A kind of hydrogel dressing with high oxygen transmission rate and preparation method thereof
A technology of oxygen transmission rate and hydrogel, which is applied in the field of high oxygen transmission rate hydrogel dressing and its preparation, and can solve the problems of low oxygen transmission rate, low light transmittance, poor mechanical properties, etc.
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Embodiment 1
[0030] A kind of preparation preparation method of polysiloxane macromonomer crosslinking agent, it comprises the following steps:
[0031] Add 7.5g of KF-6001 into a 100mL branch bottle, insert a mechanical stirrer, coat the cock with vacuum ester, connect the branch to a vacuum line, and dry in a 55°C water bath at 300rmp for 2 hours. Balance the air pressure to normal pressure for 30±5min, weigh 1.165g IEM by differential method, inject it into the reactor with a syringe, use a 100μL micro-sampler to absorb 100μL of dibutyltin dilaurate catalyst and drop it into the reactor, and stir at room temperature for 16h . Dissolve the product in the reaction bottle with 35 mL of n-propanol, transfer it to the product bottle, suction filter with a 5 μm filter membrane, and remove the solvent by rotary evaporation to obtain a polysiloxane macromer crosslinking agent.
Embodiment 2
[0033] A preparation method of a high oxygen permeability hydrogel dressing, which comprises the following steps:
[0034] Measure the polysiloxane macromonomer cross-linking agent in the embodiment 1 of 37.5g, the hydroxyethyl methacrylate (HEMA) of 17.8g, the methacryloxypropyl group tri(trimethylsilyl) of 20.6g Oxyalkyl) silane (Tris), 1.0 g of Darocur 1173 was dissolved in 23.1 g of n-propanol by mass percentage, magnetically stirred for 20 minutes, and ultrasonically defoamed for 10 minutes to obtain a polymerization solution for use. Use a pipette gun to draw 5mL of the polymerization solution and drop it into a polytetrafluoroethylene mold with a 5*5*0.2cm inner groove. After the drop is full, cover the mold with a 6*6*0.2cm glass plate, and place the whole under ultraviolet light. Under light, polymerize for 40min. Open the glass plate, take off the polymerized solid gel from the mold, wash it with ethanol for 15 minutes, and place it in physiological saline to obtain...
Embodiment 3
[0036] Material in embodiment 2 is carried out biocompatibility evaluation, cell used is mouse embryonic fibroblast (3T3), prepares cell suspension, inoculates in the 24-well plate that is placed dressing material in embodiment 2; Control group is Cells were seeded in 96-well plates without the dressing material in Example 2. 10000 cells per well. Place them in an incubator with 5% carbon dioxide at 37° C., replace with fresh 10% fetal bovine serum medium every 24 hours, and conduct a CCK-8 cell proliferation test. Detect the OD value at 450nm wavelength, the higher the OD value, the more the number of cells.
[0037] The polysiloxane macromer crosslinking agent synthesized by the present invention and the hydrogel dressing with high oxygen transmission rate have been characterized in detail, and the specific characterization methods are as follows:
[0038] Infrared Spectroscopy Analysis
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