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Method for extracting plant samples by combining deep-eutectic solvent with microwave and deep-eutectic solvent

A technology of low eutectic solvent and extraction method, which is applied in solid solvent extraction, preparation of test samples, chemical/physical/physical chemical process of applying energy, etc. Pollution and other problems, to achieve the effects of high extraction efficiency, reduced environmental pollution, and simple operation

Inactive Publication Date: 2019-06-14
NANJING NORMAL UNIV CHANGZHOU INST OF INNOVATION & DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Common extraction solvents are methanol, ethanol, acetone, acetonitrile, toluene and other organic solvents. The use of these organic solvents will not only cause environmental pollution, but also be toxic and harmful to the human body
At the same time, different extraction solvents have different solubility of the extract, which will naturally affect the extraction effect.

Method used

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  • Method for extracting plant samples by combining deep-eutectic solvent with microwave and deep-eutectic solvent
  • Method for extracting plant samples by combining deep-eutectic solvent with microwave and deep-eutectic solvent
  • Method for extracting plant samples by combining deep-eutectic solvent with microwave and deep-eutectic solvent

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Experimental program
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Embodiment 1

[0035] Such as figure 1 As shown, the present embodiment 1 provides a plant sample extraction method, comprising the following steps: step S1, prepare the extract, that is, a diluted solution of deep eutectic solvent (Deep eutectic solvent, abbreviated as DES); step S2, extract the plant The sample is added to the extract to form a suspension; step S3, subjecting the suspension to microwave extraction; and step S4, centrifuging, and taking the supernatant.

[0036] Optionally, the plant sample, such as but not limited to rhubarb, can be processed into powder, so that the anthraquinones, such as aloe-emodin (Aloe-emodin), emodin ( Emodin), chrysophanol (Chrysophanol), emodin methyl ether (Physcion), etc.

[0037] Optionally, the solid-to-liquid ratio of the suspension is 0.005-0.045 g / mL.

[0038] The plant sample extraction method in Example 1 uses a deep eutectic solvent as the extraction solvent, and microwave-assisted extraction as the extraction means to extract the acti...

Embodiment 2

[0050] On the basis of Example 1, this Example 2 also provides a deep eutectic solvent for plant sample extraction, said deep eutectic solvent comprising: a hydrogen bond acceptor and a hydrogen bond donor; and both The mole fraction ratio is 1:1-4. Specifically, the deep eutectic solvent can be used as an extraction solvent in plant sample extraction.

[0051] Optionally, the plant sample can be rhubarb.

[0052] Further, the hydrogen bond acceptor includes but not limited to choline chloride; the hydrogen bond donor includes but not limited to one or more of organic acid, amide, polyalcohol; and the organic acid such as but It is not limited to carboxylic acid, citric acid and the like.

[0053] Regarding the component content of the deep eutectic solvent and the specific implementation process, please refer to the relevant discussion of Example 1, and will not repeat them here.

Embodiment 3

[0055] (1) Prepare the extract. Mix choline chloride and amide with a molar ratio of 1:1, heat to 60-120°C, stir for 10-120min to a homogeneous phase, then dilute with water to contain 20% DES to prepare an extract.

[0056] (2) Weibo-assisted extraction. Take 0.05g of rhubarb powder and add 10ml of the extract prepared in (1) (that is, the solid-to-liquid ratio of the extract is 0.005g / mL), and place it in a microwave extraction reactor with a power of 80-600W, and extract for 10 minutes. Take the supernatant after centrifugation.

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Abstract

The invention belongs to the field of comprehensive utilization of traditional Chinese medicine resources and particularly relates to a method for extracting plant samples by combining a deep-eutecticsolvent with microwave and the deep-eutectic solvent. The method for extracting the plant samples comprises the following steps: preparing an extraction solution which is a dilute solution of the deep-eutectic solvent; adding the plant samples into the extraction solution to form a suspension solution; carrying out microwave extraction on the suspension solution; and centrifuging and taking a supernatant solution. According to the method for extracting the plant samples, the deep-eutectic solvent is used as an extraction solvent, a microwave-assisted extraction method is used as an extractionmeans, and active components in the plant samples are extracted; the extraction solvent does not need to be consumed in the extraction process; the environmental pollution is reduced; the method hasthe characteristics of simple operation, low time consumption, low cost and high extraction efficiency.

Description

technical field [0001] The invention relates to the field of comprehensive utilization of traditional Chinese medicine resources, in particular to a method for extracting plant samples with a deep eutectic solvent combined with microwaves and a deep eutectic solvent. Background technique [0002] In recent years, with the improvement of people's living standards, more and more attention has been paid to improving health and natural green products, and plant active ingredients have attracted more and more attention due to their safety, reliability and significant activity. Plants contain a variety of biologically active substances, For example, organic acids, volatile oils, alkaloids, sugars, etc. all have physiological activities such as anti-oxidation, elimination of free radicals, and anti-inflammation, which meet people's pursuit of improving their own immunity. Therefore, its active ingredients are more and more used in the fields of food, medicine and daily chemicals. ...

Claims

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Application Information

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IPC IPC(8): B01D11/02B01J19/12G01N1/40
Inventor 毕文韬王佳琴林军陈大勇杨小弟
Owner NANJING NORMAL UNIV CHANGZHOU INST OF INNOVATION & DEV
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