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Cotton immune system activator and application thereof

A technology of the immune system and cotton, applied in the direction of application, biocide, animal repellent, etc., to achieve the effect of enhancing resistance and improving resistance

Inactive Publication Date: 2019-01-01
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Cotton is rich in secondary metabolites, but the role of these secondary metabolites in the resistance of cotton to Verticillium wilt has been rarely reported

Method used

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  • Cotton immune system activator and application thereof
  • Cotton immune system activator and application thereof
  • Cotton immune system activator and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Cotton Seedling Cultivation and Indole Treatment

[0024] Accelerate the germination of cotton seeds until the radicle grows to about 1-2cm, and place them in the hydroponic nutrient solution. The formula of the hydroponic solution is (in 2L volume): 5ml 1M KNO 3 ; 5ml 1M Ca(NO 3 )2.4H 2 0; 3ml Fe salt mother liquor (Fe salt mother liquor formula is: Na 2 .EDTA, 37.3mg / L; Fe(SO4) 2 .7H 2 O, 28.7mg / L); 3ml trace element mother liquor (the formula of trace mother liquor is: KI, 0.83mg / L; H 3 BO 3 , 6.2mg / L; MnS O4.4H 2 O, 22.3mg / L; ZnSO 4 .7H 2 O, 8.6 mg / L; Na 2 MoO 4 .2H 2 O, 0.25mg / L; CuSO 4 .5H 2 O, 0.025mg / L; CoCl 2 .6H 2 O, 0.025mg / L); 2ml 1M MgSO 4 .7H 2 O; 1ml 1M KH 2 PO 4 ; 1ml 1M KOH. The nutrient solution was changed every 4 days, and the cotton seedlings were cultivated in a light culture room at a temperature of 28°C, with a light of 120 μEM-2S-1, 16 hours of light and 8 hours of darkness, until the cotton plant grew to two leaves...

Embodiment 2

[0026] Example 2 Inoculation identification after indole treatment

[0027] After 24 hours of indole solution treatment, the conventional Verticillium dahliae V991 was inoculated with a spore concentration of 10 5 cells / ml, inoculated by root dipping method, and the inoculation time was 1min. After inoculation, the cotton seedlings were transferred to sterile nutrient soil, and the indole solution of corresponding concentration was irrigated once. On the 12th day after inoculation, the incidence of Verticillium wilt of cotton seedlings was counted, and the method was based on the method reported by Xu et al. (Xu et al., 2012). 16 days after inoculation with Verticillium dahliae, the wilting, yellowing and shedding of the plants in the indole treatment group were significantly reduced compared with the control treatment, especially the cotton plants treated with 500 μM and 1 mM indole could significantly enhance the resistance of cotton plants to Verticillium dahliae. sex (se...

Embodiment 3

[0028] Example 3 Transcriptome analysis after indole treatment

[0029] A. Indole Treatment Transcriptome

[0030] With the method described in Example 1, cotton seedlings were treated with 500 μM indole solution for 1h, 3h, 6h, 12h, 20h, and after 24h, the root tissue was taken to extract total RNA. The total RNA extraction method refers to the improved isothiocyanate of Zhu et al. Guanidine (Zhu et al., 2005). In the experiment, the water treatment group was used as the control, and a total of 3 biological repetitions were set up. Cotton root RNA samples treated with indole solution for 24 hours and control sample RNA were sent to Main Company (Beijing) for whole-genome transcriptome sequencing analysis. The results of transcriptome analysis showed that, compared with the control samples, indole treatment significantly activated the expression of genes related to disease resistance in the cotton immune system. Significant changes occurred in the gene expression of cotton ...

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Abstract

The invention belongs to the technical field of cotton plant protection, and particularly relates to a cotton immune system activator and an application thereof. According to the invention, a mutual relationship between an exogenous activator and a cotton immune system is researched by means of a molecular biology, and the result shows that indole can be used as a cotton immune system activator. After an indole solution with a concentration of 200 [mu]M or above is externally applied, the resistance of cottons to verticillium wilt can be remarkably enhanced. The whole genome transcriptome is analyzed, and the result shows that the expression of genes related to disease resistance, salicylic acid synthesis and signal path in a cotton immune system is remarkably activated after treatment byusing indole. The cotton immune system activator can be developed into a novel reagent for preventing and controlling cotton verticillium wilt, cotton seedlings are treated by spraying or drip irrigation, and the resistance of cottons to the verticillium wilt can be improved.

Description

technical field [0001] The invention belongs to the technical field of cotton plant protection, and in particular relates to a cotton immune system activator and application thereof. The present invention finds that indole can be used as a cotton immune system activator. External application of indole can significantly activate the expression of genes related to disease resistance and salicylic acid synthesis and signaling pathways in the cotton immune system, thereby enhancing the resistance of cotton to Verticillium dahliae. Background technique [0002] Cotton plays an important role in our national economy. With the improvement of people's living standards, people's demand and quality of natural textiles are getting higher and higher. At the same time, with the rise of labor prices, the planting area of ​​cotton is also declining year by year. This serious imbalance between supply and demand makes it difficult to cultivate high-yield and high-quality Disease-resistant ...

Claims

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Application Information

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IPC IPC(8): A01G7/06A01N43/38A01P21/00
CPCA01G7/06A01N43/38
Inventor 朱龙付张献龙苗玉焕
Owner HUAZHONG AGRI UNIV
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