Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Moisturizing face cream containing exopolysaccharides of tremella fuciformis spores

An extracellular polysaccharide and moisturizing surface technology, applied in the field of skin care products, can solve the problems of low polysaccharide content, high cost, low efficiency, etc., and achieve the effects of excellent moisturizing effect, good moisturizing effect and good antioxidative effect.

Active Publication Date: 2019-07-02
SOUTH CHINA AGRI UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, when polysaccharides are extracted from the fruiting body of Tremella fungus for utilization, on the one hand, it takes a long time to grow into the fruiting body of Tremella fungus; Useful polysaccharides are not only costly, time-consuming and laborious, but also extremely low in efficiency, which limits the application of Tremella fruiting body polysaccharides
Moreover, and there are few studies on tremella exopolysaccharides at present, there is a lot of room for exploration

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Moisturizing face cream containing exopolysaccharides of tremella fuciformis spores
  • Moisturizing face cream containing exopolysaccharides of tremella fuciformis spores
  • Moisturizing face cream containing exopolysaccharides of tremella fuciformis spores

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] The screening of embodiment 1 Tremella fungus spores

[0062] The best strains were screened from six tremella spores Tyc63, Y2, Y11, Tr01, Tr21 and Tr9901.

[0063] 1. Rejuvenation and cultivation of tremella spores

[0064] (1) Inoculate the strains stored in the -80°C refrigerator on the PDA solid medium for activation by streaking or spreading on a plate, and then culture them in a 25°C incubator at a constant temperature for 2-3 days;

[0065] (2) Select a single colony with a good shape on the medium, and inoculate a ring with an inoculation loop in the PDB medium in the ultra-clean workbench, 150r / min, 25°C shake flask culture for 3 days and produce to the logarithmic growth phase . Then take it out and place it in a refrigerator at 4°C for later use.

[0066] 2. Molecular identification of tremella spores

[0067] 1. Preparation of template DNA for PCR identification

[0068] (1) Take out the Tremella fungus spore liquid strain growing to the logarithmic ph...

Embodiment 2

[0100] Example 2 Screening of Tremella fungus spore strains with higher exopolysaccharide production and determination of fermentation cycle

[0101] 1. Screening of Tremella fungus spore strains with higher exopolysaccharide production

[0102] 1. Extraction and separation of Tremella spore exopolysaccharide

[0103] Embodiment 1 has determined that these four strains of Tyc63, Tr01, Tr21, and Tr9901 are Tremella spores, so screening is carried out with the objects of these four Tremella spores, and the specific experimental process is as follows:

[0104] Tremella spore fermentation broth→centrifugation→concentrate the supernatant, add 3V absolute ethanol to precipitate polysaccharides→precipitate→polysaccharide crude product→heat and dissolve in distilled water→centrifuge→supernatant→Sevage method to remove protein→centrifuge to take supernatant→3V ethanol Precipitation of polysaccharides→precipitation→freeze drying→refinement of polysaccharides

[0105] Steps:

[0106] ...

Embodiment 3

[0134] Example 3 Antioxidative Activity Analysis of Tremella Fungus Bacillus Exopolysaccharide

[0135] 1. The scavenging effect of tremella spore exopolysaccharide on hydroxyl free radicals

[0136] With reference to the experiment of people such as Yan Jun, measure (Yan Jun etc., 2005) contain 8.8mmol / L H in the reaction system 2 o 2 1mL, 9mmol / L FeSO 4 1mL, 9mmol / L salicylic acid-ethanol 1mL, polysaccharide solution 1mL with different concentrations, and finally add H 2 o 2 Start the reaction, react at 37°C for 0.5h, and measure the absorbance of each concentration at 510nm with distilled water as a reference. Considering the light absorption value of the polysaccharide itself, with 9mmol / L FeSO 4 1mL, 9mmol / L salicylic acid-1mL ethanol, 1mL polysaccharide solutions of different concentrations and 1mL distilled water were used as the background absorption value of the polysaccharide.

[0137] The formula for calculating the clearance rate is:

[0138]

[0139] I...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
molecular weightaaaaaaaaaa
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses a moisturizing face cream containing exopolysaccharides of tremella fuciformis spores. A preparation process of the exopolysaccharides of the tremella fuciformis spores includes the following steps: taking a liquid supernatant of a fermentation broth of the tremella fuciformis spores, and adding absolute ethyl alcohol for performing precipitation; and then adding water to the precipitation for dissolution, then taking the liquid supernatant and removing a protein by utilizing a Sevage method, obtaining the liquid supernatant by centrifugation, then adding ethanol for performing the precipitation, and subjecting the precipitation to freeze drying to obtain the exopolysaccharides of the tremella fuciformis spores. The moisturizing face cream utilizes the fermentationbroth of the tremella fuciformis spores as a raw material, and separates and extracts the exopolysaccharides having a moisturizing effect, purified exopolysaccharides have a good moisturizing effect,and even the moisturizing effect is superior to that of glycerol; the exopolysaccharides simultaneously have a good antioxidant effect, the exopolysaccharides can be used as a moisturizing active ingredient and an antioxidant active ingredient for preparing a skin care product; and a prepared skin care product has a good moisturizing effect, and also has a function of repairing cell damage causedby UVB radiation.

Description

technical field [0001] The invention relates to the technical field of skin care products, in particular to a moisturizing face cream containing tremella exopolysaccharide. Background technique [0002] Tremella polysaccharide is a kind of heteropolysaccharide extracted and purified from the fruiting body of Tremella fungus, submerged fermentation spore liquid, etc. According to its nature and source, it can be divided into neutral polysaccharides, acid polysaccharides, exopolysaccharides, cell wall polysaccharides and acid oligosaccharides. Five types of sugar can promote the body's immune function, inhibit the deterioration of tumors, reduce blood sugar and blood lipid levels, remove free radicals from the body and slow down the aging of the body, and at the same time protect the body from radiation damage. [0003] However, when polysaccharides are extracted from the fruiting body of Tremella fungus for utilization, on the one hand, it takes a long time to grow into the f...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/73A61K8/9728A61Q19/00A61Q19/08
CPCA61K8/73A61K2800/85A61Q19/00A61Q19/004A61Q19/08A61K8/9728
Inventor 林俊芳郑倩望王久莹郭丽琼
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com