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New application of asiaticoside in the preparation of medicines for treating skin cancer

A technology of asiaticoside and skin cancer, applied in the field of medicine, can solve the problems of no asiaticoside and poor treatment effect

Active Publication Date: 2021-03-12
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these drugs have poor therapeutic effects, and local reactions such as erythema, burning, ulceration, and crusting are obvious
There is no report on asiaticoside in the treatment of skin cancer

Method used

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  • New application of asiaticoside in the preparation of medicines for treating skin cancer
  • New application of asiaticoside in the preparation of medicines for treating skin cancer
  • New application of asiaticoside in the preparation of medicines for treating skin cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The preparation of embodiment 1 asiaticoside ointment

[0024] This embodiment provides a kind of cream for external use of 4% (W / W) asiaticoside, made from the following raw material components:

[0025] 400 parts by weight of asiaticoside, 1000 parts by weight of stearic acid, 200 parts by weight of glyceryl stearate, 200 parts by weight of beeswax, 600 parts by weight of palm oil, 600 parts by weight of butanediol, 200 parts by weight of ethanol, and 4800 parts by weight of water Parts, 3 parts by weight of carbomer, 2 parts by weight of triethanolamine.

[0026] According to above-mentioned raw material component, prepare Chinese medicine external cream of the present invention:

[0027] (1) Weigh stearic acid, glyceryl stearate, beeswax, and palm oil in proportion, heat in a water bath to melt, filter, and store at a constant temperature of 70-80°C;

[0028] (2) Weigh butanediol, ethanol, water, carbomer, and triethanolamine in proportion and add them to the abov...

Embodiment 2

[0030] Example 2 The inhibitory effect of asiaticoside on mouse skin orthotopic tumors

[0031] 1. Experimental animals

[0032] SPF grade ICR mice, male, 6 weeks old, weighing 16-18 g, were purchased from Qinglongshan Experimental Animal Breeding Center. Raise in single cages, provide sufficient feed and drinking water, maintain a circadian rhythm of 12 hours of light and 12 hours of darkness, temperature 25±2°C, humidity 50%-70%, and the experimental process follows animal ethics.

[0033] 2. Main reagents

[0034] 7,12-Dimethylbenzanthracene (DMBA): Sigma, USA.

[0035] Phorbol ester (TPA): Sigma, USA.

[0036] Acetone: Sinopharm Chemical Reagent Co., Ltd.

[0037] Normal saline: Anhui Double Crane Pharmaceutical Co., Ltd.

[0038] Chloral hydrate: Shanghai Lingfeng Chemical Reagent Co., Ltd.

[0039] Capecitabine, oleanolic acid, phenformin, tamoxifen, docetaxel, oxaliplatin: Dalian Meilun Biotechnology Co., Ltd.

[0040] Prepared with reference to the method of Exa...

Embodiment 3

[0068] Example 3 The inhibitory effect of asiaticoside on colon cancer in mice

[0069] 1. Experimental animals: inbred NOD / SCID mice, female, 6 weeks old, weighing 16-18g, purchased from Beijing Weitong Lihua. Raise in single cages, provide sufficient feed and drinking water, maintain a circadian rhythm of 12 hours of light and 12 hours of darkness, temperature 25±2°C, humidity 50%-70%, and the experimental process follows animal ethics.

[0070] 2. Experimental process

[0071] (1) After the human colon cancer cell line HT29 cells were resuscitated and passaged, when the cells reached a stable growth state, they entered the next step of the experiment.

[0072] (2) Preparation of cell inoculum

[0073] Aspirate the cell supernatant, transfer the cells to a centrifuge tube, and centrifuge at 1000 rpm for 5 minutes; remove the supernatant, resuspend the cell mass with PBS, count, and adjust the cell density to 1×10 7 cells / mL, mix the cells evenly, take 100 μL of the cell s...

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Abstract

The invention discloses for the first time the application of asiaticoside in the preparation of medicaments for treating skin cancer. The results of the DMBA / TPA two-step chemically induced mouse skin cancer model show that the therapeutic effect of asiaticoside on skin cancer is significantly better than that of the drugs used to treat scars, other AMPK agonists, clinical drugs for other cancers, and similar structures. medicine. The present invention provides a new drug choice for clinical treatment of skin cancer.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the new application of asiaticoside in the preparation of medicines for treating skin cancer. Background technique [0002] Skin cancer is a malignant tumor that occurs in the skin. Clinically, it is mainly divided into malignant melanoma (Malignant melanoma, MM) and non-melanoma skin cancer (Non-melanoma skin cancer, NMSC), the latter mainly including basal cell carcinoma (Basal cell carcinoma). carcinoma, BCC) and squamous cell carcinoma (Squamous cell carcinoma, SCC). The first choice of treatment for skin cancer in modern medicine is surgery. Early, smaller, well-differentiated skin cancers can be treated with physical therapy, including cryotherapy, radiation, and laser therapy. The cure rate of radiotherapy for clinical early skin cancer, especially basal cell carcinoma, can reach 95%. It is suitable for patients with surgical contraindications or difficult surgical rese...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7024A61P35/00
CPCA61K31/7024A61P35/00
Inventor 吴亮李成婧蔡溪云弋方睿宁邓定煜牟畅崔雪婷葛峰张莹莹董浩蔡磊郝园园吴舒婷童玥
Owner CHINA PHARM UNIV
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