Preparation method of sol-gel molecularly imprinted microspheres for detecting melamine
A technology of molecularly imprinted microspheres and sol-gel method, which is applied in the field of materials science, can solve the problems of insufficient selective recognition function, easy inactivation of protein antigens, cumbersome preparation process, etc.
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Embodiment 1
[0014] Example 1: Measure 30mL absolute ethanol, 50mL ultrapure water, 10mL NH 3 ·H 2 O was added to the flat-bottomed flask, and magnetically stirred evenly at room temperature; then, measure 5mL of TEOS and 20mL of absolute ethanol into a constant pressure separatory funnel, drop evenly into the flat-bottomed flask, and stir the mixture for 6h; then add 5mL APTES was magnetically stirred at room temperature for 12h. The obtained aminated SiO 2 The nanoparticles were centrifuged repeatedly with ethanol, washed and purified, and then dried in vacuum at 40°C. Weigh 0.1g SiO2 2 For nanoparticles, measure 30mL of ultrapure water into the conical flask, and sonicate for 10min; then, weigh 20mg of MEL, measure 160μL of APTES, add it into the conical flask and stir for 30min; then add 1.6mL of CTAB (0.2mol / L) , 0.2mL NaOH (2mol / L), stirred for 30min; then added 500μL TEOS, 200μL NH 3 ·H 2 O was stirred in the dark for 12h to obtain a polymer. The resulting imprinted polymer w...
Embodiment 2
[0015] Embodiment 2: Measure 30mL absolute ethanol, 50mL ultrapure water, 10mL NH 3 ·H 2 O was added to the flat-bottomed flask, and magnetically stirred evenly at room temperature; then, measure 5mL of TEOS and 20mL of absolute ethanol into a constant pressure separatory funnel, drop evenly into the flat-bottomed flask, and stir the mixture for 6h; then add 5mL APTES was magnetically stirred at room temperature for 12h. The obtained aminated SiO 2 The nanoparticles were centrifuged repeatedly with ethanol, washed and purified, and then dried in vacuum at 40°C. Weigh 0.1g SiO2 2 For nanoparticles, measure 30mL of ultrapure water into the conical flask, and sonicate for 10min; then, weigh 20mg of MEL, measure 160μL of APTES, add it into the conical flask and stir for 30min; then add 1.6mL of CTAB (0.2mol / L) , 0.2mL NaOH (2mol / L), stirred for 30min; then added 200μL TEOS, 200μL NH 3 ·H 2 O was stirred in the dark for 12h to obtain a polymer. The resulting imprinted polyme...
Embodiment 3
[0016] Embodiment 3: Measure 30mL absolute ethanol, 50mL ultrapure water, 10mL NH 3 ·H 2 O was added to the flat-bottomed flask, and magnetically stirred evenly at room temperature; then, measure 5mL of TEOS and 20mL of absolute ethanol into a constant pressure separatory funnel, drop evenly into the flat-bottomed flask, and stir the mixture for 6h; then add 5mL APTES was magnetically stirred at room temperature for 12h. The obtained aminated SiO 2 The nanoparticles were centrifuged repeatedly with ethanol, washed and purified, and then dried in vacuum at 40°C. Weigh 0.1g SiO2 2 For nanoparticles, measure 30mL of ultrapure water into the conical flask, and sonicate for 10min; then, weigh 20mg of MEL, measure 160μL of APTES, add it into the conical flask and stir for 30min; then add 1.6mL of CTAB (0.2mol / L) , 0.2mL NaOH (0.2mol / L), stirred for 30min; then added 500μL TEOS, 200μL NH 3 ·H 2 O was stirred in the dark for 12h to obtain a polymer. The resulting imprinted poly...
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