A gene encoding β-glucosidase and its expression vector and protein

Abstract

The invention relates to a gene encoding beta-glucosidase, at least containing a DNA sheet containing one of that following nucleotide sequence: 1) a nucleotide sequence of SEQ ID NO. 1 in a sequencetable; 2) a nucleotide sequence have more than 90% homology with that nucleotide sequence shown in SEQ ID NO. 1 and encoding the same biological function protein; 3) a nucleotide sequence hybridize tothat nucleotide sequence shown in SEQ ID NO. 1 and encoding a protein of the same biological function. The recombinant vector further constructed according to the gene sequence of the invention can realize beta-glucosidase soluble expression, and simple affinity purification, can be obtained more than 95% purity and higher concentration of the active protein to maintain natural conformation. Thisis conducive to the industrial production of beta-Glucosidase.

Description

technical field

[0001] The invention belongs to the technical field of biomolecular cloning, and relates to a gene encoding beta-glucosidase, an expression carrier and protein thereof. Background technique

[0002] β-glucosidase (β-D-Glucosidase, EC3.2.1.21), also known as β-D-glucoside glucohydrolase, alias gentiobiase, cellobiase (cellobias, CB or β-G ) and amygdalinase. It belongs to cellulase and is an important component of cellulolytic enzyme system. It can hydrolyze the non-reducing β-D-glucose bond bound to the terminal and release β-D-glucose and corresponding ligands at the same time. β-glucosidase also has transglycosidase activity, the purpose is to synthesize functional oligoglucans, oligomeric maltooligosaccharides, oligomeric cellulooligosaccharides, etc. through the glucosidase with transglycosidase activity, which can be used as functional prebiotics carbohydrate. Therefore, the protein has important application value in food, feeding and health products ...

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