Bacterial strain CH18 capable of generating anthocyanidin and application thereof
A technology of CH18 and anthocyanin, applied in the field of anthocyanin-producing strain CH18, can solve problems such as no reports, and achieve the effects of wide suitable range, simple process and easy cultivation.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0035] Example 1: Screening and identification of bacterial strain CH18
[0036] 1. Isolation and purification of strain CH18
[0037] (1) Collect multiple soils from different regions, and then dilute with saline according to the gradient of 1:10, 1:100, 1:1000, 1:10000; 1:100000. Take 100-200ul dilutions and spread them on beef extract peptone medium, potato solid medium, Martin's solid medium, and inorganic salt solid medium, and cultivate them at 20°C-37°C, and set three for each dilution. parallel.
[0038] (2) Take the colonies grown on the above-mentioned plate, mark and separate them on potato culture medium, beef extract peptone medium, Martin's solid medium, and inorganic salt solid medium, cultivate them at room temperature for 2-8 days, and pick colored ones. For colonies, streak on potato medium, beef extract peptone medium, and Martin's solid medium until a single colony is obtained, and store on the slant of the test tube.
[0039] The medium formulation used...
Embodiment 2
[0074] Example 2: Fermentation culture of strain CH18 and determination of anthocyanins
[0075] 1. Cultivation and fermentation of strain CH18
[0076] 1. Cultivation of strain CH18
[0077] The study found that the strain CH18 has a wide range of suitable growth, can grow at a pH of 2-12, and a temperature of 20°C-37°C, and has a wide acid-base tolerance. At the same time, it can grow in a variety of media, such as Martin's medium, potato medium, and inorganic salt medium. Usable carbon sources include glucose, sucrose, maltose, starch, oat flour, corn flour, and the like. In addition, strain CH18 can also grow on at least one or several combined substrates of food and agricultural solid waste. The food raw materials referred to here include rice, corn, wheat, millet, oats, etc.; the agricultural solid waste includes straw, bran, sawdust, rice husk, etc.
[0078] 2. Liquid fermentation of strain CH18
[0079] (1) Strain activation
[0080] Pick the strain CH18 stored o...
Embodiment 3
[0100] Example 3: Bacteriostatic experiment of bacterial strain CH18 and its metabolites
[0101] 1. Toxicity test of strain CH18 and its fermentation products to bacteria
[0102] (1) Preparation of strain CH18 fermentation broth and solid fermentation product
[0103] Preparation of fermentation broth of strain CH18: Take 4mL-10ml of fermentation broth of strain CH18 and centrifuge at 800R for 10min to obtain supernatant, which should be used for antibacterial activity determination.
[0104] Preparation of the solid fermentation product of the strain CH18: absorb the bacterial liquid CH18 to inoculate grain or inoculate one or more mixed substrates among the agricultural solid waste substrates, culture at 20-37°C for 5-20 days, and take samples. Determination of antibacterial activity.
[0105] (2) configuration of antibiotic control group solution:
[0106] Weigh 20.0mg aureomycin, place in 100mL distilled water, and make 200mg / L aureomycin solution.
[0107] (3) Activ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com