Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of metolachlor molecular imprinting sensor

A technology of metolachlor and molecular imprinting, which is applied to instruments, scientific instruments, analytical materials, etc., can solve problems affecting the application of molecular imprinting technology, slow electron transfer speed and response, and difficulty in controlling the thickness of imprinted membranes, achieving high Effects of affinity and selectivity, high sensitivity, and improved response

Inactive Publication Date: 2018-12-21
UNIV OF JINAN
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the thickness of the imprinted film prepared by the traditional imprinting method is difficult to control, and the high degree of cross-linking makes the electron transfer speed and response slow, and the lower limit of detection is high, which affects the application of molecular imprinting technology in electrochemical sensors.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1) Preparation of nano-gold / graphene oxide modification solution: In the reactor, add 88 mL of deionized water, chloroauric acid: 2.0 g, graphene oxide: 5.0 g, sonicate for 20 minutes at room temperature, disperse evenly, and then add Citric acid: 5.0g, heated to 55±2°C, stirred and reacted for 40min to obtain nano-gold / graphene oxide modification solution;

[0023] (2) Preparation of nano-gold / graphene oxide modified electrode: Polish the glassy carbon electrode with 0.3 μm and 0.01 μm polishing powder in turn, then ultrasonically clean it with twice distilled water, wash with ethanol, dry it, and soak it in the glassy carbon electrode In the nano-gold / graphene oxide modification solution for 30 minutes, take it out and place it under an infrared lamp, and evaporate the solvent to obtain a nano-gold / graphene oxide modified electrode;

[0024] (3) Preparation of metolachlor molecularly imprinted polymer: In the reactor, add ethanol: 98 mL, N-ethyl-2-aminomethylpyrrolidi...

Embodiment 2

[0027] 1) Preparation of nano-gold / graphene oxide modification solution: In the reactor, add 92 mL of deionized water, chloroauric acid: 1.0 g, graphene oxide: 3.0 g, sonicate for 20 minutes at room temperature, disperse evenly, and then add Citric acid: 4.0g, heated to 55±2°C, stirred and reacted for 40min to obtain nano-gold / graphene oxide modification solution;

[0028] (2) Preparation of nano-gold / graphene oxide modified electrode: Polish the glassy carbon electrode with 0.3 μm and 0.01 μm polishing powder in turn, then ultrasonically clean it with twice distilled water, wash with ethanol, dry it, and soak it in the glassy carbon electrode In the nano-gold / graphene oxide modification solution for 30 minutes, take it out and place it under an infrared lamp, and evaporate the solvent to obtain a nano-gold / graphene oxide modified electrode;

[0029](3) Preparation of metolachlor molecularly imprinted polymer: In the reactor, add ethanol: 101 mL, N-ethyl-2-aminomethylpyrrolidi...

Embodiment 3

[0032] 1) Preparation of nano-gold / graphene oxide modification solution: In the reactor, add 85 mL of deionized water, chloroauric acid: 3.0 g, graphene oxide: 6.0 g, sonicate for 20 minutes at room temperature, disperse evenly, and then add Citric acid: 6.0g, heated to 55±2°C, stirred and reacted for 40min to obtain nano-gold / graphene oxide modification solution;

[0033] (2) Preparation of nano-gold / graphene oxide modified electrode: Polish the glassy carbon electrode with 0.3 μm and 0.01 μm polishing powder in turn, then ultrasonically clean it with twice distilled water, wash with ethanol, dry it, and soak it in the glassy carbon electrode In the nano-gold / graphene oxide modification solution for 30 minutes, take it out and place it under an infrared lamp, and evaporate the solvent to obtain a nano-gold / graphene oxide modified electrode;

[0034] (3) Preparation of metolachlor molecularly imprinted polymer: In the reactor, add ethanol: 94 mL, N-ethyl-2-aminomethylpyrrolidi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses a preparation method of a metolachlor molecular imprinting sensor. The preparation method is characterized by comprising the following steps of adding the following componentsin percentage concentration by mass: 74-80% of ethanol, 6-10% of N-ethyl-2-aminomethyl pyrrolidone, 6-10% of citraconic acid, 2-4% of metolachlor, 0.5-1.5% of diisocyanate and 3-5% of ammonium persulfate, wherein the sum of the contents of the components is 100%; performing stirring and dissolving, introducing nitrogen to remove oxygen for 10 minutes, and in an oxygen-free atmosphere, carrying outa stirring reaction at 60 + / -2 DEG C for 10-12 hours, enabling the obtained product to be soaked in a mixed solution with ethanol and acetic acid at the volume ratio of 8:1 for 10 hours, performing washing for multiple times, and removing template molecules, and carrying out drying to obtain a metolachlor molecular imprinting polymer; and the sensor has high sensitivity and selectivity. The sensor has the advantages of being high in sensitivity, high in specificity, rapid in detection and capable of being repeatedly used.

Description

technical field [0001] The present invention relates to a preparation method of a molecularly imprinted sensor and the technical field of rapid detection application, in particular to a method for preparing a molecularly imprinted sensor for metolachlor, which is used to detect metolachlor in a sample. Background technique [0002] The pure product of metolachlor is a colorless liquid, its chemical name is 2-methyl-6-ethyl-N-(1-methyl-2-methoxyethyl)-N-chloroacetylaniline; The specific gravity (20°C) is 1.12, the boiling point is 100°C, and the flash point is 110-180°C. The solubility in water is 530mg / kg (20°C), miscible with most organic solvents, and the storage stability at room temperature is more than two years. Metolachlor 72% EC is a brownish-yellow liquid with good emulsifying properties, can be mixed with many herbicides, and has a storage stability of more than two years at room temperature. Metolachlor is used in upland crops, vegetable crops, orchards, and nur...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N27/30G01N27/416G01N27/48
CPCG01N27/308G01N27/416G01N27/48
Inventor 李慧芝李志英李冬梅
Owner UNIV OF JINAN
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com