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Nanometer antibacterial agent for preventing and controlling plant fungal diseases

A technology for plant fungal diseases and nano-antibacterial agents, which can be used in plant growth regulators, fungicides, biocides, etc., and can solve problems such as high toxicity of non-target organisms

Inactive Publication Date: 2018-12-21
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a large number of drug-resistant strains have emerged in the prevention and control of these organic agents. In addition, organic agents are highly toxic to non-target organisms, and some non-degradable pesticides or organic metabolites accumulate in the environment or in animals and plants.

Method used

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  • Nanometer antibacterial agent for preventing and controlling plant fungal diseases
  • Nanometer antibacterial agent for preventing and controlling plant fungal diseases
  • Nanometer antibacterial agent for preventing and controlling plant fungal diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Prepare the nano antibacterial agent for preventing and treating plant fungal diseases, comprising the following steps:

[0031] (1) Weigh 500g of copper nitrate and 1L of industrial ammonia water (28% concentration) into the autoclave, then add 1L of deionized water to dilute. Heat to 220°C under airtight condition, and react for 3 hours.

[0032] (2) Filter the reacted mixed solution to obtain copper hydroxide precipitate and reaction solution. After washing and drying the copper hydroxide precipitate, place it in a microwave sintering furnace and rapidly raise the temperature to 600°C at 40°C / min temperature, heat-preserved and calcined for 30 minutes, cooled and ground to obtain nano-copper oxide powder.

[0033] (3) disposing the obtained nano-copper oxide powder into the reaction liquid in step (1), and fully stirring to form a certain concentration of nano-copper oxide suspension.

[0034] (4) Add 3% volume of TEA sodium lauryl sulfate and 5% volume of Atractyl...

Embodiment 2

[0036] This example is used to illustrate the inhibitory effect of the antibacterial agent involved in the present invention on the infection of plant leaves by fungal pathogens.

[0037] The rose hybrida variety 'Samantha' (Samantha) used in this experiment was obtained from the Flower Breeding Base of Nankou Farm, Changping District, Beijing. Cut off twigs with 4-5 sections each with 5 pinnate compound leaves, put them in clear water immediately, and bring them back to the laboratory for processing within 2 hours. Trim the base of the shoots in water, keep 3-4 pinnate compound leaves and put them in distilled water to balance them for later use. The strains used in this experiment are powdery mildew of rose, Podosphaera pannosa, P. pannosa, provided by the College of Horticulture, China Agricultural University.

[0038] Cut the dense rose leaves of powdery mildew into a 50ml centrifuge tube, add 20mL of sterile water, vortex fully oscillate, remove the leaves, transfer to a...

Embodiment 3

[0046] The experimental process of this embodiment is basically the same as that of Example 2, the difference is that after the infection process ends, the fungus is collected to infect the leaves, and the number and growth status of the mycelia on the leaves are observed with a scanning electron microscope (SEM, Phenom ProX, Phenom, NLD) .

[0047] The results show that the novel antifungal agent involved in the present invention inhibits the germination and growth of powdery mildew bacteria, and both concentrations of antibacterial agents inhibit the quantity and activity of powdery mildew bacteria. Transmission electron microscopy showed that the mycelium of powdery mildew was dense, strong and vigorous in the blank treatment; after the two concentrations of antifungal agents were treated, only a small amount of powdery mildew mycelium existed on the leaf surface, and the mycelium was slender and inactive ( image 3 ). Microscopic observation further proved that two concen...

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Abstract

The invention discloses a nanometer antibacterial agent for preventing and controlling plant fungal diseases. The nanometer antibacterial agent is composed of the suspending liquid formed by nanometercopper oxide and water, wherein the concentration of nanometer copper oxide is 50-200mg / L. The efficient plant antibacterial agent prepared according to the invention has obvious effects of promotingthe pathogen resistance of plants and restraining the fungus infection of plant blades and is capable of effectively preventing and restraining the fungus infection of plant blades.

Description

technical field [0001] The invention belongs to the technical field of plant protection, in particular to a high-efficiency plant antifungal agent. Specifically, it relates to a nanometer antibacterial agent used for preventing and controlling fungal infection of plants, including its main components, preparation method and application method. Background technique [0002] Plant fungal diseases have always been an important disease affecting agricultural production. At present, fungal diseases of plants have accounted for more than 70% of the total number of diseases worldwide, and are widely distributed in my country, such as: rot, anthracnose, ring spot, powdery mildew, black spot, dry rot, etc. The impact of fungal diseases on crops runs through all aspects of agricultural product production, product transportation, storage, and sales. The main harm is reflected in the reduction of crop yield and quality, and the impact on crop production safety. [0003] At present, th...

Claims

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Application Information

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IPC IPC(8): A01N59/20A01P3/00
CPCA01N59/20
Inventor 芮玉奎郝毅段成王瑶瑶
Owner CHINA AGRI UNIV
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