Fermentation method for effectively improving content of soy isoflavone glycoside in soybean dreg feed

The technology of isoflavone aglycone and fermentation method is applied in the field of fermentation for effectively increasing the content of soybean isoflavone aglycone in bean dregs feed, which can solve the problems of harsh reaction conditions, incomplete fermentation, low conversion efficiency, etc. The method is simple and practical, and the effect is good

Inactive Publication Date: 2019-07-30
浙江康星生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Such as patents CN 1315323A and CN 1315322A, which adopt enzymatic hydrolysis of glycoside-type soybean isoflavone glycosides to produce aglycon-type soybean isoflavones, but they have disadvantages such as high cost, cumbersome process, and harsh reaction conditions; microbial fermentation is a relatively mild method. And an effective method, currently reported is the use of compound probiotics to ferment soybean meal feed to increase the conversion rate of soybean isoflavones. Although the fermentation process is relatively simple, the price of soybean meal is high, resulting in high fermentation costs; There are problems such as incomplete fermentation and low conversion efficiency

Method used

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  • Fermentation method for effectively improving content of soy isoflavone glycoside in soybean dreg feed

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Activation culture of Lactobacillus plantarum:

[0034] A, first configure the culture medium of plantarum lactobacillus;

[0035] Slope and plate medium: 5g yeast extract, 10g sodium chloride, 10g peptone, 20g agar, 15g glucose, 1L distilled water, natural pH. Sterilize at 110°C for 30 minutes. The composition of the liquid seed medium is the same as that of the slant medium, the difference is that no agar is added;

[0036] B, the activation culture method of Lactobacillus plantarum:

[0037] Culture method: under sterile conditions, inoculate Lactobacillus plantarum stored at 4°C into the slant medium, culture at 30°C for 30 hours to recover the strains, then draw colonies on the plate, pick the seeds and inoculate them into 100mL liquid medium respectively. In shake flask (250mL), 150r / min, 30 ℃ cultivated 24h, obtain plantarum lactobacillus fermented seed liquid, inoculate in a large amount of liquid culture medium with 1% inoculum size and expand cultivatio...

Embodiment 2

[0042] (1) The activation culture of plantarum lactobacillus, concrete operation is with embodiment 1;

[0043] (2) Configure the first-stage solid fermentation basal medium, and the weight percentages of each component are respectively: 96.6% of bean dregs; 1.3% of sucrose; 0.8% of cellobiase; 1.3% of glucoamylase, and mix uniformly to obtain a good Oxygen fermentation medium;

[0044] (3) Aspergillus niger powder is added to the one-phase aerobic fermentation medium, the inoculum size is 0.02% of the one-phase aerobic fermentation medium weight, the water content of the adjustment medium is 32%, and the fermentation temperature is 32°C, preferably Oxygen fermentation 4h, after carrying out the first-stage aerobic fermentation, obtain the first-stage fermentation mixture;

[0045] (4) the one-stage fermented mixture that the plantarum fermented seed liquid obtained in inoculation step (1) obtains in step (3), the inoculum size is 17% of the one-stage fermented mixture weight...

Embodiment 3

[0047] (1) The activation culture of plantarum lactobacillus, concrete operation is with embodiment 1;

[0048] (2) Configure the first-stage solid fermentation basal medium, and the weight percentages of each component are respectively: 99.9% of bean dregs; 0.04% of sucrose; 0.02% of cellobiase; Oxygen fermentation medium;

[0049] (3) Aspergillus niger powder is added to the one-phase aerobic fermentation medium, the inoculum size is 3% of the one-phase aerobic fermentation medium weight, the water content of the adjustment medium is 70%, and the fermentation temperature is 22 ℃, preferably Oxygen fermentation 4h, after carrying out the first-stage aerobic fermentation, obtain the first-stage fermentation mixture;

[0050] (4) the one-stage fermented mixture that the plantarum fermented seed liquid obtained in inoculation step (1) obtains in step (3), the inoculum size is 17% of the one-stage fermented mixture weight, and the total number of viable bacteria is 0.5 × 10 9 c...

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Abstract

The invention belongs to the field of biological feed, and relates to a fermentation method for effectively improving the content of soy isoflavone glycoside in soybean dreg feed. The method includesthe steps that lactobacillus plantarum is activated and cultured to obtain a lactobacillus plantarum fermentation seed solution; then a proper amount of cellobiase, glucose amylase and cane sugar areadded into soybean dregs and uniformly mixed to obtain a first-stage fermentation culture medium; aspergillus niger powder is added into the first-stage fermentation culture medium, the water contentand fermentation temperature are adjusted for first-stage bacterial enzyme collaborative fermentation, and a first-stage fermentation mixture is obtained; then the lactobacillus plantarum fermentationseed solution is inoculated in the first-stage fermentation mixture and uniformly mixed so as to obtain a second-stage fermentation mixture; second-stage anaerobic fermentation is carried out under asealing condition, and a finished product is obtained after fermentation is completed. Bacterial enzyme collaborative fermentation and aerobic and anaerobic step-by-step fermentation are used, the cheap soybean dreg raw materials are used, fermentation is thorough, the conversion degree of aglycone type soy isoflavone is high, the economic benefit is good, the palatability is good, and animals like to eat the feed.

Description

technical field [0001] The invention belongs to the field of biological feed, in particular to a fermentation method for effectively increasing the content of soybean isoflavone aglycone in bean dregs feed. Background technique [0002] Soybean isoflavones are a type of secondary metabolites formed during the growth of soybeans and other leguminous plants. Soybean isoflavones are currently known to have 12 isomers. Among them, 9 kinds exist in the form of glycosides, namely genistin, glycitin, daidzin, acetylgenistin, acetyldaidzin, acetyl soy bean Acetylglycitin, Malonylgenistin, Malonylglycitin and Malonyldaidzin. Only three exist in the form of aglycone, namely daidzein (Daidaein), genistein (genistein) and glycitein (Glycitein). The content of soybean isoflavones in soybeans is 97% to 98% in the form of glycosides, and the aglycon type soybean isoflavones only accounts for 2% to 3%. Soybean isoflavones have various physiological functions such as anti-oxidation, anti-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/37A23K10/14A23K20/163
CPCA23K10/12A23K10/37A23K10/14A23K20/163A23V2400/169Y02P60/87
Inventor 李继彬陈华友代爱校陈华撑倪忠
Owner 浙江康星生物科技有限公司
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