Bacillus siamensis and application thereof in degradation of dibutyl phthalate
A technology of dibutyl phthalate and Bacillus, applied in the fields of microbiology and food safety, to achieve the effect of degrading residual pollution and reducing absorption and translocation
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Embodiment 1
[0026] Example 1 Isolation, identification and degradation function verification of Bacillus siamese T7
[0027] 1. Strains Bacillus siamensis sp.T7 isolation and identification
[0028] In July 2016, the applicant collected the wild weed sorrel from the experimental field of Jiangsu Academy of Agricultural Sciences ((E118°86′, N32°03′) in the plastic greenhouse, and then sterilized the surface of the sorrel plant and ground it into juice , coated on an inorganic salt solid medium containing DBP as the only carbon and nitrogen source, and screened to obtain a plant endophyte with dibutyl phthalate degradation characteristics Bacillus siamensis sp. T7.
[0029] The applicant self-named the bacterium as T7, and its electron microscope pictures are as follows: figure 1 As shown, the strain is rod-shaped, and polysaccharides are secreted on the surface, so that individual strains can adhere to each other, and when the amount of bacteria is large, it forms a biofilm and exist...
Embodiment 2
[0035] Example 2 Preparation of rice husk biochar
[0036] The biomass waste rice husk after rice threshing is selected, exposed to the sun and air-dried for a week under sunny conditions, and then dried in an oven at 120 degrees Celsius for 24 hours to achieve complete dehydration; then put the rice husk biomass into the SG-GL1100K high-temperature furnace, 500 degrees Celsius The biochar raw material was obtained by cracking for four hours; finally, the biochar raw material was ground and screened to obtain rice husk biochar with a particle size of 0.154 mm, which could be used as a microbial loading agent for the T7 strain.
[0037] The ASAP2020 automatic specific surface area and pore distribution instrument and the 2400II elemental analyzer were used to analyze the rice husk biochar obtained in this example, and the characteristics of its surface functional groups were determined by the chemical indicator method. The test results are shown in Table 1:
[0038] Table 1 Tes...
Embodiment 3
[0041] Embodiment 3 DBP soil remediation agent preparation
[0042] The rice husk biochar prepared in Example 2 was put into an Erlenmeyer flask, and sterilized by high pressure steam at 121 degrees Celsius for 20 minutes; Mixing, every 1 gram of biochar (dry weight) is mixed with 200 ml of bacterial suspension, and at the same time, 0.01 mol per liter (bacterial liquid) of calcium chloride and 0.2 gram per liter of sodium azide are added, and then the rice husk is raw The mixture of material charcoal and bacterial suspension was placed in a shaker at 30 degrees Celsius, and fixed by physical adsorption at low speed (80 rpm) for 24 hours; then the bacterial liquid was filtered through a 200-mesh sieve, and the microbial loading agent was rinsed three times with sterile PBS , in order to remove the T7 bacteria that are not adsorbed on the surface of the biochar, and finally place the rice husk biochar loaded with T7 bacteria in an oven at 35 degrees Celsius and dry for 24 hours...
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