Method for preparing N-acetylneuraminic acid hydrate by adjusting hydrogen ion concentration of solution

A technology of acetylneuraminic acid and hydrogen ions, which is applied in the field of preparing N-acetylneuraminic acid hydrate, can solve the problem of high crystallization cost, and achieve the effects of shortened crystallization time, short crystallization time and low environmental pressure

Active Publication Date: 2019-02-01
武汉中科光谷绿色生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In order to solve the problem of high crystallization cost of N-acetylneuraminic acid glacial acetic acid produced by microbial fermentation, the present invention aims to provide a method for prepari

Method used

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  • Method for preparing N-acetylneuraminic acid hydrate by adjusting hydrogen ion concentration of solution
  • Method for preparing N-acetylneuraminic acid hydrate by adjusting hydrogen ion concentration of solution

Examples

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Embodiment 1

[0022] In this example, the method for preparing N-acetylneuraminic acid hydrate by adjusting the concentration of hydrogen ions in the solution is as follows:

[0023] 1. N-acetylneuraminic acid microbial fermentation broth, after being roughly extracted by sterilization, protein removal, salt removal, decolorization, etc., concentrated to 300g / l, ready to use;

[0024] 2. Add concentrated hydrochloric acid to the concentrated solution of N-acetylneuraminic acid obtained in step 1 to adjust the hydrogen ion concentration of the system to pH 1.0;

[0025] 3. Put the mixed liquid obtained in step 1 to crystallize at a low temperature of 10°C until the crystals are precipitated. After the precipitation, let the crystals mature for 12 hours, wash with pure water, the weight ratio of crystals to pure water is 1:1, and dry at 60°C to obtain N-acetylneuraminic acid dihydrate.

[0026] Detected by high performance liquid chromatography, the content of N-acetylneuraminic acid is 89.2...

Embodiment 2

[0028] In this example, the method for preparing N-acetylneuraminic acid hydrate by adjusting the concentration of hydrogen ions is as follows:

[0029] 1. N-acetylneuraminic acid microbial fermentation broth, after being roughly extracted by sterilization, protein removal, salt removal, decolorization, etc., concentrated to 400g / l, ready to use;

[0030] 2. Add concentrated hydrochloric acid to the N-acetylneuraminic acid aqueous solution obtained in step 1, and adjust the hydrogen ion concentration of the system to pH 1.5;

[0031] 3. Put the mixed liquid obtained in step 1 to crystallize at a low temperature of 4°C until the crystals are precipitated. After the precipitation, let the crystals mature for 24 hours, wash with ethanol, the crystal weight to ethanol weight ratio is 1:1, and dry at 80°C to obtain N- Acetylneuraminic acid dihydrate.

[0032] Detected by high performance liquid chromatography, the content of N-acetylneuraminic acid was 89.0%, the calculated purity...

Embodiment 3

[0034] This implementation is according to embodiment 2 steps, except that the concentrated solution concentration of step 1 is 40g / l, other are all with embodiment 2 steps, the N-acetylneuraminic acid concentrated solution produced by microbial fermentation method is carried out strong acid pH adjustment, finally N-acetylneuraminic acid dihydrate did not crystallize.

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Abstract

The invention discloses a method for preparing N-acetylneuraminic acid hydrate by adjusting hydrogen ion concentration of a solution, comprising the following steps: firstly adding an acid solution toan aqueous solution of N-acetylneuraminic acid to adjust hydrogen ion concentration (namely pH value) of a solution system; crystallizing the obtained mixed liquor by standing at low temperature, washing crystals and drying to obtain the N-acetylneuraminic acid hydrate. Through testing, the purity of the N-acetylneuraminic acid hydrate is at least 99%, which can make the product meet the requirements in the fields of food, health care, medicine and cosmetics. In addition, the method is simple and easy to operate, and is especially suitable for industrial production of the N-acetylneuraminic acid hydrate.

Description

technical field [0001] The invention relates to a method for preparing N-acetylneuraminic acid hydrate by adjusting the concentration of hydrogen ions in a solution, and belongs to the technical field of biochemical engineering. Background technique [0002] N-acetylneuraminic acid, also known as sialic acid and bird's nest acid, has the effects of improving intelligence, memory and anti-senile dementia. N-acetylneuraminic acid derived from microbial fermentation has been approved as a new food raw material by the National Health and Family Planning Commission. The main steps of separation and purification of N-acetylneuraminic acid fermentation liquid prepared by microbial fermentation are sterilization, protein removal, hydrolysis, decolorization, desalination, impurity removal and concentration. Wash with absolute ethanol. During the crystallization process, a large amount of glacial acetic acid (7 to 9 times the volume of the concentrated solution) was used, and then a...

Claims

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Application Information

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IPC IPC(8): C07D309/14
CPCC07D309/14
Inventor 袁丽霞吴金勇陈祥松孙立洁李翔宇姚建铭朱薇薇王纪王刚王煜费贤春
Owner 武汉中科光谷绿色生物技术有限公司
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