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Multi-functional growth-promoting bacteria preparation special for soybean and preparation method and application thereof

A growth-promoting bacterial agent and multi-functional technology, applied in the field of microorganisms, can solve the problems that the long-term survival of the bacterial agent cannot be guaranteed, and it is difficult to meet the requirements of various nutrient elements of plants, so as to improve soybean yield, increase potassium content, and improve soil. effects of the environment

Active Publication Date: 2019-04-19
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ordinary microbial fertilizers such as phosphorus-dissolving bacteria or potassium-dissolving bacteria can only provide plants with a single nutrient element, which is difficult to meet the requirements of plants for multiple nutrients, and cannot guarantee that the bacteria agent can survive in the plant rhizosphere for a long time and play a stable role

Method used

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  • Multi-functional growth-promoting bacteria preparation special for soybean and preparation method and application thereof
  • Multi-functional growth-promoting bacteria preparation special for soybean and preparation method and application thereof
  • Multi-functional growth-promoting bacteria preparation special for soybean and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The screening of embodiment 1 bacterial strain

[0034] The strain screening method is as follows:

[0035] Obtain young soybean roots grown for 28-32 days, and put them into -1 Nystatin in sterile saline, at 28°C, 100-200r·min -1 Shake culture for 1-2h, adopt the ten-fold gradient method, and make 10 -1 to 10 -5 Concentration gradient bacterial suspension, respectively take 100-200μL from the bacterial suspension of each dilution and spread it on the phosphate-solubilizing bacteria solid medium plate with a pH of 7.0-7.2. K 2 HPO 4 2.0g, ammonium sulfate 0.5g, NaCl 0.3g, KCl 0.3g, MgSO 4 ·7H 2 O 0.3g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 4H 2 O 0.03g, Ca 3 (PO 4 ) 2 10g, 15g agar, 1000mL distilled water. Cultivate at 27-29°C for 24-36 hours, pick colonies with phosphorus-dissolving circles, separate and purify to obtain a single colony of phosphorus-dissolving bacteria; the obtained phosphorus-dissolving bacteria strains are inoculated into potassium-dissolvin...

Embodiment 2

[0037] Determination of Phosphorus and Potassium Ability of Example 2 Bacterial Strains

[0038] A total of 20 growth-promoting bacterial strains with dual functions of dissolving phosphorus and potassium, which have specific affinity with soybean, were screened from the soybean rhizosphere by selective medium and lectin double screening method, and the phosphorus-dissolving and potassium-dissolving ability of these strains were determined. ability.

[0039] Prepare the bacterial suspension of each strain, insert the bacterial strain into the phosphate-dissolving medium containing tricalcium phosphate, calcium phytate and lecithin with the inoculum size of 2%, and the medium formula is glucose 10g, K 2 HPO 4 2.0g, ammonium sulfate 0.5g, NaCl 0.3g, KCl 0.3g, MgSO 4 ·7H 2 O 0.3g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 4H 2 O 0.03g, insoluble phosphorus 10g, distilled water 1000mL, pH 7.2, and at 28℃, 160r·min -1 Shaking culture 5d. The content of available phosphorus in the cult...

Embodiment 3

[0044] The identification of embodiment 3 bacterial strains

[0045] Combining morphological observation, physiological and biochemical tests and 16SrRNA gene sequence comparison analysis to identify the species of FK19 strain.

[0046] According to "Common Bacteria System Identification Manual" and "Bergey's Bacterial Identification Manual", the physiological and biochemical characteristics of FK19 strain were detected. The morphological tests include Gram staining; the physiological and biochemical tests include starch hydrolysis test, V-P test, contact enzyme test, glucose oxidative decomposition test, hydrogen sulfide test, gelatin liquefaction test, and methyl red test. The results are shown in Table 2.

[0047] Table 2 Some physiological and biochemical characteristics of the strains

[0048]

[0049] The 16S rRNA gene sequence of the FK19 strain was determined, similar sequences were searched in the NCBI database, compared, and combined with the structure of physiol...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to a multi-functional growth-promoting bacteria preparation special for soybean. The multi-functional growth-promoting bacteria preparation is prepared by fermentation of FK19 strains, and the FK19 strains are classified and named as bacillus megaterium and preserved in China Center for Type Culture Collectionon the 27th December, 2018, and the preservation number is CCTCC M 2018925. The multi-functional growth-promoting bacteria preparation has the advantages that the multi-functional growth-promoting bacteria preparation has an obvious promoting effect on the growth of the soybean, the effects of improving the phosphorus content and the potassium content of soybean leaves are obvious, and the yieldof the bean can be improved. The roots of the soybean can generate soybean agglutinin, and the agglutinin is carbohydrate-binding protein or glycoprotein of a non-immune source and can be combined with polysaccharose of bacteria. The multi-functional growth-promoting bacteria preparation has soybean agglutinin compatibility, the multi-functional growth-promoting bacteria preparation can be combined with the soybean agglutinin and absorbed on the roots of the soybean, the soybean is further helped to synthesize more isoflavone matter, and mediated strains are fixed to the roots of the soybean,so that the growth-promoting function of the preparation is achieved.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a special multifunctional growth-promoting bacterium agent for soybeans, a preparation method and application thereof. Background technique [0002] Soybean is one of the main economic crops in my country and has a wide planting rate in China. Phosphorus and potassium are of great significance to soybean material accumulation, yield and quality. my country has a large population and little cultivated land. Although the soil is rich in phosphorus and potassium, most of them exist in the form of insoluble phosphorus and potassium, which are difficult to be absorbed and utilized by soybeans. In order to improve the yield and quality of soybeans, a large amount of phosphorus and potassium fertilizers are often used in agriculture, but the utilization rate of soybeans for phosphorus and potassium fertilizers is too low, and they cannot absorb the nutrients given by...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/11
CPCC12N1/20C12N1/205C12R2001/11
Inventor 曹媛媛郭婷婷李婷黎权德何相怡邱丽娟王晓波陈春钱叶张程飞
Owner ANHUI AGRICULTURAL UNIVERSITY
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