SERS sensor of sandwich structure for detecting tyrosinase, and preparation and detection method thereof

Abstract

The invention discloses a SERS sensor of a sandwich structure for detecting tyrosinase, and a preparation and detection method thereof. The SERS sensor utilizes the specific recognition principle of the tyrosinase antigen antibody, and a solid phase antibody-to-be-detected antigen-labelled antibody sandwich structure is formed through the combination of the solid-phase antibody on a substrate andthe labelled antibody with the antigen through the adsorption of the magnetic substrate and the enhancement effect of the surface-enhanced Raman probe, the content of the tyrosinase is analyzed by recognizing a labelled molecular SERS signal, and then a surface-enhanced Raman analysis method for detecting the tyrosinase is established. The detection method disclosed by the invention has the advantages of being quick, sensitive, simple and less in sample consumption. Furthermore, the sensor for detecting the tyrosinase has good linearity, the detection limit is low, and can reach 1ng/mL. And meanwhile, the adding standard recovery rate of the tyrosinase in the human serum sample is in the range of 96.0%-136.0%. Therefore, the SERS sensor is expected to be an effective method for clinicallydetecting tyrosinase.

Description

technical field [0001] The invention belongs to the technical field of surface-enhanced Raman analysis and detection technology, and in particular relates to a SERS sensor with a sandwich structure for detecting tyrosinase and a preparation and detection method thereof. Background technique [0002] Tyrosinase (TYR) is a copper-containing oxidase that converts monophenols or catechols into the corresponding o-quinones, and is the rate-limiting enzyme in the biosynthesis of melanin from tyrosine. Tyrosinase is also a cytoplasmic melanocyte differentiation protein, which initiates the formation of melanin by mediating the oxidation of tyrosine to some quinones, and TYR is a potential biomarker for detecting melanoma, because its expression level correlates with malignant level closely related. In addition, abnormal levels of tyrosinase may lead to serious skin diseases such as vitiligo and neurological syndromes such as Parkinson's disease. Therefore, the determination of ty...

AI Technical Summary

Problems solved by technology

However, the detection sensitivity of these methods is not high enough, the specificity is low, and the chromatography and fluorescence analysis are limited by the requirements of tedious sample handling, complicated probe synthesis or expensive instruments.

Chinese Patent No. (CN 10285423313 B) discloses a tyrosinase biosensor based on modified electrodes and its preparation method, but this method is susceptible to environmental interference, and the detection reproducibility is poor

Chinese Patent No. (CN 109239255 A) discloses a method for assaying tyrosinase and its inhibitors based on the chitosan-platinum nanoparticle catalytic chromogenic system. The method detects tyrosinase through color changes. But the sensitivity is not high enough

Images