Promoter sequence based on BnaCnng52950D gene, recombinant vector and application

A promoter and gene technology, applied in the fields of genetic engineering and molecular biology, can solve the problems of wasting energy, easily affecting the physiological metabolism of plants, adverse pleiotropic reactions, etc., and achieve the effect of increasing diversity
CN109679956AActive Publication Date: 2019-04-26SOUTHWEST UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SOUTHWEST UNIVERSITY
Publication Date
2019-04-26

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Abstract

The invention belongs to the field of gene engineering and molecular biology, and particularly relates to a promoter sequence of a cabbage type rape BnaCnng52950D gene, a recombinant vector built according to the promoter sequence and application. A promoter element included in the sequence is predicted through bioinformatic software, and a plant expression vector is built for performing qualitative detection on the promotor activity. The result shows that a cloned sequence can drive the expression of a GUS reporter gene in the bases of pod stalks and leaves. The acquisition of the promoter provides a powerful tool for the transgenic research of rapeseed, and lays a certain foundation for the research of the rapeseed and genetically close species thereof.
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Description

technical field

[0001] The invention belongs to the field of genetic engineering and molecular biology, and in particular relates to a promoter sequence of BnaCnng52950D gene of Brassica napus, a recombinant vector and a transgenic plant constructed therefrom. Background technique

[0002] As one of the four major oil crops in the world, Brassica napus is the only winter oil crop in the world, and it is also the main oil, feed and energy crop in my country. Its perennial planting area and total output rank first in the world. But rape is still facing problems such as low yield and oil content, weak resistance and unsuitable for mechanized operation.

[0003] The promoter is located at the 5' end of the upstream of the gene and plays a vital role in the regulation of gene expression. It can be recognized by RNA polymerase and bind to it, and accurately initiate the transcription of a specific DNA sequence. The regulation of transcription level is the most cost-effective and ...

Claims

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