Use of low ph active alpha-1,4/1,6-glycoside hydrolases as a feed additive for ruminants to enhance starch digestion

A ruminant, hydrolase technology, applied in the field of animal nutrition, can solve the problems of no raw starch binding domain, low efficiency, etc.

Pending Publication Date: 2019-07-05
DUPONT NUTRITION BIOSCIENCES APS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these feed enzymes do not appear to have a raw starch binding domain (SBD) and may have limited ability to hydrolyze non-gelatinized raw starch
[0019] Planchot et al (Extensive degradation of native starch granules by alpha-amylase from Aspergillus fumigatus [from Aspergillus fumigatus (Aspergillus fumigatus) alpha-amylase to the extensive degradation of native starch granules], Journal of Cereal Science [Journal of Cereal Science], vol. 21 Vol. 2, 1995, pp. 163-171) reported the inefficiency of alpha-amylases from Bacillus sp. in degrading granular starch compared with pancreatic amylase

Method used

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  • Use of low ph active alpha-1,4/1,6-glycoside hydrolases as a feed additive for ruminants to enhance starch digestion
  • Use of low ph active alpha-1,4/1,6-glycoside hydrolases as a feed additive for ruminants to enhance starch digestion
  • Use of low ph active alpha-1,4/1,6-glycoside hydrolases as a feed additive for ruminants to enhance starch digestion

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0293] Materials used in subsequent examples

[0294] Protein samples are listed in Table 1 and used in subsequent examples. Table 1 shows the enzyme type, the organism of origin of the samples (when known) and the internal or commercial source, and the patent reference for the sequence.

[0295] Table 1. List of enzyme components evaluated.

[0296]

example 2

[0298] Hydrolysis of corn flour by AkAA amylase in the presence of acid aspartic protease porcine pepsin and Trichoderma reesei AFP

[0299] The reaction mixture contained 1 mL of corn flour slurry (20%, w / w, pH 3.2), 20 μL of AkAA (209 mg / mL), 50 μL of porcine pepsin (Sigma, P7000, 10000 U / mL in water), 50 μL of Trichoderma reesei (5 mg / mL) of 5 μL and 50 μL of AFP and water to a final volume of 1.07 mL. The reaction was carried out at 40 °C for 5 h with shaking at 200 rpm. At the end of the reaction, 30 μL of the reaction supernatant was mixed with 0.23 mL of water and filtered through a 0.22 μm membrane filter. The filtrate (40 μL) was subjected to HPLC analysis on an Aminex HPX-87N HPLC column (Bio-Rad) at a flow rate of 0.6 mL / min at 78° C. using water as eluent for 15 min. Glucose and maltose peaks were detected using an in-line RI (refractive index) detector and peak areas were integrated using Chromeleon software (Dionex) according to the manufacturer's instructions....

example 3

[0305] Stability of AkAA amylase in the presence of rumen fluid

[0306] For an animal feed enzyme additive to be effective in ruminants, it is important that the enzyme have considerable stability in rumen fluid and rumen conditions. The data in Table 2 show that AkAA is stable when incubated at 40 °C for 4.5 h in the presence of cell-free rumen fluid, as the release of glucose and maltose was not significantly affected by increased rumen fluid volume.

[0307] The AkAA used had a total protein concentration of 209 mg / mL and rice starch granules (Sigma S7260) were prepared as an 8% (w / w) slurry in water with the pH adjusted to 3.2. After mixing with the pre-incubation mixture, the pH changed to pH 5.3. Cell-free rumen fluid had a pH of 6.1 collected from cows fed grass (41.66%), whole barley (14.79%), maize (16.78%), dried beet pulp and beet residue cake (3.02 %), Milled Barley (6.66%), Rapeseed Meal (4.88%), Soy Meal (4.46%), Komix (Vitamin and Mineral Mixture, 0.2%), Roed...

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Abstract

Disclosed are uses at least one alpha-1,4 / 1,6-glycoside hydrolase (GLCH) as a feed additive for a ruminant wherein said hydrolase: (a) has at least 20% activity at pH less than or equal to 3 in the presence of pepsin as compared to activity of the hydrolase at pH 6 in the presence of pepsin, (b) said hydrolase is active in at least two of three digestive chambers of a ruminant comprising a rumen,an abomasum and a small intestine and (c) the hydrolase works with pancreatic amylase to increase glucose yield.

Description

technical field [0001] The field relates to animal nutrition and in particular to low pH active alpha-1,4 / 1,6 glycoside hydrolases (GLCH) such as alpha-amylases, glucoamylases and alpha-glucosidases as feed for ruminants Use of an additive to enhance starch digestion. Background technique [0002] Ruminants have the unique ability to convert roughage into protein and energy through their microbial / enzymatic digestive system. Therefore, ruminants play an important role in the earth's ecology and food chain. [0003] The key difference between ruminants and non-ruminants is that the stomach of ruminants has four compartments: rumen, reticulum, omasum, and abomasum. In the first two chambers (rumen and reticulum), food mixes with saliva and separates into layers of solid and liquid matter. Solids pack together to form regurgitated food or boluses. [0004] The regurgitated food is then regurgitated and chewed to thoroughly mix it with saliva and break down the particle size...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K20/189A23K10/14A23K50/10
CPCA23K20/189A23K50/10A61P1/14A61K38/45A61K38/47A61K38/48A61K38/51A61K38/52
Inventor S·于K·M·克拉格W·李
Owner DUPONT NUTRITION BIOSCIENCES APS
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