Microbial strain for treatment of heavy metal contaminated soil, screening method and application thereof

A technology of polluted soil and screening method, which is applied in the field of microorganisms, can solve problems such as pollution, unsatisfactory repair effect, and threats to the production and life of nearby residents, and achieve the effect of small soil disturbance, low cost, and strong tolerance

Active Publication Date: 2018-12-07
SUZHOU UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2011, a chromium slag pollution incident occurred in Qujing, Yunnan, China. The investigation found that the groundwater in the "Longtan" area on the southeast side of the Yunnan Luliang Chemical Industry Co., Ltd. plant may have been severely polluted, and the chromium at the groundwater outlet in this area exceeded the standard by 242 times. The chromium in the water in the field exceeds the standard by 126 times, and the water in the nearby river section is used for agricultural irrigation, which seriously threatens the production and life of nearby residents
At present, most of the restoration technologies that have received attention are in the stage of laboratory experiments, and the actual restoration effect is not ideal.

Method used

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  • Microbial strain for treatment of heavy metal contaminated soil, screening method and application thereof
  • Microbial strain for treatment of heavy metal contaminated soil, screening method and application thereof
  • Microbial strain for treatment of heavy metal contaminated soil, screening method and application thereof

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Effect test

Embodiment 1

[0030] The screening of microbial strains for processing heavy metal-contaminated soil of the present invention adopts a liquid phase enrichment method, comprising the following steps:

[0031] (1) Weigh 5g of contaminated soil samples (the soil is taken from Shangfangshan, Suzhou City, and finally contains Cd 2+ 50mg / kg, Cr 6+ 50mg / kg), added to the liquid medium containing beef extract peptone, then vibrated at 30°C and 160rpm to obtain a soil mixture;

[0032] (2) The soil mixture is transferred to a centrifuge tube, and the supernatant after centrifugation is taken as the source of cadmium passivation microorganisms;

[0033] (3) the supernatant that step (2) obtains is inoculated into the beef extract peptone liquid medium (Cd) that contains cadmium and chromium 2+ The content is 50mg / L, Cr 6+ content of 50mg / L), cultured with shaking at 30°C and 160rpm, centrifuged after the completion of the culture, sucked 2mL of the supernatant with a sterile pipette, and transferr...

Embodiment 2

[0043] The microorganism bacterial strain (2wt% fresh bacterium liquid) of screening of the present invention is inoculated in the beef extract peptone liquid culture medium through 121 ℃ of sterilization 30min, and wherein, beef extract peptone liquid culture medium comprises the component of following mass percentage: 1% Peptone, 0.5% beef extract, 0.5% sodium chloride, and the rest are deionized water, cultured (160rpm, 10h at 30°C) to the logarithmic growth phase to obtain the bacterial liquid; or freeze the bacterial liquid with a vacuum freeze dryer Dried and set aside.

Embodiment 3

[0045] Add different concentrations of Cd to beef extract peptone liquid medium (1% peptone, 0.5% beef extract, 0.5% sodium chloride, and the rest is deionized water) 2+ and Cr 6+ , sterilized at 121°C for 30min; then inoculated with the microbial strains screened by the present invention, cultivated at 30°C and 160rpm, and took samples at different times to measure the OD value; 2+ Concentrations were 5, 20, 50, 100mg / L, Cr 6+ When the concentrations were 5, 10, 20, and 50 mg / L, the strain entered the logarithmic growth phase at 0.5, 2, 4, and 18 hours respectively, and the highest OD values ​​that could be achieved were 5.89, 5.55, 5.42, and 3.50, respectively. From the data showed that the strain on Cd 2+ Still very well tolerated.

[0046] The above-mentioned inoculation of the microbial strains screened by the present invention is to get 2% fresh bacterial liquid, or to pick a single colony and insert it into the medium on the beef extract peptone solid medium. bacter...

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Abstract

The invention discloses a microbial strain for treatment of heavy metal contaminated soil, a screening method and application thereof. The screened microbial strain is Bacillus subtilis, and the preservation number is CCTCC NO:M 2017832. The microbial strain is inoculated into a medium for culture to a logarithmic growth period so as to obtain a bacterial liquid; and adding the bacterial liquid into the heavy metal contaminated soil, and the substances are mixed evenly to complete treatment of the heavy metal contaminated soil; or subjecting the bacterial liquid to vacuum freeze drying, then adding the treated bacterial liquid into soil, and mixing the substances evenly to complete treatment of the heavy metal contaminated soil; and immobilizing cadmium and chromium in the contaminated soil by passivation to reduce the bio-availability of cadmium and chromium and the absorption and utilization of cadmium and chromium by plants, thus reaching the purpose of remediating contaminated soil.

Description

technical field [0001] The invention relates to the field of microorganisms, and relates to a microbial strain for repairing heavy metal polluted soil, a screening method and application thereof. Background technique [0002] Heavy metals refer to metals with a specific gravity greater than 5. There are currently about 45 types, such as copper, lead, zinc, iron, cobalt, nickel, manganese, cadmium, mercury, tungsten, molybdenum, gold, silver, etc. A small amount of heavy metals such as copper, manganese, and zinc are elements necessary for life activities. Most heavy metals such as cadmium, mercury, lead, and chromium are not necessary for organisms, and excessive heavy metal elements are toxic to the human body. Heavy metals are widely distributed in the atmosphere, lithosphere and hydrosphere. Although it generally does not cause harm to humans in the natural environment, with the development of industry and cities, the problem of soil heavy metal pollution is becoming mor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/07
CPCB09C1/10C12N1/20C12N1/205C12R2001/07
Inventor 施维林史广宇葛嵩韩旭朱晓东侯静雯
Owner SUZHOU UNIV OF SCI & TECH
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