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Method for building fluorescent sensor based on competitive recognition and application

A fluorescent sensor and construction method technology, applied in the application field of insulin detection, can solve the problems of slow detection speed, low insulin recognition, complex insulin detection method, etc., and achieve the effect of simple method, broad market application prospect and high sensitivity

Active Publication Date: 2019-03-01
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problems that the existing insulin detection method is too complicated, the detection speed is slow, and the insulin recognition is low, and a method for constructing a fluorescent sensor based on competitive recognition is provided.

Method used

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  • Method for building fluorescent sensor based on competitive recognition and application
  • Method for building fluorescent sensor based on competitive recognition and application
  • Method for building fluorescent sensor based on competitive recognition and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: The construction method of this fluorescent sensor based on competitive recognition is as follows:

[0030] (1) Put 10g of hydroquinone bis(2-hydroxyethyl)ether, 12.5g of triphenylphosphine, and 150mL of anhydrous acetonitrile into the flask in sequence, cool in an ice-water bath, stir and mix well, then add 25g of carbon tetrabromide , stirred at room temperature for 5 hours, after the reaction was complete, cold water was added to the mixture to quench the reaction, and a white precipitate was obtained, which was collected by filtration, washed 4 times with methanol aqueous solution (volume ratio 3:2), recrystallized with methanol, and dried Compound 1 was obtained afterward; the nuclear magnetic resonance spectrum of compound 1 1 H NMR and 13 C NMR ( figure 1 );

[0031] (2) Add 5g of compound 1 and 0.92g of paraformaldehyde into the flask, use 300mL of chloroform as the solvent, then add 4.38g of boron trifluoride ether, and react at room temperatur...

Embodiment 2

[0035] Embodiment 2: The construction method of this fluorescent sensor based on competitive recognition is as follows:

[0036] (1) Put 15g of hydroquinone bis(2-hydroxyethyl)ether, 35g of triphenylphosphine, and 300mL of anhydrous acetonitrile into a round-bottomed flask in turn, cool in an ice-water bath, stir and mix well, then add 75g of tetrabromide Carbon, stirred at room temperature for 4 hours, after the reaction was complete, 250 mL of cold water was added to the mixture to quench the reaction, and a white precipitate was obtained, which was collected by filtration, washed 3 times with methanol aqueous solution (volume ratio 3:2), and reconstituted with methanol Crystallization, white crystals 15.6g after drying, compound 1 1 H NMR and 13 C NMR ( figure 1 );

[0037] (2) Add 4g of compound 1 and 1g of paraformaldehyde into the flask, 350mL of chloroform as the solvent, then add 5g of boron trifluoride ether, and react at room temperature for 3 hours under nitrogen...

Embodiment 3

[0040] Example 3: Experiments on the Binding Constants of Rhodamine B and Cationized Pillar [6] Arenes, Insulin and Cationized Pillars [6] Arenes

[0041] Add 2 μmol / L CP6 solution to 2mL 10μmol / L RhB solution in batches, adding 10μl each time, to obtain a set of fluorescence absorption curves, and draw the corresponding linear relationship according to the curves ( Figure 9 A) to calculate the binding constant Ka between RhB and CP6 1 ( Figure 9 B), the excitation wavelength of this process is 510 nm. In the same way, add 2 μmol / L insulin solution to 2 mL 10 μmol / L CP6 solution in batches, adding 10 μl each time, to obtain a set of fluorescence absorption curves ( Figure 10 A), also make the corresponding linear relationship according to the curve, and calculate the binding constant Ka between CP6 and Ins 2 ( Figure 10 B); the binding constant Ka between CP6 and Ins 2 Greater than the binding constant Ka between RhB and CP6 1 ; The different competitive relationship...

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Abstract

The invention discloses a method for building fluorescent sensor based on a competitive recognition and application. The method comprises the following steps: preparing a cation pillar [6] arene / reduced graphene oxide composite material CP6@rGO by a mild hydrothermal reaction; forming a receptor between the CP6 and rGO through pi-pi piling and electrostatic interaction; combining a fluorescent dyemolecule RhB with a receptor material for fluorescence resonance energy transfer to realize fluorescence quenching; when competitive analyte insulin is added, replacing the fluorescent dye molecule with analyte for recovering the fluorescent strength of the RhB; utilizing a fluorescent changing signal of insulin recovering the fluorescent strength of the RhB to perform quantitative determinationon the insulin. The defects of complexity, low detection speed and low insulin recognition property in an insulin detection method in the prior art are overcome. The method is simple, convenient, rapid, high in controllability and efficient, is suitable for industrial production, and has a wide market application prospect.

Description

technical field [0001] The invention belongs to the field of fluorescence sensors, and in particular relates to a construction method of a fluorescence sensor based on competitive recognition and its application in insulin detection. Background technique [0002] Insulin, an important protein hormone secreted by pancreatic beta cells, has a wide range of effects on metabolism and other bodily functions. It not only enables the cells in the liver, muscle and other adipose tissues to absorb glucose from the blood and convert it into glycogen as an energy source or into other nutrients stored in the cells, but also promotes the oxidative decomposition of glucose to release energy to maintain life activity. Insulin is a polypeptide composed of 51 amino acids, which is composed of a 21-residue A chain and a 30-residue B chain linked by two disulfide bonds. Insulin can be used as a predictor of insulinoma and traumatic diabetes. It has been reported that once the blood insulin ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N21/6486G01N2021/6432
Inventor 李灿鹏谭双吴石莲赵卉
Owner YUNNAN UNIV
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