Molecular marker of wheat recessive nuclear male sterility mutant gene ms1 and application of molecular marker

Abstract

The invention provides a molecular marker of wheat recessive nuclear male sterility mutant gene ms1 and an application of the molecular marker, and discloses a detection method of the molecular markerof the wheat recessive nuclear male sterility mutant gene ms1 and the application. Molecular markers of the four mutants ms1d. 1, ms1h, ms1m and ms1p are designed, and are all functional markers of the MS1 gene. The markers can specifically detect the wheat mutant ms1, the wild Ms1 gene and the mutant ms1 gene, and homozygous and heterozygous genotypes can be differentiated. The allelotype of thenuclear male sterility mutant gene ms1 or the fertile gene ms1 can be detected and marked accurately by the designed functional markers, and the molecular marker can be used for identification of wheat nuclear male sterile plant lines and screening of target single plants, and has important application value in transformation of the wheat male sterile line and sterile line hybrid seed productionand molecular marker-assisted breeding.

Description

technical field [0001] The invention belongs to the field of crop molecular breeding. In particular, it relates to a primer for molecular marker detection of wheat recessive nuclear male sterility gene ms1 and an application thereof. Background technique [0002] Wheat is an important food crop, and about 30% of the world's population uses wheat as the main food. China has a large population and is a big wheat producer and consumer, so wheat plays a pivotal role in grain production. The utilization of heterosis is an important way to increase the yield of wheat. In the world, large-scale breeding and planting of hybrid rice and hybrid corn have played an extremely important role in increasing grain yield. However, hybrid wheat has accounted for less than 0.2% of the total wheat planted area worldwide from the past few decades until now. This is because wheat lacks excellent stable male sterile lines to configure and produce wheat hybrids. Therefore, breeding excellent whe...

AI Technical Summary

Problems solved by technology

Therefore, starting from the discovery of wheat recessive and sterile lines, breeders used conventional breeding techniques to introduce the 4E chromosome containing the blue endosperm gene and fertility restorer gene in the wheat additional line into the wheat recessive genic male sterile line. Male sterile and maintainer lines of wheat were selected and bred, but the blue endosperm gene and the fertility restorer gene were incompletely linked, resulting in a decrease in the purity of the selected sterile line (Wang Pengke, Yang Zhiquan, Huang Shousong

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