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A kind of porous bismuth nano microsphere and its preparation method and application

A technology of bismuth nano and microspheres, applied in the field of its preparation, porous bismuth nano microspheres, can solve problems such as long-term high-intensity light, heat shock, etc., to ensure biocompatibility, reduce toxic effects, and improve therapeutic effects Effect

Active Publication Date: 2021-03-09
SHANGHAI UNIV OF ENG SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a single photothermal treatment requires long-term high-intensity light, resulting in heat shock caused by too high temperature

Method used

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  • A kind of porous bismuth nano microsphere and its preparation method and application
  • A kind of porous bismuth nano microsphere and its preparation method and application
  • A kind of porous bismuth nano microsphere and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0056] In this embodiment, porous Bi nanospheres are prepared by the following method, which specifically includes the following steps:

[0057] 1. Disperse 100mg of molybdenum oxide (about 0.7mmol) and 0.4851g of bismuth nitrate pentahydrate (about 1mmol) in a mixed solution composed of 30mL of glycerin and 5mL of ethylene glycol, and then add 0.4998g of polyvinylpyrrolidone K-30 (PVP) , ultrasonically stirred evenly.

[0058] 2. Oil bath, reflux and stir at 200°C under normal pressure for 4 hours, then cool to room temperature, centrifuge at 4000rpm for 4min, remove Bi nano-solid spheres with larger particle size, take the upper layer solution, and centrifuge at 11000rpm for 20min, Take the precipitate. Disperse the precipitate in deionized water, sonicate for 30 minutes, and centrifuge at 11,000 rpm for 20 minutes; take the precipitate and disperse it in absolute ethanol, ultrasonicate for 30 minutes, and centrifuge at 11,000 rpm for 20 minutes; take the precipitate and re...

Embodiment 2

[0067] In this embodiment, the porous Bi nanospheres prepared in Example 1 are investigated by the CCK-8 kit on the viability of HeLa cells and Endothelial (endothelial cells), and the method is as follows:

[0068] 1. Cell culture:

[0069] Inoculate human cervical cancer cells HeLa or endothelial cells to a cell density of 2×10 4 / mL, 200 μL per well in a 96-well plate, placed at 37 °C and 5% CO 2 Concentration of cell culture incubator for 24h.

[0070] 2. Determination of cell viability:

[0071] After 24 hours of attachment, replace the complete medium containing porous Bi nanospheres (from Example 1) with a concentration of 0, 1.25, 2.5, 5, 10, 20, 50, 100, 200 μg / mL, and set 6 in each group. Duplicate wells; after 24 hours of treatment, remove the medium; add 200 μL of CCK solution (diluted with serum-free medium at 1:20) to each well, incubate in a 37°C incubator for 1 hour, and measure the concentration at 450 nm on a microplate reader. For the absorbance value, t...

Embodiment 3

[0074] In the present embodiment, the radiotherapy effect of porous bismuth nano-microspheres is investigated, and the specific methods are as follows:

[0075] 1. Cell culture:

[0076] Inoculate human cervical cancer cells HeLa to a cell density of 2×10 5 Place the cells / well in a 60 mm diameter glass bottom dish at 37 °C and 5% CO 2 Concentration of cell culture incubator for 24h.

[0077] 2. Determination of cell viability:

[0078] After receiving different doses (0 μg / mL, 200 μg / mL) of pBi nanospheres (from Example 1) for incubation, they were treated with different doses (0, 2, 4, 6, 8 Gy) of X-ray irradiation to measure cell survival Measurement results such as Figure 6 As shown, different doses of X-ray irradiation have a significant effect on the viability of HeLa cells. When the same dose of X-rays is irradiated, porous bismuth nanospheres (pBi) significantly enhance the inhibitory effect of X-rays on HeLa cell viability, indicating that porous bismuth nanosphe...

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Abstract

The invention belongs to the field of multifunctional nanomaterials and particularly relates to porous bismuth nano-microspheres as well as a preparation method and an application thereof. A hydrophilic surface modifier is attached to the surfaces of the porous bismuth nano-microspheres, and three-dimensional through multi-pore structures are distributed in the microspheres. The preparation methodof the porous bismuth nano-microspheres comprises steps as follows: a molybdenum oxide template, bismuth salt and the hydrophilic surface modifier are uniformly dispersed in a mixed solution of glycerin and other alcohol solvents, a reflux reaction is performed, and the generated solid bismuth nano-microspheres are attached to the surface of the molybdenum oxide template; after the molybdenum oxide template is removed by alkali liquor, a solvothermal reaction is performed in a dimethyl sulfoxide solution, and the porous bismuth nano-microspheres are produced. The prepared porous bismuth nano-microspheres have radiopotentiation activity, photothermal conversion characteristic, drug loading activity and biocompatibility, achieve the synergistic therapeutic effect of radiotherapy, photothermic therapy and drug therapy, and have the application prospect of serving as a tumor diagnosis and treatment agent.

Description

technical field [0001] The invention belongs to the field of multifunctional nanomaterials, and in particular relates to a porous bismuth nanosphere, its preparation method and application. Background technique [0002] In the past few decades, human beings have made rapid progress in understanding and overcoming cancer, but cancer is still one of the major diseases that threaten human health and life expectancy. According to the "Global Cancer Report 2014" released by the World Health Organization: the world is facing a major cancer outbreak, and China has the highest incidence rate. The National Cancer Registry Center pointed out that in 2012, there were more than 3 million new cancer patients in my country, and about 2.5 million people died of cancer every year. Such high morbidity and mortality are largely due to the ineffectiveness of existing methods for cancer treatment , Toxic and side effects are large. [0003] Radiotherapy (RT) is a new method of treating tumor d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K47/02A61K47/32A61K41/00A61K51/02A61K51/06A61K51/12A61K31/704A61P35/00
CPCA61K9/5115A61K9/5138A61K31/704A61K41/0052A61K41/0057A61K51/02A61K51/06A61K51/1251A61P35/00A61K2300/00
Inventor 马国昌刘锡建袁海宽陆杰
Owner SHANGHAI UNIV OF ENG SCI
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