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Enrichment method and application of unsaturated fatty acid of animal marrow grease

A technology of unsaturated fatty acids and mixed fatty acids, which is applied in the fields of fatty acid production, fat oil/fat production, fat oil/fat refining, etc. It can solve the problem of easy inactivation of effective ingredients in bone marrow, meet the requirements of advanced equipment and speed up the dissolution rate , good yield effect

Inactive Publication Date: 2018-11-16
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods do not separate the bone marrow from the bone, and the active ingredients of the bone marrow are easily inactivated during processing

Method used

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  • Enrichment method and application of unsaturated fatty acid of animal marrow grease
  • Enrichment method and application of unsaturated fatty acid of animal marrow grease
  • Enrichment method and application of unsaturated fatty acid of animal marrow grease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Bovine bone marrow powder is used as raw material and extracted with petroleum ether, where the bovine bone marrow powder can be replaced by sheep bone marrow powder, horse bone marrow powder or camel bone marrow powder;

[0038] a. Take animal bone samples and place them in a refrigerator at -4°C for 4 hours to harden the bone marrow into blocks. Separate the bone and bone marrow with a hand-held cutter and knife, collect the bone marrow, and wash it with distilled water for 3 times to remove blood stasis and impurities. After that, store it in a refrigerator at -20°C for 4 hours to harden the bone marrow into pieces, cut it into pieces, freeze and pulverize it with liquid nitrogen, and obtain bone marrow powder;

[0039] b. Wrap 100g of the bone marrow powder obtained in step a in a filter paper tube, put it into the extraction tube, connect the round bottom beaker, add 5 times the amount of petroleum ether on the extraction tube, connect the condenser tube, place it i...

Embodiment 2

[0045] Bovine bone marrow powder is used as raw material and extracted with n-hexane, where the bovine bone marrow powder can be replaced by sheep bone marrow powder, horse bone marrow powder or camel bone marrow powder;

[0046] a. Take animal bovine bone samples and place them in a -4°C refrigerator for 4 hours to harden the bone marrow into blocks. Separate the bone and bone marrow with a hand-held cutter and knife, collect the bone marrow, and wash it with distilled water for 5 times to remove blood stasis and bone marrow. After the debris, store in a refrigerator at -20°C for 8 hours to harden the bone marrow into pieces, cut it into pieces, freeze and pulverize it with liquid nitrogen, and obtain bovine bone marrow powder;

[0047] b. Wrap 100 g of the bovine bone marrow powder obtained in step a in a cloth tube, put it into the extraction tube, connect the round bottom beaker, add 5 times the amount of n-hexane on the extraction tube, connect the condensation tube, and p...

Embodiment 3

[0053] Bovine bone marrow powder is used as raw material and extracted with anhydrous ethanol, wherein the bovine bone marrow powder can be replaced by sheep bone marrow powder, horse bone marrow powder or camel bone marrow powder;

[0054] a. Take animal bone samples and place them in a -4°C refrigerator for 4 hours to harden the bone marrow into blocks. Separate the bone and bone marrow with a hand-held cutter and knife, collect the bone marrow, and wash it with distilled water for 4 times to remove blood stasis and impurities. After that, store it in a refrigerator at -20°C for 6 hours to harden the bone marrow into pieces, cut it into pieces, freeze and pulverize it with liquid nitrogen, and obtain the bone marrow powder;

[0055] b. Wrap 100 g of the bone marrow powder obtained in step a in a filter paper tube, put it into the extraction tube, connect the round bottom beaker, add 5 times the amount of absolute ethanol on the extraction tube, connect the condenser tube, and...

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Abstract

The invention provides an enrichment method and application of the unsaturated fatty acid of animal marrow grease. According to the method, animal marrow is washed with distilled water to remove bloodstasis and impurities, the marrow is stored in a refrigerator and hardened into a block, liquid nitrogen is utilized for grinding, n-hexane is adopted for extraction, pressure is decreased to recoversolvent, filtration is carried out, potassium hydroxide methanol solution is used for saponification and reflux for 2 hours, hydrochloric acid is added to acidify unsaponified matter, the grease layer is extracted and washed with water to become neutral, dehydration is carried out, rotary evaporation is carried out, so that mixed fatty acid is obtained, and urea inclusion or freezing crystallization is then respectively adopted to obtain the unsaturated fatty acid of animal marrow grease from the mixed fatty acid. By testing, the content of unsaturated fatty acid is increased to 69.22 percent, the proportion of saturated fatty acid / unsaturated fatty acid is 1 / 2.250, and antibacterial and anti-oxidation activity is remarkably improved. The unsaturated fatty acid of animal marrow grease prepared by the method is highly pure, clear and transparent and in the liquid state, has high bioactivity, and can be used as an important core material to prepare materials and microcapsules for drugs,health-care products or cosmetics.

Description

technical field [0001] The invention relates to a preparation method and application of animal bone marrow unsaturated fatty acid. Background technique [0002] Animal bones are composed of periosteum, bone and bone marrow. The main chemical components are protein, oil, minerals and so on. Among them, bone marrow contains a large amount of oil, generally the oil content reaches 90-95%, and the available resources are quite abundant. In the early stage, there were many studies on its protein components. The research on the chemical components and biological activities of oils and fats contained in the remaining oil after protein extraction or bone marrow itself has not been in-depth, and the mechanism of action and structure-activity relationship are unclear, resulting in low secondary utilization of resources. Byproduct - bone marrow resources remain underutilized. [0003] Xinjiang is one of the five traditional pastoral areas in my country and has extremely rich animal ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/10C11B3/12C11C3/00
CPCC11B1/10C11B3/12C11C3/00
Inventor 阿布力米提·伊力帕尔哈提·柔孜阿吉艾克拜尔·艾萨
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI