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Mechanical perfusion system for alleviating postoperative infection of organ transplantation

An organ perfusion and mechanical technology, applied in the field of medical device design and manufacturing, can solve the problems of poor antibiotic treatment effect, strong resistance of infected bacteria, low temperature stability, etc., and achieve good market prospects and social public welfare value. The problem of drug resistance, the effect of good temperature environment

Active Publication Date: 2019-04-19
嘉兴莱普晟医疗科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the problems in the prior art that the organ transplant surgery is prone to postoperative infection, and the infection bacteria have strong drug resistance, the treatment effect of antibiotics is not good, and the internal temperature stability is not high during the working process of the mechanical perfusion system. Problem, the inventors of the present invention can solve the above problems well by using the coating prepared by graphene oxide and antimicrobial peptides to prepare the mechanical perfusion system. In view of this, the present invention provides a mechanical perfusion system, which is characterized in that , the mechanical perfusion system includes a coating made of graphene oxide and antimicrobial peptides, specifically, the perfusion system includes an organ perfusate storage and an organ storage device, and the organ perfusate storage and the organ storage device contain graphene oxide and Antimicrobial peptides, preferably, graphene oxide and antimicrobial peptides are prepared as coatings coated on the inner walls of organ perfusate storage and organ storage devices

Method used

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  • Mechanical perfusion system for alleviating postoperative infection of organ transplantation
  • Mechanical perfusion system for alleviating postoperative infection of organ transplantation
  • Mechanical perfusion system for alleviating postoperative infection of organ transplantation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The preparation of embodiment 1 composite antimicrobial peptide

[0044] The mutated compound antimicrobial peptide was synthesized by peptide synthesis method, and the synthesized peptide was named CT-1. The peptide synthesis adopts the F-moc automatic solid-phase synthesis method, and the specific method is as follows:

[0045] 1.1 Synthesis of peptides

[0046] Protected polypeptides were assembled on resin using a model of the 433A automated synthesizer (ABI, Foster City, CA). The peptide resin was incubated in suspension for 2.5 hours at room temperature to deprotect the group. The suspension system is composed of 10 ml of TFA, 0.75 g of phenol, 0.25 ml of 1,2-ethanedithiol, 0.5 ml of sulfide anisole and 0.5 ml of water. The resin was separated from the peptide deprotected mixture by filtration. The crude polypeptide was precipitated in 150ml pre-cooled ether solution, and purified by chromatography on a Sephadex G-25 column with 10% glacial acetic acid as elue...

Embodiment 2

[0052] Example 2 Preparation of thermally conductive material covered with graphene oxide-loaded antimicrobial peptide coating

[0053] In this implementation, the reduced graphene oxide-loaded antimicrobial peptide coating is prepared first, and then the coating is applied to the surface of the heat-conducting material by self-assembly. The heat-conducting material is aluminum alloy, and the graphene oxide-loaded antimicrobial peptide coating is obtained. The aluminum alloy material, concrete preparation method is as follows:

[0054] 2.1 Surface modification of thermally conductive materials

[0055] First immerse the cleaned and dried aluminum alloy in 2mg / ml dopamine hydrochloride solution, adjust the pH value to -8.5 with NaOH, and use it after reacting overnight at room temperature. Repeat three times to modify the surface of the aluminum alloy with polydopamine, then immerse the aluminum alloy material with polydopamine on the surface into a 5 mg / ml polylysine solution...

Embodiment 3

[0064] Comparison of the antibacterial effect of the coating prepared by the composite antimicrobial peptide and graphene oxide in different proportions of embodiment 3

[0065] In this example, in order to explore the antibacterial effect of coatings prepared by different proportions of composite antibacterial peptides and graphene oxide, the optimal concentration ratio of composite antibacterial peptides and graphene oxide was found out, and the composite antibacterial peptides and reduced Graphene oxide volume ratio is 1:1, 1:2, 1:2.5, the concentration of 1:3, the method for embodiment 2 prepares the aluminum alloy material GO-CT-1-A of corresponding coating, GO- CT-1-B, GO-CT-1-C, GO-CT-1-D, the experimental setting control group is aluminum alloy material without coating, as shown in Table 2 Table 2: Different antimicrobial peptides and Coating Preparation of Graphene Proportion

[0066]

[0067] 3.1 Determination of antibacterial properties

[0068] The antibacteri...

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Abstract

The invention provides a mechanical perfusion system. The mechanical perfusion system is characterized by comprising coatings prepared from graphene oxide and antimicrobial peptide; particularly, theperfusion system comprises an organ perfusate storage device and an organ storage device, the organ perfusate storage device and the organ storage device comprise graphene oxide and antimicrobial peptide, the graphene oxide and the antimicrobial peptide are prepared into the coating to coat the inner walls of the organ perfusate storage device and the organ storage device. The mechanical perfusionsystem can alleviate the postoperative infection phenomenon of organ transplantation, provide a stable internal environment for the organs and have good market prospects and high social public welfare value.

Description

technical field [0001] The invention belongs to the technical field of medical device design and manufacture, and in particular relates to a mechanical perfusion system capable of suppressing infection after transplantation. Background technique [0002] Organ transplantation is currently the most effective means of treating end-stage organ failure. Due to the continuous development of immunosuppressants, the improvement of organ transplant preservation technology and the good matching between recipients and donors, the quality of life of transplant patients has been significantly improved. With the clinical application of a large number of new high-efficiency immunosuppressants, the incidence of rejection is decreasing year by year. With the continuous improvement of normal temperature perfusion system and the continuous development of perfusate technology, the warm ischemia condition in the process of organ transfer and transportation has been improved, so that the compli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0247
Inventor 李建辉杨子江郑俊辛立民
Owner 嘉兴莱普晟医疗科技有限公司
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