Preparation method and application of pH-responsive dual-drug-release metal-organic framework-upconversion nano-system

A metal-organic framework, dual-drug technology, applied in pharmaceutical formulations, drug combinations, drug delivery, etc., can solve the problems of large structure size, low drug loading, small specific surface area, etc., to reduce the forming size, simplify the preparation process, Efficient effect of the preparation process

Inactive Publication Date: 2019-01-11
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, it is generally impossible to combine MOFs with upconversion nanoparticles using traditional components and preparation methods. At the same time, the traditional preparation method has a strong singleness, and cannot effectively use chemical bonds and physical adsorption to form a dual drug-loaded system. Treatment improves efficacy
[0006] The existing inorganic mesoporous materials, organic macromolecular materials, liposomes, and biodegradable polymer materials used as drug carriers are relatively complex composite systems, which are difficult to achieve nanoscale structures by conventional methods, and Traditional composite system materials have large structural size, poor dispersion and stability, small specific surface area, no highly ordered porous structure, unadjustable structural components, uncontrollable size and shape, poor biocompatibility and drug resistance. The loading capacity is low, and it cannot have the characteristics of near-infrared fluorescence imaging and magnetic resonance imaging (MRI) dual-mode imaging probes at the same time, and it is impossible to realize the combination of visual tracers and MOFs to form a composite material system integrating diagnosis and treatment.

Method used

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  • Preparation method and application of pH-responsive dual-drug-release metal-organic framework-upconversion nano-system
  • Preparation method and application of pH-responsive dual-drug-release metal-organic framework-upconversion nano-system
  • Preparation method and application of pH-responsive dual-drug-release metal-organic framework-upconversion nano-system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] See Figure 1-2 , this example provides a pH-responsive dual drug release metal-organic framework and surface coated with inert NaGdF 4 The preparation method of the up-conversion nano system doped with layer rare earth erbium specifically comprises the following steps:

[0035] 1) Prepare 10 mg of oil-soluble NaYF with a particle size of 30-35 nm 4 :Yb,Er@NaGdF 4 Disperse in cyclohexane, add 10mL of water, then add a small amount of dilute hydrochloric acid to adjust the pH to 1-2 and react at room temperature for 3-5 hours to remove the oleic acid ligand. After the reaction, transfer the mixed solution to a centrifuge In the tube, centrifuge at 12 000rpm for 10min to take the solid; wash with water and ethanol respectively, and finally disperse in 10mL of water to obtain ligand-removed upconverting nanoparticles (UCNPs);

[0036] 2) Add 25mg of polyvinylpyrrolidone (PVP), keep stirring at 30°C for 12h, fully stir and dissolve, keep stirring at 30°C, and react for 1...

Embodiment 2

[0046] This example provides and surface coated with inert NaGdF 4 The preparation method of the up-conversion nano system doped with rare earth erbium is basically the same as that of Example 1, except that it comprises the following steps:

[0047] 1) Prepare 10 mg of oil-soluble NaYF with a particle size of 30-35 nm 4 :Yb,Er@NaGdF 4 Disperse in cyclohexane, add 10mL of water, then add a small amount of dilute hydrochloric acid to adjust the pH to 1-2 and react at room temperature for 3-5 hours to remove the oleic acid ligand. After the reaction, transfer the mixed solution to a centrifuge In the tube, centrifuge at 12 000rpm for 10min to take the solid; wash with water and ethanol respectively, and finally disperse in 10mL of water to obtain ligand-removed upconverting nanoparticles (UCNPs);

[0048] 2) Add 25mg of polyvinylpyrrolidone (PVP), keep stirring at 30°C for 12h, fully stir and dissolve, keep stirring at 30°C, and react for 12h. The above reaction mixture solut...

Embodiment 3

[0053] This example provides a pH-responsive dual drug release metal-organic framework and surface coated with inert NaGdF 4 The preparation method of the up-conversion nano system doped with rare earth erbium is basically the same as that of Example 1-2, except that it comprises the following steps:

[0054] 1) Prepare 10 mg of oil-soluble NaYF with a particle size of 30-35 nm 4 :Yb,Er@NaGdF 4 Disperse in cyclohexane, add 10mL of water, then add a small amount of dilute hydrochloric acid to adjust the pH to 1-2 and react at room temperature for 3-5 hours to remove the oleic acid ligand. After the reaction, transfer the mixed solution to a centrifuge In the tube, centrifuge at 12 000rpm for 10min to take the solid; wash with water and ethanol respectively, and finally disperse in 10mL of water to obtain ligand-removed upconverting nanoparticles (UCNPs);

[0055] 2) Add 25mg of polyvinylpyrrolidone (PVP), keep stirring at 30°C for 12h, fully stir and dissolve, keep stirring a...

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Abstract

The invention discloses a preparation method of a pH-responsive dual-drug-release metal-organic framework-upconversion nano-system. The preparation method comprises the following steps of: adopting apolymer modification method to improve the water solubility of the upconversion nano-crystal with a core-shell structure, and growing a metal-organic framework on the surface of the upconversion luminescent nano-crystal to obtain an upconversion nano-system coated with the metal-organic framework; and attaching Adriamycin hydrochloride containing amino group to the surface of the metal-organic framework, and adsorbing other anticancer drugs into the porous structure of the metal-organic framework to obtain the double-drug-loaded metal-organic framework-up-conversion nano-system. The nano system provided by the invention has good stability, good biocompatibility, simple and reliable preparation method and good repeatability. The nanosystems have tumor diagnostics-Therapeutic integration canbe used as an imaging agent for fluorescence imaging and magnetic resonance imaging, as well as drug delivery and pH-responsive dual drug release, and can be used as a drug carrier.

Description

technical field [0001] The invention relates to the technical field of nano-biological materials, in particular to a preparation method and application of a pH-responsive double-drug release metal-organic framework-upconversion nano system. Background technique [0002] As a classic functional material, metal-organic frameworks (MOFs) materials have become one of the most exciting, diverse, and fastest-growing fields of modern chemical research. MOFs are composed of metal ions or ion clusters linked to organic Ligands form a series of composite materials with two-dimensional or three-dimensional periodic structures. MOFs have the advantages of both inorganic materials and organic materials, such as large specific surface area, highly ordered porous structure, adjustable structural components, controllable size and shape, and good biocompatibility. These advantages enable MOFs-based materials not only to be used in gas storage, separation, catalysis, etc., but also to be use...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61K47/69A61K49/18A61K49/00A61P35/00A61K31/513
CPCA61K31/513A61K31/704A61K49/0002A61K49/0093A61K49/183A61K47/6929A61P35/00A61K2300/00
Inventor 孙丽宁凌丹萍李海宏施利毅赵慧君
Owner SHANGHAI UNIV
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