Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Detection method of content of tyramine in fermented vinegar

A detection method and technology for fermenting vinegar, which can be applied to measuring devices, instruments, scientific instruments, etc., can solve problems such as difficult operation, complicated pretreatment steps, etc., and achieve the effects of low detection limit, simple and convenient sample analysis, and simple processing process.

Active Publication Date: 2018-11-23
太原市宁化府益源庆力诚醋业有限公司
View PDF7 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The detection methods of biogenic amines include gas chromatography-tandem mass spectrometry, ion chromatography, high-performance liquid chromatography, thin-layer chromatography, and high-performance liquid chromatography-tandem mass spectrometry. The pretreatment steps of these methods are cumbersome and difficult to operate.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection method of content of tyramine in fermented vinegar
  • Detection method of content of tyramine in fermented vinegar
  • Detection method of content of tyramine in fermented vinegar

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (a) Pretreatment of sample solution: Take 10.0 mL of fermented vinegar and dilute it with deionized water 5 times, filter with 0.22μm microporous membrane; store the filtrate at 4°C for later use.

[0022] (b) Derivatization reaction of the sample: Take 100μL of the sample from the filtrate and add 2.0μg·mL -1 100μL of internal standard solution, followed by 100μL of saturated sodium bicarbonate adjusted to pH 10 with sodium hydroxide, 5.0mg·mL prepared with acetone -1 200μL of dansyl chloride was derivatized at 55°C and protected from light for 15min. After the end, 100 μL of ammonia was added to stop the reaction for 25 minutes. Add acetonitrile to make up to 1.0mL. 0.22μm microporous membrane filter. The filtrate is tested on the column.

[0023] (c) UPLC detection conditions: Waters Acquity Ⅰclass ultra performance liquid chromatograph, C18, 2.1×50mm, 1.7μm chromatographic column, mobile phases of deionized water and acetonitrile, flow rate 0.3mL·min -1 , UV detection ...

Embodiment 2

[0028] (a) Pretreatment of sample solution: Take 5.0 mL of fermented vinegar and dilute 20 times with deionized water, filter with 0.22μm microporous membrane; store the filtrate at 4°C for use.

[0029] (b) Derivatization reaction of the sample: Take 100μL of the sample from the filtrate and add 2.0μg·mL -1 100μL of internal standard solution, followed by 100μL of saturated sodium bicarbonate adjusted to pH 10 with sodium hydroxide, 3.0mg·mL prepared with acetone -1 200μL of dansyl chloride, derivatization reaction was performed at 50°C in the dark, and reacted for 20min. After the end, add 100 μL of ammonia to stop the reaction for 30 minutes. Add acetonitrile to make up to 1.0mL. 0.22μm microporous membrane filter. The filtrate is tested on the column.

[0030] (c) UPLC detection conditions: Waters Acquity Ⅰclass ultra performance liquid chromatograph, C18, 2.1×50mm, 1.7μm chromatographic column, mobile phases of deionized water and acetonitrile, flow rate 0.3mL·min -1 , UV de...

Embodiment 3

[0035] (a) Pretreatment of sample solution: Take 2.0 mL of fermented vinegar and dilute 50 times with deionized water, filter with 0.22μm microporous membrane; store the filtrate at 4°C for later use.

[0036] (b) Derivatization reaction of the sample: Take 100μL of the sample from the filtrate and add 2.0μg·mL -1 100μL of internal standard solution, then add 100μL of saturated sodium bicarbonate adjusted to pH 10 with sodium hydroxide, 2.0mg·mL prepared with acetone -1 200μL of dansyl chloride was derivatized at 60°C and protected from light for 10min. After the end, 100μL of ammonia was added to stop the reaction for 20 minutes. Add acetonitrile to make up to 1.0mL. 0.22μm microporous membrane filter. The filtrate is tested on the column.

[0037] (c) UPLC detection conditions: Waters Acquity Ⅰclass ultra performance liquid chromatograph, C18, 2.1×50mm, 1.7μm chromatographic column, mobile phases of deionized water and acetonitrile, flow rate 0.3mL·min -1 , UV detection wavelen...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a detection method of a content of tyramine in fermented vinegar, which belongs to the technical field of food analysis. The method is a precolumn derivatization-ultra performance liquid chromatography, and a Waters AcquityIclass ultra performance liquid chromatographic instrument C18 column is used. Compared with the literature, a method suitable for determining the tyramine in the fermented vinegar is established. The established method is simple and reliable and can be used for detecting the tyramine in the fermented vinegar; and the method is less in consumption of samples, short in time, good in method linearity, simple in operation, low in detection limit, high in sensitivity, and good in repeatability and recovery rate.

Description

Technical field: [0001] The invention relates to a food detection method, in particular to an ultra-high performance liquid chromatography method for detecting the content of tyramine in fermented vinegar. Background technique: [0002] Biological amines are mainly composed of aliphatic (putrescine, cadaverine, spermidine, spermine), aromatic (tyramine, β-phenethylamine) and heterocyclic groups (tryptamine, histamine), as a kind of physiological Active substances participate in metabolic activities in the organism. Biogenic amines are widely present in various foods, and relatively more in foods rich in protein and amino acids, especially fish and its products, cheese, meat products and fermented foods. Tyramine, also known as p-hydroxyphenethylamine, is a type of nitrogen-containing low-molecular-weight biological amine. Tyramine in food is mainly produced by decarboxylase produced by related microorganisms to catalyze the removal of the carboxyl group of tyrosine, or Aldehyde...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 张会香杨世军
Owner 太原市宁化府益源庆力诚醋业有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com