Preparation method of morchella liquid strains

A liquid strain, Morchella technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems such as low growth activity of mycelium, large diameter of mycelium, clogging of equipment, etc., To achieve the effect of shortening the seed production cycle, more growth points, and low cost

Active Publication Date: 2018-12-25
四川省食用菌研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The technical problems to be solved by the present invention are: the growth vigor of the morel liquid spawn mycel

Method used

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  • Preparation method of morchella liquid strains
  • Preparation method of morchella liquid strains
  • Preparation method of morchella liquid strains

Examples

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preparation example Construction

[0030] The invention provides a kind of preparation method of hickory chick liquid bacterial classification, comprises the following steps:

[0031] a. Inoculate the female hickory chick species in the PDA solid medium, and cultivate it at a constant temperature of 22-25° C. for 5-10 days;

[0032] B, get the solid culture medium gained in step a, inoculate in the large square baffle shake flask that liquid culture medium is housed, cultivate on a shaker for 2 to 4 days, to obtain the shake flask morel liquid bacterial classification;

[0033] c. Add liquid culture medium in the liquid strain fermenter, after sterilization, inoculate the shake flask morel liquid strain obtained in step b, under aseptic conditions, cultivate at 24-28°C for 1-3 days to obtain Morchella liquid strain;

[0034] The liquid medium is composed of: by weight percentage, bran 4%-7%, cornstarch 1%-3%, yeast powder 0.1%-0.4%, glucose 1%-3%, peptone 0.2%-0.4% , MgSO 4 0.05%~0.15%, KH 2 PO 4 0.1%~0.1...

Embodiment 1

[0060] Embodiment 1 prepares hickory chick liquid bacterial classification with the inventive method

[0061] The specific operation steps are as follows:

[0062] a. Select the morel mother species test tube with more sclerotia distribution, avoid the original inoculation block, cut out the medium with an area of ​​3mm × 3mm with a sterile scalpel blade, and inoculate it on a 90 × 15mm plate cultured with PDA solid medium In the center of the dish, after the inoculation is completed, turn the plate culture dish upside down, and cultivate it in a constant temperature incubator at 25°C for 7 days until the mycelium is covered with the plate culture dish.

[0063] The preparation method of described PDA solid culture medium is as follows: in every 1000mL water, add potato 150g that is chopped into 1 centimeter square, filter with double-layer gauze after boiling 20min, abandon filter residue, add glucose 20g, agar 15g, stir evenly, under pressure 0.1MPa, 121°C, sterilize for 15...

Embodiment 2

[0069] Embodiment 2 prepares hickory chick liquid bacterial classification with the inventive method

[0070] The specific operation steps are as follows:

[0071] a. Select the morel mother species test tube with more sclerotia distribution, avoid the original inoculation block, use a sterile scalpel to cut out the culture medium with an area of ​​7mm×7mm and a thickness of 3mm, and inoculate it on a 90×15mm solid medium with PDA After the inoculation was completed, the flat culture dish was inverted and cultured in a constant temperature incubator at 25°C for 5 days until the mycelium covered the flat culture dish.

[0072] The preparation method of described PDA solid culture medium is as follows: in every 1000mL water, add potato 250g that is chopped into 1 centimeter square, filter with double-layer gauze after boiling 20min, abandon filter residue, add glucose 30g, agar 15g, stir evenly, under pressure 0.1MPa, 121°C, sterilize for 15 minutes, and control the thickness o...

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Abstract

The invention belongs to the technical field of morchella artificial culture, particularly relates to a preparation method of morchella liquid strains, and aims to solve the problems that morchella liquid strains prepared by an existing method are low in mycelium growth activity and large in mycelium diameter, and equipment blocking is caused. The preparation method includes the steps of a, inoculating morchella mother strains into a PDA solid culture medium, and culturing for 5-10 days under constant temperature of 22-25 DEG C; b, taking the solid culture medium, inoculating into a four-edgelarge flask with indentation loaded with a liquid culture medium, and culturing on a shaking table for 2-4 days; c, adding a liquid culture medium into a liquid strain fermentation tank, sterilizing,inoculating the flask morchella liquid strains into the liquid culture medium, and culturing under a sterile condition and the temperature of 24-28 DEG C for 1-3 days to obtain the morchella liquid strains. The preparation method is low in time consumption, short in production cycle, consistent in strain age, good in repeatability, small in flocculent mycelium pellet diameter, high in mycelium activity and suitable for factory application.

Description

technical field [0001] The invention belongs to the technical field of hickory chick artificial cultivation, and in particular relates to a preparation method of hickory chick liquid strain. Background technique [0002] Morchella deliciosa Fr. is a rare edible and medicinal fungus. Different varieties of Morchella deliciosa Fr. contain 7 kinds of essential amino acids, the total amount of amino acids is nearly 40%, and the fat content is less than 1%. The cellulose, mineral elements, etc. belong to high-quality protein food. More than 400 years ago, "Compendium of Materia Medica" has recorded that it is "sweet and cold in taste, flat in nature, non-toxic, beneficial to the stomach, resolving phlegm and regulating qi". Modern medicine has also found that Morchella can enhance the body's immunity, has certain killing ability against liver cancer, lung cancer, colon cancer, breast cancer and other tumor cells, and can resist fatigue, lower blood fat, and reduce the toxicity c...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 许瀛引甘炳成彭卫红王勇唐杰陈影刘理旭
Owner 四川省食用菌研究所
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