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A method for preparing chitin by fermentation

A technology of chitin and fermentation medium, applied in the field of bioengineering, can solve the problems of difficult industrialization, high cost, and low yield, and achieve the effects of stable product quality, low production cost, and high yield

Active Publication Date: 2021-07-02
SHANGHAI HAOHAI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the above-mentioned technical problems in the prior art, the present invention provides a method for preparing chitin by fermentation, and the method for preparing chitin by fermentation should solve the problems in the prior art. The method for preparing chitin has low yield and high cost, and is difficult to realize the technical problems of industrialization

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  • A method for preparing chitin by fermentation
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Examples

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Embodiment 1

[0028] The preserved strain of the present invention is isolated from farmland soil in Fengxian District, Shanghai, and separated by 6 generations of streaking to obtain the pure strain MF010. The biological characteristics of the strain are: the diameter of the colony is about 4.5cm; the mycelium is white, and a large number of light yellow to yellow spores grow on the surface of the colony after maturity; the colony is velvety, with radial grooves; no exudate; red. Conidia and conidiophores are yellow; conidia are nearly spherical and slightly rough; the surface of the colony is fully fertile; the optimum growth temperature is 28°C; aerobic; after molecular biological analysis of the 18sDNA conserved sequence of the strain, it was compared with GenBank Compared with the 18S rDNA sequence homology, it was found that the highest similarity with Aspergillus strains reached 99%, and it was identified as Aspergillus ochraceus. On April 25, 2018, the deposit number is CGMCC No.15...

Embodiment 2

[0030] (1) Incline cultivation

[0031]Draw a certain amount of bacterial solution evenly on the slant culture medium under aseptic operation to absorb the bacterial species preserved in the glycerol tube, and culture at a constant temperature of 28°C for 5-7 days. In the slant medium used, each liter of medium contains 200g of peeled potatoes, 20g of glucose, and 20g of agar, with a pH of 6.4-6.6; the above-mentioned medium is sterilized at 121°C for 30 minutes, and used after cooling.

[0032] (2) Seed cultivation

[0033] The seed medium used contained 8g of sucrose, 8g of yeast extract, and 2.0g of molasses per liter of medium, with a pH of 6.0; the above-mentioned medium was sterilized at 121°C for 30 minutes, and used after cooling.

[0034] Scrape the yellow spores from the slant bacteria in step (1) under aseptic operation and transfer them to sterile saline, and adjust the spore concentration to 4.0-7.0×10 8 spores / ml, then transferred to sterilized seed medium acco...

Embodiment 3

[0042] (1) Incline culture and seed culture

[0043] As in previous example 1

[0044] (2) Fermentation culture

[0045] The fermentation medium used contains 30g of sucrose, 20g of yeast extract, 8g of soybean powder, 1.0g of molasses, and 1.5g of K in every liter of medium. 2 HPO 4 , 1.4g MgSO 4 ·7H 2 O, 0.01 g FeSO 4 ·7H 2 O, 1.5 g NaH 2 PO 4 12H 2 O, pH 5.5; the above-mentioned medium was sterilized at 121°C for 30 minutes by moist heat, and then used after cooling.

[0046] Prepare 10 bottles of fermentation shake flasks with a volume of 250ml (each fermentation bottle contains 90ml sterile medium), in each fermentation shake flask, add a mass percent concentration of 20% hydrogen peroxide solution, add 0.8% per liter of culture medium The hydrogen peroxide solution of 1 ml, shake well, absorb 10 ml of the seed liquid cultivated in the step (2) and inoculate it in the fermentation shaker flask (10% inoculum size) aseptically, place it in a constant temperature s...

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Abstract

The invention provides a method for preparing chitin by fermentation, using Ochraus ochrae of CGMCC No.15668 as the strain, including a step of strain activation, a step of seed cultivation, a step of fermentation and cultivation, and a step of The step of obtaining the bacterium is a step of separating and extracting chitin. The present invention utilizes Aspergillus ochrae to ferment and produce chitin, and the fermentation raw materials used do not have animal-derived raw materials, so the final chitin product obtained has no risk of pyrogen pollution. The invention uses an oxidant to intensify the fermentation process, so that the final chitin yield is increased from 2.79g / L to 6.3g / L. Compared with the extraction process of shrimp and crab shells, the process of extracting chitin in the present invention only needs to use a lower concentration of acid and alkali to obtain chitin products with higher purity, which effectively reduces production costs and environmental pollution. Pressure, has certain industrial application prospects.

Description

technical field [0001] The invention belongs to the field of bioengineering and relates to a preparation method of chitin, in particular to a preparation method of chitin by fermentation. Background technique [0002] Chitin, also known as chitin, is a non-toxic, odorless white or off-white translucent solid, which is difficult to dissolve in water, dilute acid, dilute alkali and general organic solvents, thus limiting its application and development. . Later, people discovered in research and exploration that chitin is treated with concentrated alkali to remove its acetyl group and becomes soluble chitin, also known as chitosan or chitosan. Its chemical name is (1-4)-2 -Amino-2-deoxy-beta-D-glucose, or polyglucosamine for short. Due to the presence of a large number of amino groups in its macromolecular structure, this chitosan greatly improves the solubility and chemical activity of chitin, so it has a wide range of application values ​​in the fields of medical treatment...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/26C12N1/14C12R1/66
CPCC12P19/26C12N1/145C12R2001/66
Inventor 荣绍丰李理管世敏李茜茜张硕蔡宝国
Owner SHANGHAI HAOHAI BIOLOGICAL TECH
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