Method for determining short chain fatty acid content in intestinal contents or excrement

Abstract

The invention discloses a method for determining short chain fatty acid content in intestinal contents or excrement. A sample is pretreated by diethyl ether, and the short chain fatty acid content inthe sample is determined through a gas chromatographic method; and pretreatment temperature is 2-8 DEG C. In the method provided by the invention, other derivatization pretreatment steps are not needed, pretreatment time is less than 15min, length of determining time is less than 20min; low temperature can effectively reduce volatilization loss of the short chain fatty acid, and recovery rate is up to 98.10 + / -0.29%-101.30 + / -0.27%; with the method provided by the invention, limit of detection can be as low as 0.0036 <mu>L / mL, limit of quantitation can be as low as 0.0041 <mu>L / mL, so the method is more accurate and efficient for detection of the short chain fatty acid.

Description

technical field [0001] The invention relates to a method for measuring the content of short-chain fatty acids in intestinal contents or feces, belonging to the field of biological sample detection. Background technique [0002] The intestine is not only an important place for human digestion and absorption, but also the largest immune organ, which plays an extremely important role in maintaining normal immune defense functions. The human intestinal tract provides a good habitat for microorganisms. The microbial population living in the human gastrointestinal tract can reach tens of trillions, which is 10 times the number of human cells. More than 1000 species of bacteria have been identified and about 160 species of microorganisms are present in all individuals. The genome of intestinal microorganisms includes about 3 million genes, which is 150 times that of the human genome, and has metabolic functions that the human body does not possess. Intestinal microecology refers ...

AI Technical Summary

Problems solved by technology

First of all, the extraction efficiency of short-chain fatty acids in ultrapure water is not high, which will cause the detection value of the sample to be lower than the actual value; secondly, the effect of metaphosphoric acid in this method is to reduce the loss of short-chain fatty acids, but the treatment time of metaphosphoric acid is long (more than 2h) , which greatly reduces the experimental efficiency; finally, the sample contains a large amount of water, and direct injection will not only lead to a serious reduction in the life of the chromatographic column, but also seriously damage the detector.

Zhang Mengjie and others also used gas chromatography to quantify the content of short-chain fatty acids in feces. They also used dilute acid solution (2-ethylbutyric acid + hydrochloric acid) to extract short-chain fatty acids, and directly detected them in the gas phase through the membrane. This method also has shortcomings. The extraction rate of chain fatty acids is low (the recovery rate of standard addition is 83.75%~94.95%), the chromatographic analysis takes a long time (about 40min), and it is easy to damage the chromatographic column and instruments, etc.

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