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Preparation method of sol-gel molecularly imprinted microspheres for detecting melamine

A technology of molecularly imprinted microspheres and sol-gel method, which is applied in the field of materials science, can solve the problems of insufficient selective recognition function, easy inactivation of protein antigens, cumbersome preparation process, etc.

Inactive Publication Date: 2019-03-22
TIANJIN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the selective recognition function of the above-mentioned biosensor is insufficient, and the protein antigen is easily inactivated in the environment of strong acid, strong alkali, high temperature and low temperature, and the preparation process is cumbersome.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Measure 30mL absolute ethanol, 50mL ultrapure water, 10mL NH 3 ·H 2 O was added to the flat-bottomed flask, and magnetically stirred evenly at room temperature; then, measure 5mL of TEOS and 20mL of absolute ethanol into a constant pressure separatory funnel, drop evenly into the flat-bottomed flask, and stir the mixture for 6h; then add 5mL APTES was magnetically stirred at room temperature for 12h. The obtained aminated SiO 2 The nanoparticles were centrifuged repeatedly with ethanol, washed and purified, and then dried in vacuum at 40°C. Weigh 0.1g SiO2 2 For nanoparticles, measure 30mL of ultrapure water into the conical flask, and sonicate for 10min; then, weigh 20mg of MEL, measure 160μL of APTES, add it into the conical flask and stir for 30min; then add 1.6mL of CTAB (0.2mol / L) , 0.2mL NaOH (2mol / L), stirred for 30min; then added 500μL TEOS, 200μL NH 3 ·H 2 O was stirred in the dark for 12h to obtain a polymer. The resulting imprinted polymer w...

Embodiment 2

[0015] Embodiment 2: Measure 30mL absolute ethanol, 50mL ultrapure water, 10mL NH 3 ·H 2 O was added to the flat-bottomed flask, and magnetically stirred evenly at room temperature; then, measure 5mL of TEOS and 20mL of absolute ethanol into a constant pressure separatory funnel, drop evenly into the flat-bottomed flask, and stir the mixture for 6h; then add 5mL APTES was magnetically stirred at room temperature for 12h. The obtained aminated SiO 2 The nanoparticles were centrifuged repeatedly with ethanol, washed and purified, and then dried in vacuum at 40°C. Weigh 0.1g SiO2 2 For nanoparticles, measure 30mL of ultrapure water into the conical flask, and sonicate for 10min; then, weigh 20mg of MEL, measure 160μL of APTES, add it into the conical flask and stir for 30min; then add 1.6mL of CTAB (0.2mol / L) , 0.2mL NaOH (2mol / L), stirred for 30min; then added 200μL TEOS, 200μL NH 3 ·H 2 O was stirred in the dark for 12h to obtain a polymer. The resulting imprinted polyme...

Embodiment 3

[0016] Embodiment 3: Measure 30mL absolute ethanol, 50mL ultrapure water, 10mL NH 3 ·H 2 O was added to the flat-bottomed flask, and magnetically stirred evenly at room temperature; then, measure 5mL of TEOS and 20mL of absolute ethanol into a constant pressure separatory funnel, drop evenly into the flat-bottomed flask, and stir the mixture for 6h; then add 5mL APTES was magnetically stirred at room temperature for 12h. The obtained aminated SiO 2 The nanoparticles were centrifuged repeatedly with ethanol, washed and purified, and then dried in vacuum at 40°C. Weigh 0.1g SiO2 2 For nanoparticles, measure 30mL of ultrapure water into the conical flask, and sonicate for 10min; then, weigh 20mg of MEL, measure 160μL of APTES, add it into the conical flask and stir for 30min; then add 1.6mL of CTAB (0.2mol / L) , 0.2mL NaOH (0.2mol / L), stirred for 30min; then added 500μL TEOS, 200μL NH 3 ·H 2 O was stirred in the dark for 12h to obtain a polymer. The resulting imprinted poly...

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Abstract

The invention discloses a preparation method of sol-gel molecularly imprinted microspheres for detecting melamine. The preparation method adopts the following technology and the technology comprises the following steps: (1) preparing aminated SiO2 nanoparticles; (2) preparing a melamine molecularly imprinted material MEL-MIPs / SiO2. According to the preparation method, a sol-gel method is adopted,3-aminopropyltriethoxysilane is used as a functional monomer, tetraethyl orthosilicate is used as a crosslinking agent, hexadecyl trimethyl ammonium bromide is used as a pore-forming agent and a sodium hydroxide solution is used as an initiator to prepare imprinted microspheres MEL-MIPs / SiO2 which have a specific identification capability and are used for carrying out sensitivity detection.

Description

technical field [0001] The invention relates to the field of material science, in particular to a method for preparing sol-gel molecularly imprinted microspheres capable of detecting melamine, in particular to a method for preparing aminated silicon dioxide nanoparticles, and then using a sol-gel method to prepare melamine-imprinted polymers new method. Background technique [0002] Melamine is a triazine-type nitrogen-containing heterocyclic organic chemical intermediate product with a uniform molecular structure. It has a wide range of uses. The most important use is that it can be used as an important raw material for the production of industrial products melamine-formaldehyde resin. In addition, it is also very useful in the production of plastics, coatings, adhesives, flame retardants and food packaging materials. At the same time, melamine is also a metabolite of cyromazine, a commonly used insecticide for plants and animals such as goats, chickens, and mice. , Some ag...

Claims

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Application Information

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IPC IPC(8): C08G77/26C08J9/28B01J13/04G01N21/33G01N21/3563
CPCB01J13/046C08G77/26C08J9/28C08J2383/08G01N21/33G01N21/3563G01N2021/3572
Inventor 宋丹丹王兵李艳庭
Owner TIANJIN POLYTECHNIC UNIV
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