Method for detecting concentration of hydrogen peroxide
A hydrogen peroxide concentration and detection method technology, applied in the direction of measuring devices, material analysis through optical means, instruments, etc., can solve the problems of complex operation methods, damage to human health, long reaction time, etc., and achieve good fluorescence spectrum effects, Improved solubility and high sensitivity
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Embodiment 1
[0026] The invention provides a method for detecting hydrogen peroxide concentration, comprising:
[0027] Step 1, be 7.4 with pH, the Tris-HCl buffer solution that concentration is 0.05mol / L prepares carbon quantum dot solution, horseradish peroxidase solution, o-phenylenediamine solution and the hydrogen peroxide standard solution of different concentrations, wherein , the concentration of carbon quantum dot solution is 5.3×10 -6 g / mL, the concentration of horseradish peroxidase solution is 1×10 -5 g / mL, the concentration of o-phenylenediamine solution is 3×10 -2 mol / L; the preparation method of the hydrogen peroxide standard solution of different concentrations is: first prepare the hydrogen peroxide stock solution that concentration is 10mol / L with pure hydrogen peroxide and ultrapure water, carry out stock solution with described Tris-HCl buffer solution Gradient dilution, promptly, the concentration of the hydrogen peroxide standard solution of described different co...
Embodiment 2
[0032] A method for detecting the concentration of hydrogen peroxide, the process of which is roughly the same as in Example 1, the difference is that in step 2, first add 100 μl of horseradish peroxidase solution and 100 μl of adjacent The phenylenediamine solution was mixed and reacted at 37°C for 30 minutes, then 100 μl of carbon quantum dot solution was added respectively, stirred with a micro-injector, mixed and reacted for 1 minute at room temperature, and the standard sample system to be tested was obtained. In step 3, the standard sample to be tested is placed in the RF-6000 fluorescence spectrophotometer in sequence, the excitation wavelength is set to 380nm, the excitation and emission slits are both 5nm, and the standard sample to be tested is detected at 442nm and the fluorescence intensity at 573nm place, obtain the fluorescence spectrogram of described standard test sample system, as figure 1 As shown, among them, I442 is the fluorescence of carbon dots, I 573 i...
Embodiment 3
[0035] A method for detecting the concentration of hydrogen peroxide, the process of which is roughly the same as that of Example 1, the difference is that in step 1, when carbon quantum dots are prepared, pancreatin is dissolved in ultrapure water, and after dispersion, it is put into Put it into a magnetizer, magnetize it at a magnetic field strength of 12T for 15min, then place it at 37°C for 30min, then put it into an ultra-high pressure reaction tank, treat it at 240MPa for 5min, and finally put it into the reaction tank After reacting at 180°C for 10 h in the kettle, take it out and freeze-dry it.
[0036] The productive rate of the carbon quantum dot prepared in the present embodiment is 53.1%, adopting the method of the present embodiment to the detection limit of hydrogen peroxide is 1.52 * 10 -5 mol / L.
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