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h based on electrochromic materials 2 s-activated probes and their biological applications

A technology of electrochromic materials and probes, applied in the direction of material excitation analysis, color/spectral characteristic measurement, wave energy or particle radiation treatment materials, etc., can solve the problems that have not been reported in the application, achieve fast response rate, high tissue penetration The effect of penetration depth and high activation multiple

Active Publication Date: 2021-10-26
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Electrochromic materials have the advantages of environmental friendliness and low biological toxicity, but their applications in the fields of biomolecular detection and molecular imaging have not been reported yet.

Method used

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  • h based on electrochromic materials <sub>2</sub> s-activated probes and their biological applications
  • h based on electrochromic materials <sub>2</sub> s-activated probes and their biological applications
  • h based on electrochromic materials <sub>2</sub> s-activated probes and their biological applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1. H based on electrochromic material 2 Construction of S-Activation Probes

[0040] 1. Synthesis of electrochromic materials.

[0041] Synthesize electrochromic material 1 according to the following route 2+ (EM 1 2+ ).

[0042]

[0043] 2. Encapsulated EM 1 2+ , to prepare nanoparticles 12+ -NPs.

[0044] In this example, in order to prepare 1 2+ -NPs, 0.43 mg 1 2+ (BF 4 - ) 2 and 10 mg of DSPE-PEG2000 were dissolved in 1 mL of tetrahydrofuran. Then inject this solution into 9 mL of secondary water rapidly during the sonication process, and continue to sonicate for 10 minutes. Next, tetrahydrofuran was removed at 65° C. by bubbling nitrogen. The obtained solution was ultrafiltered with a 10K ultrafiltration tube at 3500 rpm for 15 minutes to wash the sample, and the obtained solution was stored in a refrigerator at 4° C. until use. Depend on figure 2 As shown in (b), the nanoparticles 1 were successfully prepared 2+ -NPs; figure 2 Amon...

Embodiment 2

[0051] Embodiment 2. Probe 1 2+ -SNP830 on human serum H 2 S detection

[0052] This embodiment utilizes probe 1 2+ -SNP830 on human serum H 2 S was tested.

[0053] Specific method: using exogenous H 2 S was used as an internal standard for the determination of H in plasma 2 Concentration of S by adding 1 μL of ZnCl to 200 μL of human plasma 2 to remove H from plasma 2 S, then 1 μL of secondary water, 5, 10, 20, 30, 40 and 50 μM of HS were sequentially added to the other 6 groups of 200 μL of human plasma - , after that, add 1 2+ -SNP830 (final concentration is 28 μg / mL PCPDTBT, 48 μg / mL 1 2+ (BF 4 - ) 2 ), the treated sample was incubated at 37°C for 10 min, and the fluorescence spectrum of the solution was measured with a fluorometer, and the excitation wavelength was 650nm.

[0054] Figure 5 In, (a) and (b) both show probe 1 2+ -SNP830 can be detected by H in human serum 2 S activation, and can quantify H in human serum 2 The concentration of S was 12.7±3...

Embodiment 3

[0055] Example 3. Probe 1 2+ -SNP830 on live RAW264.7 intracellular H 2 S detection and in vivo imaging analysis

[0056] This embodiment utilizes probe 1 2+ -SNP830 on live RAW264.7 intracellular H 2 S was tested.

[0057] Specific method: RAW264.7 cells (~5×10 4 ) were inoculated in confocal culture dishes and cultured overnight. add 1 to the cell 2+ -SNP830 (28 / 48 μg / mL PCPDTBT / 1 2+ (BF 4 - ) 2 ), the probe was incubated at 37°C for 3 h, the medium was removed, the cells were washed once with cold PBS, NaHS (1 mM) was added for incubation for 1 h, and the cells were washed three times with cold PBS. In order to regulate the endogenous H 2 Production of S, added to RAW264.7 cells without any treatment L -Cys (0,0.1,0.2 and 0.5mM), then incubated for different times (0,0.5,1 and 2h). At the same time, in order to remove endogenous H 2 S, adding ZnCl to cells without any treatment 2 (0.3mM), the cells were incubated for 10min. In order to inhibit the activity o...

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Abstract

A H based electrochromic material 2 S-activated probes and their biological applications. The present invention utilizes electrochromic materials, adopts nano-precipitation technology, and combines tumor targeting technology to design a new type of H 2 The S-activated probe comprises an electrochromic material, a fluorescent dye, a tumor cell-specific targeting substance and an amphiphilic polymer. The probe can be applied in vivo H 2 S detection and in vivo imaging have the advantages of high sensitivity, high tissue penetration depth, high signal-to-noise ratio and real-time non-invasive. In addition, the present invention also uses photosensitizers as fluorescent dyes to obtain H based on electrochromic materials. 2 S-activated photosensitive probes, combined with targeting functions, can effectively enter tumor cells 2 After S activation, laser irradiation produces a better photodynamic therapy effect, which can inhibit tumor growth and regulate endogenous H 2 The content of S can realize the complete elimination of tumor.

Description

technical field [0001] The invention belongs to the fields of biomolecular detection, living body imaging and photodynamic therapy, and specifically relates to a H 2 S-activated probes and their biological applications. Background technique [0002] h 2 S is the third gas signaling molecule after NO and CO, which plays a vital role in many biological processes, such as neurotransmission, vasodilation and anti-inflammation, endogenous H 2 S can relax blood vessels and inhibit the proliferation of vascular smooth muscle cells. But exception H 2 S levels are closely related to many diseases, such as Alzheimer's disease, Down's syndrome, diabetes, cirrhosis, hepatitis and cancer. Therefore, how to detect H in organisms by a highly selective and sensitive method 2 The level of S has received increasing attention from modern biomedical researchers. [0003] H has been reported so far 2 S detection methods, such as methylene blue method, chromatographic method of methyl brom...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00A61K41/00A61P35/00G01N21/33G01N21/64
Inventor 叶德举吴鲁艳孙一丹颜润琦
Owner NANJING UNIV
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