A kind of preparation method of subcutaneous filling hyaluronic acid gel

Abstract

The invention relates to a method for preparing a subcutaneous filling, in particular to a method for preparing a hyaluronic acid gel for subcutaneous filling, and belongs to the technical field of biological material preparation. In the preparation method of the present invention, the method of introducing aldehyde groups and hydrazide groups into the polymer chain is adopted to realize the effect of fine needle injection and rapid in-situ molding, and avoid the explosion caused by the high viscosity of the preformed hydrogel and the difficulty of fine needle injection. Needle issues and biosafety issues caused by small molecule crosslinker residues. Moreover, in the preparation method of the present invention, the process of modifying aldehyde sodium hyaluronate with maleoyl groups after freeze-drying is used to realize the grafting of maleoyl groups with a high degree of substitution on the molecular chain of hyaluronic acid, so that hyaluronic acid can be used in subsequent raw materials. In the process of in-situ molding, further polymerization can occur under the irradiation of ultraviolet light, which greatly improves the adhesive strength of the gel to the tissue at the injection site, and completely overcomes the problem of the gel filler moving and shifting under external force extrusion.

Description

technical field

[0001] The invention relates to a method for preparing a subcutaneous filling, in particular to a method for preparing a hyaluronic acid gel for subcutaneous filling, and belongs to the technical field of biological material preparation. Background technique

[0002] Hyaluronic acid is a natural, non-immunogenic glycosaminoglycan composed of D-glucuronic acid and N-acetyl-D-glucosamine disaccharide units linked by glycosidic bonds. Due to its high water retention capacity and unique viscoelasticity, hyaluronic acid is increasingly used in the field of medical aesthetics, especially as a dermal filler to enhance facial features and reduce wrinkles and folds.

[0003] However, hyaluronic acid without structural modification has a short retention time in the body, and it is easy to be completely degraded and absorbed within a few weeks under the action of enzymes in the body, which brings difficulties to long-term shaping. For this reason, hyaluronic acid needs...