Electrospinning dressing loading growth factor small molecule inhibitor, preparation method and applications thereof

A technology of small molecule inhibitor and growth factor, applied in the field of medical biology, can solve the problems of cumbersome preparation process, complex composition, inability to achieve slow release, etc., and achieve the effect of good hydrophilicity and good thermal stability.

Inactive Publication Date: 2019-02-26
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Scholars at home and abroad have made some explorations in wound dressings for the treatment of hypertrophic scars, but there are the following problems: the composition is complex, and the influence

Method used

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  • Electrospinning dressing loading growth factor small molecule inhibitor, preparation method and applications thereof
  • Electrospinning dressing loading growth factor small molecule inhibitor, preparation method and applications thereof
  • Electrospinning dressing loading growth factor small molecule inhibitor, preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0070] Example 1

[0071] The device figure of the present invention design is as figure 1 shown. The spinning process is illustrated below by specific examples, and the steps are as follows:

[0072] (1) In a Erlenmeyer flask, 0.75 g of polycaprolactone (PCL, molecular weight 80000, Sigma), 0.25 g of gelatin (gelatin, type A powder, from pig skin, Sigma) and 5 ml of 5 μM l TGF-β1 inhibitor (TGF-β inhibitor, 6-[2-tert-butyl-5-(6-methyl-pyridin-2-yl)-1H-imidazol-4-yl]-quinoxalin, SB525334, Selleck Chemicals ), added 10 ml of hexafluoroisopropanol (1,1,1,2,2,2-Hexafluoro-2-propanol, HFIP, Sigma), and stirred for 2 hours at room temperature with a magnetic stirrer to make PCL, gelatin and TGF-β1 inhibitor were dissolved in HFIP and mixed uniformly to prepare a transparent spinning precursor solution.

[0073] (2) Inhale the configured electrospinning precursor solution into a syringe fixed on the propulsion pump, and the propulsion rate of the propulsion pump is 1.2 millili...

Example Embodiment

[0077] Example 2

[0078] Proceed as follows:

[0079] (1) In an Erlenmeyer flask, mix 1.2 grams of polylactic-co-glycolic acid (PLGA, 50 / 50, 12000, Sigma), 0.3 grams of collagen (type I, Sigma) and 5 milliliters of 5 μmol / L The TGF-β1 inhibitor was added into 10 ml of hexafluoroisopropanol, stirred at room temperature with a magnetic stirrer for 2 hours, so that PLGA and collagen were dissolved in HFIP and mixed evenly, and prepared into a transparent spinning precursor solution.

[0080] (2) The spinning process is the same as in Example 1.

[0081] (3) The performance characterization of the composite fiber membrane is the same as in Example 1, such as Figure 5 shown.

Example Embodiment

[0082] Example 3

[0083] Proceed as follows:

[0084] (1) In a Erlenmeyer flask, add 1.0 grams of PLGA, 0.5 grams of gelatin and 5 milliliters of 5 micromole / liter TGF-β1 inhibitor into 10 milliliters of hexafluoroisopropanol, and use a magnetic stirrer at room temperature Under stirring for 2 hours, PLGA and gelatin were dissolved in HFIP and mixed uniformly to prepare a transparent spinning precursor solution.

[0085] (2) The spinning process is the same as in Example 1.

[0086] (3) The performance characterization of the composite fiber membrane is the same as in Example 1, such as Image 6 shown.

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Abstract

The invention provides an electrospinning dressing loading a growth factor small molecule inhibitor, a preparation method and applications thereof. According to the present invention, the fiber membrane prepared by the electrospinning method has stable physical and chemical properties and high porosity, can isolate contaminants, and is soft and gas-permeable; the fiber membrane prepared by combining the macromolecule polymer and the natural polymer material has strong mechanical performance, wherein the natural polymer can enhance the cytocompatibility of the spinning fiber membrane, such thatthe cells easily grow on the fiber membrane so as to promote the wound healing; the TGF-beta1 inhibitor signaling pathway is clear and is targeted, and can effectively inhibit the scar formation; thefiber membrane has good wettability, can be perfectly matched with wounds with different shapes, and is prepared from the degradable material so as to reduce the secondary damage caused by dressing changing; and the preparation method is simple, the use is convenient, and the product has huge clinical application prospects.

Description

technical field [0001] The invention belongs to the field of medical biology, and in particular relates to an electrospinning dressing loaded with small molecule inhibitors of growth factors, and a preparation method and application thereof. Background technique [0002] The skin is the largest organ of the human body and has physiological functions such as barrier protection, temperature regulation, secretion and excretion, absorption and metabolism, and immunity. The wound healing process is divided into three separate but overlapping periods: (1) Inflammation period: occurs in 24-48 hours, its signs are local temperature increase, redness, swelling, the function of this stage is to remove dead bacteria and cells , to promote the healing process. (2) Proliferative phase: About 48 hours after trauma, this phase is characterized by the activity of fibroblasts, mainly manifested as re-epithelialization and granulation tissue formation. (3) Remodeling period: It begins about...

Claims

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Application Information

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IPC IPC(8): A61L15/42A61L15/44A61L15/62A61L15/26A61L15/32
CPCA61L15/26A61L15/32A61L15/42A61L15/425A61L15/44A61L15/62A61L2300/40A61L2300/604C08L67/04C08L89/00
Inventor 蒋兴宇王乐
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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