A kind of detection method of sodium nitrophenolate residue in fruits and vegetables

A technology of sodium nitrophenolate and a detection method, which is applied in the field of detection of sodium nitrophenolate residues in fruits and vegetables, can solve the problem that the maximum residue limit value of sodium nitrophenolate is not formally specified, the detection method is blank, and the dietary intake of sodium nitrophenolate is not available. The problem of incomplete input and risk data, etc., achieves the effect of simple and fast operation

Active Publication Date: 2021-01-22
SHANDONG YANTAI AGRI SCI & TECH INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the blank of the standard detection method for the residual amount of sodium nitrophenolate in the existing agricultural field, the dietary intake of sodium nitrophenolate and the risk data of residents are incomplete, and the maximum residual limit of sodium nitrophenolate on agricultural products Insufficiency that the value has no formal regulations, providing a fast, simple and accurate method for the detection of sodium nitrophenolate residues in fruits and vegetables

Method used

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  • A kind of detection method of sodium nitrophenolate residue in fruits and vegetables
  • A kind of detection method of sodium nitrophenolate residue in fruits and vegetables
  • A kind of detection method of sodium nitrophenolate residue in fruits and vegetables

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Experimental program
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Effect test

Embodiment 1

[0030] 1. Main equipment and materials

[0031] Shimadzu LC / MS 8040; German IKA T25 high-speed homogenizer; Guohua SK-1 fast mixer; HX-PB965 Oakes broken wall nutrition cooking machine; Sartorius BL610 electronic analytical balance; Merck Milli-Q ultra-pure water system.

[0032] Methanol, acetone, ammonium acetate and formic acid were all chromatographically pure.

[0033] 2. Measurement method

[0034] (1), standard curve drawing

[0035] Take by weighing three kinds of active ingredient standard items of compound sodium nitrophenolate (sodium o-nitrophenolate, sodium p-nitrophenolate, sodium 5-nitro-o-methoxyphenolate) and dilute with acetonitrile to obtain 10 mg / L complex sodium nitrophenolate solution, Dilute the 10 mg / L sodium nitrophenolate solution with acetonitrile to obtain a series of standard solutions of sodium nitrophenolate from 0.001 mg / L to 1.0 mg / L, and perform liquid chromatography tandem mass spectrometry analysis and detection. The concentration points...

Embodiment 2

[0054] 1. Main equipment and materials

[0055] Shimadzu LC / MS 8040; German IKA T25 high-speed homogenizer; Guohua SK-1 fast mixer; HX-PB965 Oakes broken wall nutrition cooking machine; Sartorius BL610 electronic analytical balance; Merck Milli-Q ultra-pure water system.

[0056] Methanol, acetone, ammonium formate and formic acid were all chromatographically pure.

[0057] 2. Measurement method

[0058] (1) standard curve drawing (same as embodiment 1)

[0059] (2) Sample extraction

[0060] Weigh 20.0g of the grape sample homogenized by the breaker, add 20ml of acetonitrile, vortex and homogenize for 1min, then filter with filter paper, collect the filtrate into a 100mL stoppered measuring cylinder containing 6g of sodium chloride, cover with a stopper, Vigorously shake for 1 min, and stand at room temperature for 30 min to separate the acetonitrile phase and the water phase;

[0061] (3), purification

[0062] Draw 10.0mL of acetonitrile solution from a stoppered meas...

Embodiment 3

[0076] 1. Main equipment and materials

[0077] Shimadzu LC / MS 8040; German IKA T25 high-speed homogenizer; Guohua SK-1 fast mixer; HX-PB965 Oakes broken wall nutrition cooking machine; Sartorius BL610 electronic analytical balance; Merck Milli-Q ultra-pure water system.

[0078] Methanol, acetone, ammonium carbonate and formic acid were all chromatographically pure.

[0079] 2. Measurement method

[0080] (1) standard curve drawing (same as embodiment 1)

[0081] (2) Sample extraction

[0082] Weigh 25.0 g of blueberry samples homogenized by a wall breaker, add 25 ml of acetonitrile, vortex and homogenize for 1 min, then filter with filter paper, collect the filtrate into a 100 mL stoppered measuring cylinder containing 5 g of sodium chloride, and cover with a stopper. Vigorously shake for 1 min, and stand at room temperature for 30 min to separate the acetonitrile phase and the water phase;

[0083] (3), purification

[0084] Draw 10.0mL of acetonitrile solution from a...

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Abstract

The invention relates to a method for detecting residue of compound sodium nitrophenolate in fruits and vegetables. The method comprises the following specific steps: (1) drawing a standard curve; (2)extracting a sample; (3) purifying; (4) measuring the recovery rate; and (5) measuring the content of the compound sodium nitrophenolate in the sample. According to the method for detecting residue of compound sodium nitrophenolate in fruits and vegetables, acetonitrile is used for extracting, and an extracting solution is purified by an amino solid phase extraction column and determined on a machine. The detection method is easy and convenient to operate. A multi-reaction monitoring mode in which a liquid-phase spectrum is in series connection with a mass spectrum is adopted, so that relatively high selectivity and sensitivity are achieved; impurity interference can be lowered effectively; higher accuracy and precision can be achieved; and technical support is provided for the further improvement and supplementation of the dietary intake of compound sodium nitrophenolate by residents and risk data as well as formulation of maximum residue limit.

Description

technical field [0001] The invention relates to the technical field of trace analysis, in particular to a method for detecting residual sodium nitrophenolate in fruits and vegetables. Background technique [0002] Sodium nitrophenolate is a plant growth regulator, which has a significant yield-increasing effect and is widely used in agriculture. At present, there is no standard detection method for sodium nitrophenolate residues in China, and the national standard GB 2763-2016 food safety national standard only stipulates the temporary limit of sodium nitrophenolate on five crops. In recent years, the national agricultural product quality and safety risk assessment shows that sodium nitrophenolate is frequently used on fruits and vegetables and is harmful to the eyes. It has become a high-sensitivity risk factor affecting the quality and safety of fruits and vegetables, and will be included in the key monitoring and evaluation objects. At present, Zhang Shuwen et al. have r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884
Inventor 兰丰刘传德周先学李晓亮王志新鹿泽启姚杰姜蔚柳璇
Owner SHANDONG YANTAI AGRI SCI & TECH INST
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