Method for building fluorescent sensor based on competitive recognition and application

A fluorescent sensor and construction method technology, applied in the application field of insulin detection, can solve the problems of slow detection speed, low insulin recognition, complex insulin detection method, etc., and achieve the effect of simple method, broad market application prospect and high sensitivity
CN109406470AActive Publication Date: 2019-03-01YUNNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
YUNNAN UNIV
Publication Date
2019-03-01

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Abstract

The invention discloses a method for building fluorescent sensor based on a competitive recognition and application. The method comprises the following steps: preparing a cation pillar [6] arene / reduced graphene oxide composite material CP6@rGO by a mild hydrothermal reaction; forming a receptor between the CP6 and rGO through pi-pi piling and electrostatic interaction; combining a fluorescent dyemolecule RhB with a receptor material for fluorescence resonance energy transfer to realize fluorescence quenching; when competitive analyte insulin is added, replacing the fluorescent dye molecule with analyte for recovering the fluorescent strength of the RhB; utilizing a fluorescent changing signal of insulin recovering the fluorescent strength of the RhB to perform quantitative determinationon the insulin. The defects of complexity, low detection speed and low insulin recognition property in an insulin detection method in the prior art are overcome. The method is simple, convenient, rapid, high in controllability and efficient, is suitable for industrial production, and has a wide market application prospect.
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Description

technical field

[0001] The invention belongs to the field of fluorescence sensors, and in particular relates to a construction method of a fluorescence sensor based on competitive recognition and its application in insulin detection. Background technique

[0002] Insulin, an important protein hormone secreted by pancreatic beta cells, has a wide range of effects on metabolism and other bodily functions. It not only enables the cells in the liver, muscle and other adipose tissues to absorb glucose from the blood and convert it into glycogen as an energy source or into other nutrients stored in the cells, but also promotes the oxidative decomposition of glucose to release energy to maintain life activity. Insulin is a polypeptide composed of 51 amino acids, which is composed of a 21-residue A chain and a 30-residue B chain linked by two disulfide bonds. Insulin can be used as a predictor of insulinoma and traumatic diabetes. It has been reported that once the blood insulin ...

Claims

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