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Polypeptide-silver nanometer cluster compound, and preparation method and applications thereof

A technology of nano-clusters and composites, applied in the biological field, can solve toxicity and other problems, and achieve the effects of uniform size, good antibacterial activity, and good biological safety

Active Publication Date: 2018-12-14
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, while nano-silver exhibits high-efficiency antibacterial activity, there are also some problems.
First of all, nano-silver produces antibacterial activity through the release of silver ions. While killing bacteria, it also produces toxicity to normal cells in surrounding tissues, and there are outstanding biological safety issues.

Method used

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  • Polypeptide-silver nanometer cluster compound, and preparation method and applications thereof
  • Polypeptide-silver nanometer cluster compound, and preparation method and applications thereof
  • Polypeptide-silver nanometer cluster compound, and preparation method and applications thereof

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Experimental program
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Effect test

Embodiment 1

[0046] Combine silver nitrate and peptide P + (Sequence is CCY-RKRKRK) Dissolve in distilled water at a molar ratio of 1:1, stir in a water bath at 37°C for 10 minutes, add 0.5 mol / L sodium hydroxide solution to make the solution pH 12; continue stirring 12 Light yellow transparent P + @AgNCs solution is dialyzed at room temperature for 12 hours to further remove excess silver ions, peptide molecules, sodium hydroxide and sodium borohydride that are not involved in the reaction, and obtain pure peptide-silver nanocluster complex P + @AgNCs solution, store at 4℃ for later use. Electron microscopy of the prepared compound, the results are as follows figure 2 The results show that the prepared P+@AgNCs has uniform size and good dispersion.

Embodiment 2

[0048] Dissolve silver nitrate and polypeptide KLA (sequence CCY-KLAKLAKKLAKLAK) in distilled water at a molar ratio of 1:1, stir in a water bath at 37°C for 10 minutes, add sodium borohydride to a concentration of 0.5μM, and then adjust the concentration to 0.5mol / L sodium hydroxide solution to make the pH of the solution 12; continue to stir for 12 hours to form a light yellow transparent KLA@AgNCs solution, dialyzed at room temperature for 12 hours to further remove the excess silver ions, peptide molecules, and hydroxide that did not participate in the reaction Sodium and sodium borohydride, etc., to obtain the pure polypeptide-silver nanocluster complex KLA@AgNCs solution, and store it at 4℃ for later use. Electron microscopy of the prepared compound, the results are as follows image 3 The results show that the prepared KLA@AgNCs has uniform size and good dispersion.

[0049] Functional Verification

[0050] 1. The antibacterial activity of the polypeptide-silver nanocluste...

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Abstract

The invention belongs to the technical field of biology, and especially relates to a polypeptide-silver nanometer cluster compound, and a preparation method and applications thereof. According to thepreparation method, the nanometer cluster compound is prepared from silver ions and a polypeptide, possesses excellent antibacterial activity and biological safety. It is shown by experiments that theminimum inhibition concentration of the polypeptide-silver nanometer cluster compound on Escherichia coli is 1<mu>g / mL, and the minimum inhibition concentration on staphylococcus aureus is 10<mu>g / L.

Description

Technical field [0001] The present invention relates to the field of biotechnology, in particular to a polypeptide-silver nanocluster complex and a preparation method and application thereof. Background technique [0002] Bacterial infection is an acute systemic infection caused by pathogenic bacteria or conditional pathogenic bacteria that invade the blood circulation to grow and multiply and produce toxins and other metabolites. In order to prevent bacterial infections, the research and development of antibacterial agents has increasingly become an important research direction. Since the discovery of antibiotics in the early twentieth century, the widespread use of antibiotics for nearly a century has spawned a series of drug-resistant super bacteria. In addition, the lack of broad-spectrum antibacterial effects of antibiotics has led to the abuse of antibiotics, especially in developing countries such as China. [0003] As a new generation of antibacterial agents, nanomaterial...

Claims

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Application Information

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IPC IPC(8): A61K33/38A61K47/42A61P31/04A23L33/18A23L33/16A01N59/16A01N37/46A01P1/00
CPCA01N37/46A01N59/16A23V2002/00A61K33/38A61K47/42A23L33/16A23L33/18A61P31/04A23V2250/1562A23V2250/55Y02A50/30
Inventor 马晓川费兴书葛翠翠
Owner SUZHOU UNIV
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