Bacterial strain CH18 capable of generating anthocyanidin and application thereof

A technology of CH18 and anthocyanin, applied in the field of anthocyanin-producing strain CH18, can solve problems such as no reports, and achieve the effects of wide suitable range, simple process and easy cultivation.

Active Publication Date: 2018-11-16
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports on the use of microorganisms to produce anthocyanins at home and abroad.

Method used

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  • Bacterial strain CH18 capable of generating anthocyanidin and application thereof
  • Bacterial strain CH18 capable of generating anthocyanidin and application thereof
  • Bacterial strain CH18 capable of generating anthocyanidin and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Screening and identification of bacterial strain CH18

[0036] 1. Isolation and purification of strain CH18

[0037] (1) Collect multiple soils from different regions, and then dilute with saline according to the gradient of 1:10, 1:100, 1:1000, 1:10000; 1:100000. Take 100-200ul dilutions and spread them on beef extract peptone medium, potato solid medium, Martin's solid medium, and inorganic salt solid medium, and cultivate them at 20°C-37°C, and set three for each dilution. parallel.

[0038] (2) Take the colonies grown on the above-mentioned plate, mark and separate them on potato culture medium, beef extract peptone medium, Martin's solid medium, and inorganic salt solid medium, cultivate them at room temperature for 2-8 days, and pick colored ones. For colonies, streak on potato medium, beef extract peptone medium, and Martin's solid medium until a single colony is obtained, and store on the slant of the test tube.

[0039] The medium formulation used...

Embodiment 2

[0074] Example 2: Fermentation culture of strain CH18 and determination of anthocyanins

[0075] 1. Cultivation and fermentation of strain CH18

[0076] 1. Cultivation of strain CH18

[0077] The study found that the strain CH18 has a wide range of suitable growth, can grow at a pH of 2-12, and a temperature of 20°C-37°C, and has a wide acid-base tolerance. At the same time, it can grow in a variety of media, such as Martin's medium, potato medium, and inorganic salt medium. Usable carbon sources include glucose, sucrose, maltose, starch, oat flour, corn flour, and the like. In addition, strain CH18 can also grow on at least one or several combined substrates of food and agricultural solid waste. The food raw materials referred to here include rice, corn, wheat, millet, oats, etc.; the agricultural solid waste includes straw, bran, sawdust, rice husk, etc.

[0078] 2. Liquid fermentation of strain CH18

[0079] (1) Strain activation

[0080] Pick the strain CH18 stored o...

Embodiment 3

[0100] Example 3: Bacteriostatic experiment of bacterial strain CH18 and its metabolites

[0101] 1. Toxicity test of strain CH18 and its fermentation products to bacteria

[0102] (1) Preparation of strain CH18 fermentation broth and solid fermentation product

[0103] Preparation of fermentation broth of strain CH18: Take 4mL-10ml of fermentation broth of strain CH18 and centrifuge at 800R for 10min to obtain supernatant, which should be used for antibacterial activity determination.

[0104] Preparation of the solid fermentation product of the strain CH18: absorb the bacterial liquid CH18 to inoculate grain or inoculate one or more mixed substrates among the agricultural solid waste substrates, culture at 20-37°C for 5-20 days, and take samples. Determination of antibacterial activity.

[0105] (2) configuration of antibiotic control group solution:

[0106] Weigh 20.0mg aureomycin, place in 100mL distilled water, and make 200mg / L aureomycin solution.

[0107] (3) Activ...

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PUM

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Abstract

The invention relates to a bacterial strain CH18 capable of generating anthocyanidin and an application thereof, which belong to the technical field of microbe. The bacterial strain CH18 through identification is Fusarium oxysporum, and a preservation number is CGMCC NO.14784. The bacterial strain has the advantages of wide adapting scope and easy culture, and can use agricultural solid waste, a grain raw material and a plurality of mediums to generate anthocyanidin through fermentation, and has the advantages of simple process, low cost, short production period, high anthocyanidin output, andwhole year production. The bacterial strain CH18 and its fermentation product have no biological toxicity, and is green and environmentally friendly. The anthocyanidin can be widely used for the fields of medical treatment, food, cosmetic, hair protection, and dental caries prevention, and has wide application prospect.

Description

technical field [0001] The invention relates to an anthocyanin-producing strain CH18, which belongs to the field of microbial fermentation and metabolites. Background technique [0002] Anthocyanidin belongs to flavonoid compounds and has a variety of biologically active functions, such as anti-oxidation, scavenging free radicals, anti-cancer, anti-radiation, preventing early generation of skin wrinkles, anti-inflammatory, lowering blood sugar, preventing cardiovascular diseases, treating intestinal It is safe and non-toxic, and is widely used in medicine, cosmetics, health products, food and other fields. [0003] Anthocyanin is a water-soluble pigment that exists in the surface cells and lower epidermis of plant petals, fruits, stems and leaves. It is one of the main pigments that constitute the color of petals and fruits. It can change color with the pH of the solution. , the acid turns red, and the alkali turns purple, making the petals colorful. [0004] At present, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02C12Q1/04C12P17/06C12R1/77
CPCC12N1/02C12N1/20C12P17/06C12Q1/04C12N1/145C12R2001/77G01N2333/37
Inventor 李艳菊侯慧鹏陶慧杨正礼张爱平
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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