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Oil suspending agent of Isariajavanica conidiospore and application of oil suspending agent

A technology of conidia and oil suspension agent, which is applied to the conidia oil suspension agent of Isaria javanica, the field of fungal insecticides, can solve the problem of low germination rate of spores of entomogenous fungi, restricting the use of entomogenous fungi, and inconvenience. It is suitable for industrial production and other problems, and achieves the effects of wide source of raw materials, long storage period and simple preparation method.

Active Publication Date: 2019-03-19
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although the oil suspension has many advantages, it has also successfully overcome the defects that fungal spores are difficult to use in a dry environment and the insecticidal effect is affected by the humidity in the environment, but the oil suspension has the following problems: (1) preparation The cost of the oil is higher, and it needs to be diluted with oil or a special diluent for spraying after field application, which is not suitable for industrial production; (2) the germination rate of the entomogenous fungal spores in the preparation oil is low, and the storage stability is poor; restricts the use of entomogenic fungi

Method used

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  • Oil suspending agent of Isariajavanica conidiospore and application of oil suspending agent
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  • Oil suspending agent of Isariajavanica conidiospore and application of oil suspending agent

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: Preparation and quality inspection of pure powder of conidia of I. javanica

[0031] 1. Preparation method of pure powder of conidia of I. javanica

[0032] (1) First-level solid culture. Get conidia from I. javanica slant bacteria and inoculate them on sterilized PDA (potato 200g, glucose 20g, agar 20g, water 1000mL) plates, and cultivate them in a 25°C incubator for 2 About a week, scrape off the conidia and set aside.

[0033] (2) Secondary liquid culture, get the above-mentioned cultivated conidia, suspend in aseptic 0.02% (v / v) Tween-80 solution, and then inoculate in sterilized PD liquid culture medium (potato 200g, Glucose 20g, water 1000mL), at 25±1°C, 170r / min shaking culture for 48h as seed liquid, set aside.

[0034] (3) Three-stage rice fermentation spore production culture, soak the rice in clean water for 8 hours, then put it into a polypropylene plastic bag, 400g per bag, sterilize under high temperature and high pressure, inoculate each b...

Embodiment 2

[0046] Embodiment 2: Screening of the conidia oil suspending agent of I. javanica

[0047] Spore powder for testing: the pure powder of conidia prepared in Example 1.

[0048] Dispersants to be tested: sodium methylene bis-naphthalene sulfonate, abietic acid, sodium lauryl sarcosinate, sodium butylnaphthalene sulfonate, fatty alcohol polyvinyl ether, all purchased from Guangzhou Kehao Biotechnology Co., Ltd.

[0049]Thickeners for testing: xanthan gum and aluminum stearate were purchased from Guangzhou Kehao Biotechnology Co., Ltd.

[0050] Oil bases for testing: blended oil, peanut oil, soybean oil, corn oil.

[0051] 1. Preparation of oil suspension

[0052] Take a 1.5mL centrifuge tube, add the collected 5% pure conidia powder of I. javanica, then add 5% dispersant and 5% thickener, and finally add 85% oil base to make up to 1mL, and put it on the shaker Shake well on the instrument, so that the spore powder and the added additives are fully dispersed in the oil base. Th...

Embodiment 3

[0069] Embodiment 3: Indoor toxicity test and field drug efficacy test of Isychosporium javanica conidia oil suspension

[0070] 1. Insects to be tested:

[0071] Adults of Spodoptera litura were collected from the farm of South China Agricultural University in Guangzhou, and were reared with mulberry leaves to lay eggs. After hatching, the 3rd instar larvae with the same growth were selected for indoor virulence testing.

[0072] 2. Test preparation:

[0073] Treatment group: According to 28 kinds of formulas, prepare 1mL of 5% Isydiospora javanica conidia oil suspension, add 100mL of 0.02% Tween-80 aqueous solution, mix thoroughly with a vortex shaker, adjust to 1.0 with a hemocytometer ×10 7 conidia suspension per mL; control group: the concentration of pure spore powder was 1.0×10 7 pcs / mL suspension; clean water was used as the blank control.

[0074] 3. Processing method:

[0075] Take out the conidia oil suspension of I. javanica that has been placed at room temper...

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Abstract

The invention belongs to the technical field of biological control of biopesticides and pests and relates to an oil suspending agent of Isariajavanica conidiospore and an application of the oil suspending agent. The oil suspending agent of Isariajavanica conidiospore, disclosed by the invention, comprises the following components in percentage by mass: 3-10% of pure Isariajavanica conidiospore powder, 3-10% of dispersant, 3-10% of thickening agent and 80-90% of oil base. Isariajavanica belongs to Fungi, Dikarya, Ascomycota, Pezizomycotina, Sordariomycetes, Hypocreomycetidae, Hypocreales, Cordycipitaceae and Isaria in taxonomy. The oil suspending agent provided by the invention is used for preventing and controlling prodenia litura, and the pathogenicity of the oil suspending agent for three instar larvae of the prodenia litura reaches up to 80% or above.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides and pest biological control, and relates to a fungal insecticide, in particular to a conidia oil suspension of Isaria javanica and an application thereof. Background technique [0002] Isaria javanica is a common insect pathogenic fungus, which is widely distributed in nature. It is not safe for humans and animals, and will not cause environmental pollution. It invades from the epidermis of pests and causes insects to disease and die. It is a creature with "similar to contact action" Pesticides will not cause insect resistance, so this fungus has received more and more attention, and has the potential to develop into fungal insecticides. However, because fungal insecticides are living organisms, they are sensitive to environmental factors such as high temperature, drought, and ultraviolet rays, and their application in the field is restricted. Therefore, it is necessary to process th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N25/04A01P7/04
CPCA01N25/04A01N63/30
Inventor 胡琼波马竹炘蔡伟
Owner SOUTH CHINA AGRI UNIV
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