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Polyethylene glycol modified selenoprotein-zinc sulfate nano-liposome preparation method and application

A technology of nano-liposome and polyethylene glycol, which is applied in the application, molding or processing of animal feed, and additional food elements, etc., can solve the problem of reducing the potency of other nutrients, increasing the toxicity of meat products, and reducing the feed intake of piglets and other issues to achieve large-scale production, improve bioavailability, and improve biocompatibility

Inactive Publication Date: 2019-07-05
YANSHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are many defects in the use of high doses of sodium selenite and zinc sulfate in feed at present. The negative effects include: the raw materials of sodium selenite and zinc sulfate used in feed often contain heavy metals, and a large amount of feeding increases the risk of meat products. Toxicity is also a safety hazard for the environment
The amount of additives currently used in feed can promote the growth of piglets in the short term, but long-term feeding will reduce the feed intake of piglets and reduce the potency of other nutrients, and its toxicity can also induce symptoms such as anemia in piglets

Method used

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  • Polyethylene glycol modified selenoprotein-zinc sulfate nano-liposome preparation method and application
  • Polyethylene glycol modified selenoprotein-zinc sulfate nano-liposome preparation method and application
  • Polyethylene glycol modified selenoprotein-zinc sulfate nano-liposome preparation method and application

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preparation example Construction

[0024] The present invention describes a preparation method of protein selenium-zinc sulfate nano liposome modified by polyethylene glycol, the steps are:

[0025] In the reverse micellar system with soybean lecithin and cholesterol as the active agent and absolute ethanol as the organic solvent; add the aqueous solution of protein selenium and zinc sulfate in proportion to complete the encapsulation of protein selenium and zinc sulfate by the active agent, and then evaporate Remove absolute ethanol, then add a large amount of polyethylene glycol again, and use ultrasonic dispersion in an ultrasonic instrument to complete the modification of liposomes by polyethylene glycol, and then separate the protein selenium-zinc sulfate nano-lipids modified by polyethylene glycol body.

[0026] In the embodiment of the present invention, it includes the following steps:

[0027] (1) Soybean lecithin and cholesterol are dissolved in absolute ethanol in proportion, followed by ultrasonic ...

Embodiment 1

[0043] Put 30mg of soybean lecithin (purchased in Shenyang Tianfeng Biological Co., Ltd.), 5mg of cholesterol (purchased in Tianjin Damao Chemical Instrument Supply Station) into a round-bottomed flask, add 3mL of absolute ethanol, and ultrasonically dissolve them sufficiently to make The solution was transparent without precipitation; weighed 3mg protein selenium (purchased from Henan Yongshun Chemical Co., Ltd.) and 5mg zinc sulfate (purchased from Tianjin Dingshengxin Chemical Co., Ltd.) mixture, dissolved in 1mL water, ultrasonically dissolved it fully, and then It is added to the above-mentioned absolute ethanol solution, and the system is always in an inert gas environment, and the ratio of the water phase to the lipid phase is maintained at 1:3 to obtain the maximum encapsulation efficiency. To form reverse micelles, the two-phase system is sonicated in a bath sonicator until the mixture becomes a clear single-phase dispersion or an opalescent homogeneous dispersion. Us...

Embodiment 2

[0047] Put 40mg of soybean lecithin (purchased in Shenyang Tianfeng Biological Co., Ltd.), 8mg of cholesterol (purchased in Tianjin Damao Chemical Instrument Supply Station) into a round-bottomed flask, add 8mL of absolute ethanol, and ultrasonically dissolve them sufficiently to make The solution is transparent without precipitation; weigh 4mg of protein selenium (purchased from Henan Yongshun Chemical Co., Ltd.) and 4mg of zinc sulfate (purchased from Tianjin Dingshengxin Chemical Co., Ltd.) mixture, dissolve in 2mL of water, ultrasonically dissolve it fully, and then It is added to the above-mentioned absolute ethanol solution, and the system is always in an inert gas environment. Pay attention to maintaining the ratio of water to lipid phase at 1:4 to obtain the maximum encapsulation efficiency. To form reverse micelles, the two-phase system is sonicated in a bath sonicator until the mixture becomes a clear single-phase dispersion or an opalescent homogeneous dispersion. U...

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Abstract

The invention relates to the field of feed additives and discloses a polyethylene glycol modified selenoprotein-zinc sulfate nano-liposome preparation method and application. The method includes: in areverse micelle system with soybean lecithin and cholesterol serving as an active agent and absolute ethyl alcohol serving as an organic solvent, proportionally adding selenoprotein and zinc sulfateaqueous solution to complete coating of selenoprotein and zinc sulfate with an active agent; then, evaporating absolute ethyl alcohol, adding a great quantity of polyethylene glycol, ultrasonically dispersing in an ultrasonic apparatus to complete liposome modification with polyethylene glycol, and separating polyethylene glycol modified selenoprotein-zinc sulfate nano-liposome. The method is simple and easy in operation and avoids pollution; selenium and zinc element absorption of animals is increased, and biocompatibility and bioavailability of selenium and zinc elements in body circulationare further improved. The nano-liposome prepared according to the method is high in biocompatibility and encapsulation efficiency and less prone to phagocytosis by the spleen and the liver due to a particle size range of 100-200nm.

Description

technical field [0001] The invention relates to the field of feed additives, and relates to a preparation method and application of polyethylene glycol-modified protein selenium-zinc sulfate nano liposomes. Background technique [0002] Selenium and zinc are one of the essential trace elements for animals, which can promote the growth of piglets and reduce their diarrhea rate, and play an important role in the growth, reproduction, immunity, anti-oxidation and anti-inflammation of animals. Every cell, tissue, organ, gland and immune system in the growth process of animals needs some trace elements to maintain a normal metabolic balance, among which zinc and selenium are mainly absorbed from the feed and are crucial to the growth process. Lack of these elements can lead to metabolic abnormalities, poor growth and even disease. Since about 72% of my country's areas have low selenium or lack of selenium, and natural feed cannot provide enough zinc and selenium elements, supple...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K20/20A23K40/30A23K20/158
CPCA23K20/30A23K40/30A23K20/158
Inventor 高大威何雨初骆丽垚丛聪李磊张旭武
Owner YANSHAN UNIV
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