Slow-release microorganism microbial inoculum for government of black and odorous water body and preparation method of slow-release microorganism microbial inoculum
A technology of microbial inoculum and microbial inoculum, applied in the field of slow-release microbial inoculum and its preparation, can solve the problems of long purification cycle, high treatment cost, poor treatment effect, etc. The effect of various types of taste and microorganisms
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Embodiment 1
[0047] A method for preparing a slow-release microbial bacterial agent for black and odorous water treatment, which specifically includes the following steps:
[0048] (1) Saccharomyces, Enterobacter, Pseudomonas oleovorans, Bacillus coagulans, Bacillus subtilis B38, Nitrospirilla ( Nitrosospira), Nitrosolobus, Nitrosovibrio, Thauera, Flexibacter, Comamonas, Sphingosine Bacillus (Sphingobacterium sp) was inoculated into the liquid culture medium in the Erlenmeyer flask respectively, and cultured at 20-32°C for 48-72 hours to obtain a seed culture solution, which was set aside;
[0049] (2) Inoculate the seed culture solution of each genus into the liquid fermentation medium of each genus according to the inoculation amount of 10%, and cultivate it at 20-35°C for 60-96h to the logarithmic growth phase to obtain the bacterial agent culture solution , were centrifuged, concentrated, and mixed according to the ratio of 1, 1, 1, 2, 3, 3, 1, 1, 1, 5, 2, 5;
[0050] (3) The concent...
Embodiment 2
[0055] A method for preparing a slow-release microbial bacterial agent for black and odorous water treatment, which specifically includes the following steps:
[0056] (1) Saccharomyces, Enterobacter, Pseudomonas oleovorans, Bacillus coagulans, Bacillus subtilis B38, Nitrospirilla ( Nitrosospira), Nitrosolobus, Nitrosovibrio, Thauera, Flexibacter, Comamonas, Sphingosine Bacillus (Sphingobacterium sp) was inoculated into the liquid culture medium in the Erlenmeyer flask respectively, and cultured at 20-32°C for 48-72 hours to obtain a seed culture solution, which was set aside;
[0057] (2) Inoculate the seed culture solution of each genus into the liquid fermentation medium of each genus according to the inoculation amount of 10%, and cultivate it at 20-35°C for 60-96h to the logarithmic growth phase to obtain the bacterial agent culture solution , were centrifuged, concentrated, and mixed according to the ratio of 2, 2, 1.5, 3, 4, 4, 2, 2, 2, 6, 3, 6;
[0058] (3) The conce...
Embodiment 3
[0063] A method for preparing a slow-release microbial bacterial agent for black and odorous water treatment, which specifically includes the following steps:
[0064] (1) Saccharomyces, Enterobacter, Pseudomonas oleovorans, Bacillus coagulans, Bacillus subtilis B38, Nitrospirilla ( Nitrosospira), Nitrosolobus, Nitrosovibrio, Thauera, Flexibacter, Comamonas, Sphingosine Bacillus (Sphingobacterium sp) was inoculated into the liquid culture medium in the Erlenmeyer flask respectively, and cultured at 20-32°C for 48-72 hours to obtain a seed culture solution, which was set aside;
[0065] (2) Inoculate the seed culture solution of each genus into the liquid fermentation medium of each genus according to the inoculation amount of 10%, and cultivate it at 20-35°C for 60-96h to the logarithmic growth phase to obtain the bacterial agent culture solution , were centrifuged, concentrated, and mixed according to the ratio of 5, 3, 1, 1, 2, 4, 1, 1, 1, 1, 4, 2;
[0066] (3) The concent...
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