Polypeptide derived from moon jellyfish, and preparation process and application thereof

Abstract

The invention discloses a polypeptide derived from moon jellyfish, and a preparation process and application thereof. The polypeptide is prepared according to the following steps: (1) taking fresh orfrozen moon jellyfish, washing the moon jellyfish with sea water or deionized water, putting the moon jellyfish into a reaction kettle, and adding a chelating agent and a metal salt; (2) at 1-10 DEG C, starting stirring paddles to pulverize the moon jellyfish until no visible jelly-like particles in the mixture, and homogenizing the mixture; (3) filtering the mixture, retaining residues, and washing the residues with ionized water; (4) putting the washed residues into an enzyme reaction vessel, adding deionized water and protease, conducting a reaction at 35-60 DEG C for 2-10 h, conducting heating to 90-100 DEG C, keeping the temperature for 10-20 min, and conducting inactivation; (5) cooling the reaction products to 35-60 DEG C, adding an appropriate amount of a chelating agent enzyme, conducting reaction for 2-10 h, then conducting heating to 90-100 DEG C, keeping the temperature for 10-20 min, and conducting inactivation; and (6) conducting cooling to room temperature, adding a precipitant to the mixture, conducting stirring for 10-30 min, conducting filtering, and collecting the filtrate and conducting drying to obtain a moon jellyfish polypeptide. The moon jellyfish polypeptide can be widely used in the fields of cosmetics, health care products, and pharmaceuticals.

Description

technical field [0001] The invention relates to a moon jellyfish-derived polypeptide, a preparation method and application thereof, and belongs to the technical field of marine biology. Background technique [0002] Jellyfish appeared 650 million years ago. After five mass extinctions, they are still active today, which shows that their ability to resist harsh environments and self-healing functions are very powerful. In addition, the excessive reproduction of jellyfish has caused serious harm to marine fisheries, marine ecology, and industries around the ocean, so its development and utilization is particularly important. The water content of jellyfish is generally more than 96%. Among the rest of the non-water components, the content is more protein. The current applied research hotspots are all concentrated on jellyfish (such as jellyfish, sand stinger, rosy jellyfish, peach blossom jellyfish, fire jellyfish, cassia queen jellyfish, etc.) jellyfish) protein and peptide e...

AI Technical Summary

Problems solved by technology

However, problems such as high water content, easy autolysis, difficulty in preservation, and difficulty in desalting and dealuminizing after pickling have restricted the deep utilization of jellyfish and caused a lot of waste of resources.