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Method for preparing N-acetvlneuraminic acid through integrated polysialic acid separation and purification

A technology of acetylneuraminic acid and polysialic acid, which is applied in the preparation of sugar derivatives, chemical instruments and methods, sugar compounds with non-glycosyl groups, etc., can solve problems such as high price, achieve low cost, simplify production steps, The effect of reducing production costs

Active Publication Date: 2019-01-25
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, market demand areas such as food, health care and medicine have higher quality requirements for N-acetylneuraminic acid products, and the purity requirements are greater than 98%. However, products with a purity greater than 98% are very expensive on the market

Method used

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  • Method for preparing N-acetvlneuraminic acid through integrated polysialic acid separation and purification
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  • Method for preparing N-acetvlneuraminic acid through integrated polysialic acid separation and purification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The method for the separation and purification of polysialic acid integrated in this example to prepare N-acetylneuraminic acid is as follows:

[0029] 1. Pretreatment of polysialic feed liquid: polysialic acid derived from microbial fermentation is used as raw material, and the feed liquid containing polysialic acid is obtained through membrane filtration and sterilization steps;

[0030] 2. Hydrolysis of polysialic acid: add 0.1M oxalic acid aqueous solution to the pretreated fermentation broth containing polysialic acid, heat the temperature to 80°C, and heat for 4 hours to hydrolyze polysialic acid into N-acetylneuramine acid;

[0031] 3. Feed liquid neutralization: Add calcium hydroxide aqueous solution to the above hydrolyzed aqueous solution, and neutralize the pH of the solution to 7.0.

[0032] 4. Precipitate protein and salt: Add 2 times the volume of ethanol to the above neutralized solution to precipitate impurity proteins and some salt impurities that are ...

Embodiment 2

[0036] The method for the separation and purification of polysialic acid integrated in this example to prepare N-acetylneuraminic acid is as follows:

[0037] 1. Pretreatment of polysialic feed liquid: polysialic acid derived from microbial fermentation is used as raw material, and the feed liquid containing polysialic acid is obtained through membrane filtration and sterilization steps;

[0038] 2. Hydrolysis of polysialic acid: Add 0.5M oxalic acid aqueous solution to the pretreated fermentation broth containing polysialic acid, heat the temperature to 80°C for 4 hours, and hydrolyze polysialic acid into N-acetylneuramine acid;

[0039] 3. Feed liquid neutralization: Add calcium bicarbonate aqueous solution to the above hydrolyzed aqueous solution, and neutralize the pH of the solution to 7.0.

[0040] 4. Precipitate protein and salt: Add 3 times the volume of ethanol to the above neutralized solution to precipitate impurity protein and some salt impurities insoluble in eth...

Embodiment 3

[0044] The method for the separation and purification of polysialic acid integrated in this example to prepare N-acetylneuraminic acid is as follows:

[0045] 1. Pretreatment of polysialic feed liquid: polysialic acid derived from microbial fermentation is used as raw material, and the feed liquid containing polysialic acid is obtained through membrane filtration and sterilization steps;

[0046] 2. Hydrolysis of polysialic acid: add 0.1M oxalic acid aqueous solution to the pretreated fermentation broth containing polysialic acid, heat the temperature to 70°C for 5 hours, and hydrolyze polysialic acid into N-acetylneuramine acid;

[0047] 3. Feed liquid neutralization: Add calcium hydroxide aqueous solution to the above hydrolyzed aqueous solution, and neutralize the pH of the solution to 7.0.

[0048] 4. Precipitate protein and salt: Add 4 times the volume of ethanol to the above neutralized solution to precipitate impurity proteins and some salt impurities that are insolubl...

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Abstract

The invention discloses a method for preparing N-acetvlneuraminic acid through integrated polysialic acid separation and purification. The method comprises the steps that feed liquid containing the polysialic acid serves as a raw material and is hydrolyzed with oxalic acid, calcium, magnesium and other ions are precipitated with oxalic acid, protein impurities are precipitated with ethyl alcohol,activated carbon decoloration is conducted, and precipitates, such as calcium salt and magnesium salt and impurities, such as proteins, are removed through filtration of an activated carbon carrier; the high-purity N-acetvlneuraminic acid is obtained through clear liquid concentration, acidification, crystallization and drying. By testing, the purity of the N-acetvlneuraminic acid is at least 98%,and the N-acetvlneuraminic acid can meet requirements of the fields, such as foods, health care, medicines and cosmetics. The method is simple, easy to operate and especially suitable for productionof the N-acetvlneuraminic acid through industrial fermentation.

Description

technical field [0001] The invention relates to an integrated polysialic acid separation and purification method for preparing N-acetylneuraminic acid, which belongs to the technical field of biochemical separation engineering. Background technique [0002] Sialic acid is a family of neuraminic acid derivatives. In recent years, the application of sialic acid and its derivatives in food, health products and medicine has increasingly broad development prospects. etc. It plays a very important role, especially in the application prospect of infant formula milk powder. [0003] The preparation methods of sialic acid include enzymatic catalysis, chemical synthesis and natural product extraction. But each of these methods has disadvantages. The microbial fermentation method developed in recent years is a hot spot that people pay attention to. Its advantage is high yield, but its disadvantage is complicated separation and purification. In the prior art, the cells are generally ...

Claims

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Application Information

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IPC IPC(8): C07H7/033C07H1/00C07H1/06
CPCC07H1/00C07H1/06C07H7/033
Inventor 袁丽霞吴志波陈祥松吴金勇孙立洁朱薇薇李翔宇王纪姚建铭
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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