Method for enzymatically extracting rhodomyrtus tomentosa anthocyanidin by means of ultrasonic waves assisting in biological compound enzyme

Abstract

The invention discloses a method for enzymatically extracting rhodomyrtus tomentosa anthocyanidin by means of ultrasonic waves assisting in a biological compound enzyme. According to the method, firstly, rhodomyrtus tomentosa fruits are mashed into pulp, then the biological organized enzyme is added for enzymolysis, then through ultrasonic wave assistant extraction and centrifugation, supernate iscollected, ultrafiltration and concentration are conducted, and finally, a concentrated solution is subjected to spray drying to obtain the rhodomyrtus tomentosa anthocyanidin; the biological organized enzyme is a compound of acid cellulase, glucoamylase and tannase; meanwhile, blueberry anthocyanidin extracted by means of biological organized enzyme enzymolysis and ultrasonic wave assistant extraction is high in purity, low in cost and simple and convenient to operate; by means of citric acid, the stability of the anthocyanidin is improved, and anthocyanidin loss caused by oxidation of polyphenol and enzymes is effectively controlled; finally, the efficiency of extracting the anthocyanidin reaches 90% or above.

Description

technical field [0001] The invention relates to the technical field of extraction of plant functional natural active ingredients, in particular to a method for extracting myrtle anthocyanins by ultrasonic-assisted biocomposite enzyme enzymatic hydrolysis. Background technique [0002] Anthocyanins are a class of water-soluble pigments widely present in plants. They are polymers formed by condensation of a certain amount of catechin and epicatechin. They belong to flavonoid compounds and are natural water-soluble pigments. Anthocyanins widely exist in plant petals, cell fluid tissues of fruits and surface cells of stems and leaves. The anthocyanins contained in plants not only give them colorful colors, recent studies have shown that anthocyanins also have a variety of physiological functions, such as inhibiting platelet coagulation, preventing thrombosis, heart disease, anti-cancer, anti-inflammation, and delaying aging And so on. As a natural pigment, anthocyanins are non...

AI Technical Summary

Problems solved by technology

The principle of organic solvent extraction method is simple and requires less equipment, but most organic solvents have high toxicity and side effects and low extraction rate

Due to the longer extraction time of organic solvents, the thermally unstable components are easily destroyed, the impurity content is high, the environment is polluted, and the simple reagent extraction is difficult to fully extract the anthocyanins in plants. In recent years, it has been found that some Auxiliary methods, including microwave, ultrasonic, pressurized, supercritical and other physical techniques, to increase the yield of anthocyanins, shorten the extraction time, and improve the quality of the extract

[0005] Due to the currently known extraction methods of myrtle anthocyanins in China, only organic solvents are used for extraction, or some auxiliary methods are used to promote them. These methods generally have disadvantages such as low extraction rate, difficult extraction operation, high extraction cost, and high impurity content.