrochemical luminescence sensor based on peptide sensor
  • Preparation of zearalenone non-toxic electrochemical luminescence sensor based on peptide sensor
  • Preparation of zearalenone non-toxic electrochemical luminescence sensor based on peptide sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. A method for preparing a nontoxic electrochemiluminescent competitive immunosensor based on a peptide sensor of zearalenone:

[0032] (1) The glassy carbon electrode (GCE) is first mechanically polished and polished on the suede covered with alumina powder, the residual powder on the surface is washed with secondary water, and then moved into an ultrasonic water bath for cleaning until it is cleaned, and finally ethanol is used in sequence, Wash thoroughly with dilute acid and water;

[0033] (2) Add 5 μL of polyvinylpyrrolidone ( PVP ) with a concentration of 4 mg / ml dropwise to assist the synthesis of TiO 2 mesogen with 100 mu L 0.1wt% chitosan (CS) mixed solution was placed on the surface of a clean glassy carbon electrode, dried under an infrared lamp, and cooled to room temperature to obtain ps-TiO 2 MC / CS modified glassy carbon electrode;

[0034] (3) 5 μL of 5.0 wt.% glutaraldehyde (GA) solution was added dropwise to activate the surface of the modified el...

Embodiment 2

[0040] The TiO synthesized assisted by polyvinylpyrrolidone (PVP) used in the above Example 1 2 mesogen ( ps-TiO 2MC) was prepared by the following method: 1 g of polyvinylpyrrolidone (PVP) was dissolved in 100 ml of 4.2 mol / L nitric acid (HNO3) solution, and then 4 g of sodium dodecyl sulfate (SDS) was added to the above solution , stirred for a few minutes under the action of ultrasound, then added 2ml of titanium isopropoxide (titanium(IV) isopropoxide (TIP)), stirred at 70 °C for 48 h, and the samples obtained by centrifugation were washed with distilled water and ethanol in turn , and then dried overnight at 60 °C; finally, in an argon atmosphere, they were calcined at 400 °C for 120 min and 600 °C for 180 min, respectively, to obtain ps-TiO 2 MC, see figure 1 A in is the transmission electron microscope image (TEM) of titanium dioxide (TiO2), figure 1 B in is the scanning electron microscope image (SEM) of titanium dioxide (TiO2), figure 1 C in is the N2 adsorptio...

Embodiment 3

[0042] The electrochemiluminescence probe (peptide / Nafion-Ru(bpy) used in the above-mentioned embodiment 1 3 2+ / cysteamin) was prepared by the following method: 1) 20 mmol / L cysteamine (cysteamine) solution with a volume ratio of 1:1 and nickel-iron nanotubes (NiFe 2 o 4 NT S ) solution was mixed and shaken at room temperature for 6 h, washed and centrifuged to obtain cysteamine@NiFe 2 o 4 NTs complex solution and redispersed it in deionized water; 2) Mix 0.05% Nafion with 1.0×10 -3 mol / L ruthenium bipyridine (Ru(bpy) 3 2+ ) was added to the complex solution prepared above, mixed and shaken for 5 h, centrifuged, washed and redispersed to obtain Nafion-Ru(bpy) 3 2+ / cysteamine@NiFe 2 o 4 NTs complex; 3) First add 200 μL 5.0 wt.% GA to Nafion-Ru(bpy) 3 2+ / cysteamine@NiFe 2 o 4 NTs mixed solution, mixed and shaken for 2 h, washed and centrifuged to remove excess GA reagent, then added 100 μL of 50 μg / mL peptide to the solution, shaken gently for 5 h, centrifu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses preparation of a zearalenone non-toxic electrochemical luminescence sensor based on a peptide sensor. The sensor takes TiO2 mesocrystal (ps-TiO2 MC) and chitosan (CS) synthesized through assistance of polyvinylpyrrolidone as a sensing platform, and takes a mercaptoethylamine functionalized ferronickel nano tube as a stand, and the large specific surface area of the ferronickel nano tube can provide more absorption sites for a luminescence agent ruthenium tris(bipyridine) (Ru(bpy)32+), and can fix a large amount of peptide with the effects of simulating zearalenone. Based in the advantages, the non-toxic competitive type electrogenerated chemiluminescence immunosensor is constructed, high-sensitivity detection of zearalenone is achieved, the sensor has the advantagesthat the sensitivity is high and the stability is good, and the sensor has the important application value and practical meaning at the aspect of clinical application.

Description

technical field [0001] The invention belongs to the technical field of novel functional materials and biosensing detection, and in particular relates to a preparation method and application of a non-toxic electrochemiluminescence competitive immunosensor based on a peptide sensor of zearalenone. Background technique [0002] Zearalenone (ZEA) is considered to be the most widely distributed miscellaneous mycotoxin, which is mainly the fungus of various Fusarium species, such as f. culmorum, f. (f.cericiisand), Fusarium equiseti (f.equiseti) and other strains. ZEA is a potential health hazard to both humans and animals. Even a small amount of food containing ZEA is eaten by animals and humans, and it can cause various serious and even fatal diseases, such as ovarian dysfunction, embryonic development and destruction. reproductive organs etc. Although there are a series of methods for the detection of ZEA, such as fluorescence polarization immunoassay (FPI), fluorescence, and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N27/30
CPCG01N21/76G01N27/308
Inventor 郑祥钦衣欢戴宏颜建英林丹玫宋建榕林元房丹丹
Owner 福建省妇幼保健院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com