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M-CDs for quickly detecting content of NO2<-> in food and environment and application method of m-CDs

A carbon quantum dot and nitrite technology, applied in analytical chemistry and nanometer fields, can solve the problems of complicated operation, high instrument cost, low sensitivity, etc., and achieve the effects of low cost, simple operation and wide application range.

Active Publication Date: 2019-02-15
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For existing NO 2 - The detection method has problems such as high instrument cost, complicated operation, and low sensitivity. The present invention provides a carbon quantum dot and its application method for rapidly detecting nitrite content in food and the environment. Advantages of simplicity and fluorescence visualization semi-quantitative analysis

Method used

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  • M-CDs for quickly detecting content of NO2&lt;-&gt; in food and environment and application method of m-CDs
  • M-CDs for quickly detecting content of NO2&lt;-&gt; in food and environment and application method of m-CDs
  • M-CDs for quickly detecting content of NO2&lt;-&gt; in food and environment and application method of m-CDs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: carbon quantum dots m -Synthesis of CDs

[0023] Dissolve m-phenylenediamine (0.60 g) in absolute ethanol (60 mL) and sonicate for 5 min to homogenize it. The mixed solution was transferred to a 100 mL polytetrafluoroethylene-lined autoclave, reacted at 180 °C for 12 hours, and then naturally cooled to room temperature to obtain a brown solution. Using silica gel column chromatography, the mixed solution of dichloromethane and methanol (15:1, v / v) was used as the eluent for further separation and purification, and the fluorescent carbon quantum dots were collected.

[0024] figure 1 for income m -The relevant characterization spectrum of CDs, wherein, Figure A is m - Transmission electron microscope image of CDs, Figure B is m -The particle size distribution diagram of CDs; Figure C is m -Atomic force microscopy images of CDs; Figure D is the corresponding image of Figure C m -Height of CDs; Figure E is m-phenylenediamine and m -Infrared contrast i...

Embodiment 2

[0025] Example 2: NO 2 - Drawing of standard curve

[0026] Preparation of NO with ultrapure water 2 - Solution, at room temperature, to 20 μg / mL m - CDs sulfuric acid solution (0.1 M) was added sequentially with different concentrations of NO 2 - solution (NO 2 - The final concentrations are 0, 0.063, 0.125, 0.25, 0.5, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0 μM), and the mixed reaction was determined after 60 min m -CDs fluorescence intensity changes and plotted about m -CDs fluorescence in response to NO 2 - standard curve.

[0027] figure 2 for m -CDs respond to NO 2 - The change graph of fluorescence intensity (A) and the drawn standard curve (B). It can be seen from the figure that the response range is 0.063-6.0 μM, and the detection limit LOD is 0.018 μM.

Embodiment 3

[0028] Example 3: NO in milk powder and luncheon meat quality control samples 2 - Content detection

[0029] (1) Sample extraction: Extract NO in quality control samples milk powder and luncheon meat according to Chinese national standard (GB 5009.33-2010) 2 -, the specific operation steps are: Weigh 5 g (accurate to 0.01 g) of a homogenized sample (if water is added during the preparation process, it should be converted according to the amount of water added), put it in a 50 mL beaker, add 12.5 mL of saturated borax solution , stir evenly, wash the sample into a 500 mL volumetric flask with about 300 mL of water at about 70 °C, heat in a boiling water bath for 15 min, take it out, put it in a cold water bath to cool, and place it at room temperature; when shaking the obtained extract Add 5 mL of potassium ferrocyanide solution, shake well, then add 5 mL of zinc acetate solution to precipitate protein; add water to the mark, shake well, let stand for 30 min, remove the uppe...

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Abstract

The invention discloses m-CDs for quickly detecting content of NO2<-> in food and environment and an application method of the m-CDs. The m-CDs are obtained through solvothermal method synthesis, separation and purification with m-phenylenediamine as a raw material; the m-CDs can be subjected to a diazotization reaction with NO2<-> specifically under a strong acid condition and have fluorescence quenching, so that the content of the NO2<-> can be detected according to change of fluorescence intensity of the m-CDs. Meanwhile, according to the method, the change of the fluorescence intensity canbe observed by naked eyes, and semi-quantitative visual analysis of the content of NO2<-> can be realized. The method is high in sensitivity, convenient to operate and applicable to quick detection of NO2<-> in food and environment.

Description

technical field [0001] The invention specifically relates to a carbon quantum dot for rapidly detecting nitrite content in food and the environment and an application method thereof, belonging to the fields of analytical chemistry and nanotechnology. Background technique [0002] Nitrate (NO 3 - ) and nitrite (NO 2 - ) widely exists in food and environment, and is the most common nitrogen-containing compound in nature. NO 3 - Widely used in agriculture as a nitrogen fertilizer for plant growth; NO 2 - It has antiseptic properties and is usually added to meat products as a food color enhancer to maintain a good appearance. In addition, NO 2 - It can prevent the formation of Clostridium botulinum and improve the safety of meat products. But it is worth noting that when NO 3 - In the presence of reductase, NO 3 - Reduced to NO in the digestive system and / or microorganisms 2 - . NO 2 - It can also be converted into N-nitrosamines in the acidic environment of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 郭隆华占园进邱彬林振宇
Owner FUZHOU UNIV
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