SNP marker developing and application of rice bacterial blight resistance gene xa5

Abstract

The invention provides an SNP molecular marker K_050527 which is tightly linked to a rice bacterial blight resistance gene xa5. It is detected by the SNP marker that the basic group at the 437500site of the 5 chromosome of rice is C or G, and the primer combination of the SNP marker developed based on a KASP technology is Primer X, Primer Y and Primer C. According to the SNP marker, the KASP technology is utilized to perform rapid genotyping on the SNP marker which is tightly linked to the rice bacterial blight resistance gene xa5, the SNP marker can be applied to commercial molecularseedling breeding with high, medium or low through-put, the selectivity efficiency of a developed SNP marker phenotype reaches up to 100%, and therefore the rice bacterial blight resistance gene xa5can be rapidly and accurately detected in different germ plasm resources of indica rice, japonica rice and the like; the complicated procedures of enzyme digestion, electrophoresis, sequencing and thelike are not needed in the detecting process, aerosol contamination is reduced, the use of toxic substances of ethidium bromide EB (ethidium bromide) and the like is reduced, outlook molecular markerauxiliary selection at the early stage of seedling breeding can be conducted to reduce the field planting scale of seedling breeding groups and the seedling breeding cost, and the seedling breeding process is accelerated.

Description

technical field [0001] The invention relates to the fields of molecular markers and crop breeding, in particular to the development and application of a SNP marker of rice bacterial blight resistance gene xa5. Background technique [0002] Rice bacterial blight is one of the three major diseases of rice, caused by Xanthomonas (Xoo, Xanthomonas oryzaepv.Oryzae), and is the most important rice bacterial disease in the world. Reduce production by 20%-50%, and in severe cases, it may cause no harvest. However, there is still a lack of chemical pesticides to effectively control rice bacterial blight in current production, so cultivating and planting disease-resistant varieties is the most economical and effective measure to control bacterial blight. In recent decades, with the development of molecular genetics, molecular marker-assisted selection has played a pivotal role in rice disease resistance breeding. The use of efficient molecular marker-assisted selection of single plan...

AI Technical Summary

Problems solved by technology

At present, most of the markers used to detect the xa5 gene are RFLP and CAPS markers, which have a certain physical distance from xa5, and the detection results are likely to cause misjudgment. RFLP and CAPS markers can only be used for polymorphism analysis between specific parental combinations. Experimental operations It is cumbersome and the detection throughput is small, so it is not suitable for large-scale identification of germplasm resources or screening of resistant resources

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