Signal-enhanced polymer functionalized rutile phase titanium dioxide mesocrystal based zearalenone detection method

A technology of zearalenone and rutile phase, which is applied in the direction of measuring devices, electrochemical variables of materials, instruments, etc., can solve the problems of large band gap and low photoelectric conversion efficiency, and achieve improved sensitivity and excellent biocompatibility and water-soluble effects

Inactive Publication Date: 2019-07-05
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, TiO 2 The band gap is large and can only be excited by ultraviolet lig

Method used

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  • Signal-enhanced polymer functionalized rutile phase titanium dioxide mesocrystal based zearalenone detection method
  • Signal-enhanced polymer functionalized rutile phase titanium dioxide mesocrystal based zearalenone detection method
  • Signal-enhanced polymer functionalized rutile phase titanium dioxide mesocrystal based zearalenone detection method

Examples

Experimental program
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Effect test

Embodiment 1

[0025] A photoelectrochemical immunoassay method for zearalenone based on signal-enhanced multimer functionalized rutile-phase titanium dioxide mesogens (e.g. figure 1 shown):

[0026] (1) Pretreatment of the glassy carbon electrode: the glassy carbon electrode is first mechanically polished and polished on the suede covered with alumina powder, washed with secondary water to remove the residual powder on the surface, and then moved into an ultrasonic water bath for cleaning until it is cleaned, and finally Wash thoroughly with ethanol, dilute acid and water;

[0027] (2) Dissolve 5 mg diphenylalanine (FF) powder in 1 mL hexafluoroisopropanol (HFIP) solution, then add 3 mg / mL mesoporous silica (MPS) aqueous solution dropwise to the above FF solution , the concentration ratio of the two was 4:1 to obtain a mixed solution. Then, 3 μL of the mixed solution was drip-coated on the surface of a clean glassy carbon electrode, dried in an oven, and cooled to room temperature;

[00...

Embodiment 2

[0033] Preparation of the mesoporous silica (MPS) material used in Example 1:

[0034] Synthesis of SiO by Hydrothermal Synthesis 2 Mesoporous materials, with tetraethyl orthosilicate (TEOS) as the source of silicon, triblock copolymer (P123, Chinese full name: polyethylene oxide-polypropylene oxide-polyethylene oxide tri-block copolymer ) and 1-carboxyethyl-3-methylimidazolium nitrate ionic liquid (PIL, purchased from Lanzhou Zhongke Kaitai Industry and Trade Co., Ltd.) as structural template units; first, 4g P123 and 2.67gPIL were completely dissolved in the secondary In water, use 2M HCl to control the pH of the solution; then, under constant stirring, slowly add 9gTEOS to the solution, stir at 40°C for 24h to obtain a gel-like substance; then, transfer it all to tetrafluoroethylene Aged titanium dioxide stainless steel autoclave for 48 hours at a temperature of 120°C, filtered the product, washed it with ethanol and secondary water, and dried it in an oven overnight; fin...

Embodiment 3

[0036] Envision@rTiO used in Example 1 2 MCs solution preparation:

[0037] 1) Rutile TiO 2 Mesoscopic crystals (rTiO 2 MCs) material preparation: 0.5 g sodium dodecylbenzenesulfonate (SDBS) was dissolved in 25 mL 2.2 mol / mL HCl solution, stirred for 15 minutes; then 0.5 mL titanium(IV) isopropoxide was added, and the Stirring at 48 h at C; subsequently, the obtained product was centrifuged, washed 4-5 times with ultrapure water and ethanol, and dried at 60°C for one day and night; finally, the above product was calcined at 400°C for 1h to remove residual Organic matter, the rutile TiO 2 Mesoscopic crystals (rTiO 2 MCs).

[0038] 2) Rutile TiO 2 Mesoscopic crystals (rTiO 2 MCs) labeled secondary antibody (Envision@rTiO 2 MCs) solution preparation: first, 5 mg of rTiO prepared in step 1) 2 MCs powder was uniformly dispersed with secondary water to obtain rTiO with a concentration of 5 mg / mL 2 MCs suspension, and adsorbed with 0.013M cysteamine aqueous solution a...

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Abstract

The invention discloses a signal-enhanced polymer functionalized rutile phase titanium dioxide mesocrystal based zearalenone detection method. Diphenylalanine (FF) can self-assemble to form dipeptide;the FF can be recombined with mesoporous silica (MPS) to form mesoporous silica nanospheres which is used for the construction of a sensing platform with a dipeptide compound; and a basal immobilizedantibody can be obtained through the covalent binding between the dipeptide and an antibody. A cysteamine-sensitized rutile phase titanium dioxide mesoscopic crystal (rTiO2 MCs) can significantly enhance photoelectrochemical response; Envision polymer containing a large number of antibodies can be recombined with the rTiO2 MCs to form an Envision@ rTiO2 MCs compound as a second antibody, and zearalenone (ZEN) can be identified through sandwich immunoreaction; and polymer functionalized rutile titanium dioxide mesocrystal can be taken as a photoelectrochemical probe, and a photoelectric signalcan be continuously enhanced with the continuous rising of zearalenone concentrations. Thus, high sensitivity detection on the zearalenone whose concentration is in a range of 10<-7>-10 ng/mL can berealized.

Description

technical field [0001] The invention belongs to the technical field of novel functional materials and biosensing detection, and specifically relates to a detection method for zearalenone based on a signal-enhanced multimer functionalized rutile phase titanium dioxide mesocrystal. Background technique [0002] Photoelectrochemical (PEC) detection uses light as the excitation signal and photocurrent as the detection signal. By using different forms of energy as the excitation signal and detection signal, the excitation and detection signals do not interfere with each other, so the background signal is low and can be obtain higher sensitivity. In the construction process of photoelectrochemical sensors, the choice of photosensitive materials is crucial to the response of the signal. Among the currently used materials, TiO 2 Nanomaterials are ideal materials for photocatalytic and photoelectrochemical sensors because of their unique photocatalytic activity, non-toxicity, and ex...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/327G01N27/416
CPCG01N27/305G01N27/308G01N27/327G01N27/416
Inventor 戴宏李佳宁任卉竹高利红张书培衣欢郑祥钦韩晴宋建榕
Owner FUJIAN NORMAL UNIV
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