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Method for producing bacterial protein by treating fatty acid wastewater with photosynthetic bacteria

A technology of photosynthetic bacteria and bacterial protein, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problem of long treatment time of fatty acid wastewater

Active Publication Date: 2021-07-06
SHENYANG POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These three methods all take a long time to treat fatty acid wastewater

Method used

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  • Method for producing bacterial protein by treating fatty acid wastewater with photosynthetic bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The photosynthetic bacteria process the fatty acid wastewater to produce the cell protein method, comprising the following steps:

[0023] (1) Domestication of photosynthetic bacteria: select domesticated halophilic photosynthetic bacteria liquid;

[0024] (2) Wastewater treatment: the sulfate content of fatty acid production wastewater is 35%, dilute 1L of fatty acid wastewater by 10 times, add 1% nutrient salt solution of treated water volume, the nutrient salt solution is composed of EDTA (0.5g / L), CaCl 2 ·H 2 O (0.5g / L), ZnSO 4 ·7H 2 O (0.05g / L), FeSO 4 ·7H 2 O (0.9g / L), KCl (0.2g / L), CoCl 2 ·6H 2 O (0.09g / L), MgCl 2 ·6H 2 O (0.6g / L); Control the pH value of waste water with potassium carbonate at 8, inoculate photosynthetic bacteria, at 30 ℃ of culture temperature, under the irradiation of 40W incandescent lamp, cultivate after 3 days, add magnesium chloride solution 0.001g according to every liter of waste water, The bacterial protein is separated, and t...

Embodiment 2

[0027] The photosynthetic bacteria process the fatty acid wastewater to produce the cell protein method, comprising the following steps:

[0028] (1) Domestication of photosynthetic bacteria: select the mixed bacterial solution of domesticated photosynthetic bacteria and domesticated halophilic photosynthetic bacteria,

[0029] (2) Wastewater treatment: the sulfate content of fatty acid production wastewater is 30%, dilute 1L of fatty acid wastewater by 5 times, add 4% nutrient salt solution of treated water volume, and the nutrient salt solution is composed of EDTA (1.3g / L), CaCl 2 ·H 2 O (0.05g / L), ZnSO 4 ·7H 2 O (0.50g / L), FeSO 4 ·7H 2 O (0.2g / L), KCl (0.8g / L), CoCl 2 ·6H 2 O (0.01g / L), MgCl 2 ·6H 2 O (1.9g / L); use calcium carbonate to control the pH value of wastewater at 7.5, inoculate photosynthetic bacteria, and cultivate at a temperature of 30°C under sunlight (all sunny days) for 5 days, then add zinc sulfate per liter of wastewater 0.002 g of the solution, ...

Embodiment 3

[0032] The photosynthetic bacteria process the fatty acid wastewater to produce the cell protein method, comprising the following steps:

[0033] (1) Domestication of photosynthetic bacteria: select domesticated halophilic photosynthetic bacteria liquid;

[0034] (2) Wastewater treatment: the sulfate content of fatty acid production wastewater is 25%, dilute 1L of fatty acid wastewater by 2 times, and add 5% nutrient salt solution of treated water volume, the nutrient salt solution is composed of EDTA (0.8 / L), CaCl 2 ·H 2 O (0.2g / L), ZnSO 4 ·7H 2 O (0.3g / L), FeSO 4 ·7H 2 O (0.7g / L), KCl (0.6g / L), CoCl 2 ·6H 2 O (0.05g / L), MgCl 2 ·6H 2O (0.95g / L); Control the pH value of waste water with sodium carbonate at 7.5, inoculate photosynthetic bacteria, at 20 ℃ of cultivation temperature, under the irradiation of 40W fluorescent lamp, cultivate after 5 days, add aluminum sulfate solution 0.005g according to every liter of waste water, The bacterial protein is separated, and t...

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Abstract

The invention relates to a method for photosynthetic bacteria to treat fatty acid wastewater to produce bacterial protein. The method includes the following steps: domestication of photosynthetic bacteria; wastewater treatment: the sulfate content of fatty acid production wastewater is 20% to 35%, and the fatty acid wastewater is diluted for 2~ 10 times, add 1%~10% nutrient salt solution of the treated water volume, the nutrient salt solution is composed of EDTA, CaCl 2 ·H 2 O, ZnSO 4 ·7H 2 O, FeSO 4 ·7H 2 O, KCl, CoCl 2 ·6H 2 O, MgC1 2 ·6H 2 O; control the pH value of the wastewater at 7~8, inoculate photosynthetic bacteria, culture at a culture temperature of 20~35°C, and cultivate under light conditions for 3~10 days, add 0.001~0.005g of precipitant per liter of wastewater, and separate bacterial protein , drying the cell protein to obtain a dry product. The invention effectively avoids the disadvantage of obtaining miscellaneous salts after the treatment of salty wastewater, simplifies the treatment process of fatty acid wastewater, realizes the harmlessness and recycling of fatty acid wastewater, and can obtain high-protein feed at the same time without secondary pollution.

Description

technical field [0001] The invention relates to wastewater treatment technology in the field of environmental protection, in particular to a method for photosynthetic bacteria to treat fatty acid wastewater and produce cell protein. Background technique [0002] Fatty acid wastewater refers to the wastewater produced after soybean oil horns are saponified with sodium hydroxide, acidified with sulfuric acid, and separated from fatty acids. The wastewater has the characteristics of high sulfate, high acidity, high color and high COD. In the past scientific research projects, it is not uncommon to treat fatty acid wastewater with ecological technology, but there is no report on the direct use of photosynthetic bacteria to treat fatty acid production wastewater. Zhang Guobing, Sui Haibo's invention patent (patent application number: 201320489473.3) discloses a treatment system for fatty acid wastewater. The tower, sedimentation tank, aerobic aeration tank, sedimentation discha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34C12N1/20C12R1/01C02F101/10
CPCC02F3/34C02F3/345C02F2101/101C12N1/20
Inventor 郝天天李良于金龙刘馨芷姚瑶徐畅田太福张健
Owner SHENYANG POLYTECHNIC UNIV
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