Anticoagulant hydrogel coating capable of catalyzing release of nitric oxide and preparation method of anticoagulant hydrogel coating capable of catalyzing release of nitric oxide

A technology of nitric oxide and blood water, applied in the field of medical materials, can solve the problems of inability to meet the anti-coagulation function, uncontrollable release of nitric oxide, etc., and achieve the effects of excellent stability, good anti-oxidation and dense structure

Active Publication Date: 2018-12-11
JILIN VENUS HAOYUE MEDICAL LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In view of the above-mentioned deficiencies in the prior art, the present invention provides an anticoagulant hydrogel coating capable of catalyzing the release of nitric oxide and a preparation met

Method used

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  • Anticoagulant hydrogel coating capable of catalyzing release of nitric oxide and preparation method of anticoagulant hydrogel coating capable of catalyzing release of nitric oxide
  • Anticoagulant hydrogel coating capable of catalyzing release of nitric oxide and preparation method of anticoagulant hydrogel coating capable of catalyzing release of nitric oxide

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Embodiment 1

[0029] A method for preparing an anticoagulant hydrogel coating that can catalyze the release of nitric oxide, comprising the following steps:

[0030] (1) Ultrasonic cleaning and drying of the stainless steel base material;

[0031] (2) Place the cleaned stainless steel in 0.1mM chitosan solution with a pH value of 6, react at room temperature for 2min, then take it out and wash it with deionized water for 3 times;

[0032] (3) The product obtained in step (2) was placed in a mixed solution with a pH value of 11, containing 1 mM tannic acid and 0.01 mM ebselenium, reacted at room temperature for 5 min, then took it out and washed it with deionized water for 3 times;

[0033] (4) Put the product obtained in step (3) in a 0.1 mM hyaluronic acid solution with a pH value of 11, react at room temperature for 1 min, then take it out and wash it with deionized water for 3 times;

[0034] (5) At room temperature, using the product obtained in step (4) as a substrate, repeat the oper...

Embodiment 2

[0036] A method for preparing an anticoagulant hydrogel coating that can catalyze the release of nitric oxide, comprising the following steps:

[0037] (1) cleaning and drying the titanium alloy base material;

[0038] (2) Place the cleaned titanium alloy in 0.01mM poly-L-arginine hydrochloride solution with a pH value of 7.4, react at room temperature for 2min, then take it out and wash it with deionized water for 3 times;

[0039] (3) Place the product obtained in step (2) at a pH of 7.2 in a mixed solution containing 0.1mM epigallocatechin gallate and 0.01mM cystamine, react at room temperature for 5min, then take it out and rinse with deionized water Wash 3 times;

[0040] (4) Put the product obtained in step (3) in a 0.1 mM polyglutamic acid solution with a pH value of 6.8, react at room temperature for 3 minutes, then take it out and wash it 3 times with deionized water;

[0041] (5) At room temperature, using the product obtained in step (4) as a substrate, repeat the...

Embodiment 3

[0043] A method for preparing an anticoagulant hydrogel coating that can catalyze the release of nitric oxide, comprising the following steps:

[0044] (1) cleaning and drying the polytetrafluoroethylene material;

[0045] (2) Place the cleaned polytetrafluoroethylene in 2mM poly-L-lysine hydrobromide solution with a pH value of 7.4, react at room temperature for 2min, then take it out and wash it with deionized water for 3 times;

[0046] (3) Place the product obtained in step (2) at a pH of 6.5 in a mixed solution containing 0.5mM epigallocatechin and 0.001mM selenocystine, react at room temperature for 2min, then take it out and rinse with deionized water Wash 3 times;

[0047] (4) Put the product obtained in step (3) in a 0.05 mM dextran solution with a pH value of 6.9, react at room temperature for 3 minutes, then take it out and wash it with deionized water for 3 times;

[0048] (5) At room temperature, using the product obtained in step (4) as a substrate, repeat the op...

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Abstract

The invention discloses an anticoagulant hydrogel coating capable of catalyzing the release of nitric oxide and a preparation method of the anticoagulant hydrogel coating capable of catalyzing releaseof nitric oxide. The method comprises the following steps: (1) pretreating a substrate material; (2) sequentially placing the pretreated substrate material in a polycation electrolyte, a mixed solution of polyphenol and a compound A, and a polyanion electrolyte at room temperature to react for 20 minutes, and washing for 3 to 5 times; (3) using a product obtained in step (2) as a substrate at room temperature, repeating the operation of step (2) for 5 to 20 times, after drying by nitrogen, obtaining the anticoagulant hydrogel coating capable of catalyzing the release of nitric oxide. The hydrogel coating prepared by the method disclosed by the invention has the advantages of simple method, stable structure, and good biocompatibility, can stably catalyze NO to release for a long time to protect endothelial cells and has an anticoagulant effect. The coating can be used for surface modification of cardiovascular stent materials and heart valves.

Description

technical field [0001] The invention belongs to the technical field of medical materials, and in particular relates to an anticoagulant hydrogel coating capable of catalyzing the release of nitric oxide and a preparation method thereof. The coating can be used for surface modification of cardiovascular internal stent materials and heart valves. Background technique [0002] With the increasing pressure of people's life and irregular work, rest and diet, cardiovascular disease in my country has far surpassed the threat to life and health of tumors and other diseases, and has become the number one killer of diseases and deaths. Cardiovascular materials mainly include vascular stents, artificial blood vessels, cardiovascular biological valves, hemodialyzers, etc. For a long time, cardiovascular materials have faced a major problem of anticoagulation, and excellent surface anticoagulant performance is the key to blood contact materials. Nitric oxide plays an important role in r...

Claims

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Application Information

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IPC IPC(8): A61L31/08A61L31/10A61L31/14A61L31/16A61L27/28A61L27/34A61L27/52A61L27/54A61L27/58
CPCA61L27/28A61L27/34A61L27/52A61L27/54A61L27/58A61L31/08A61L31/10A61L31/145A61L31/148A61L31/16A61L2300/216A61L2300/402A61L2300/41A61L2300/42A61L2300/602A61L2300/606A61L2400/18A61L2420/02A61L2420/06A61L2420/08A61L2430/20C08L5/08C08L77/04C08L5/02
Inventor 罗日方王云兵杨立张博
Owner JILIN VENUS HAOYUE MEDICAL LTD
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