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Application of total flavone of wild jujube leaves

A technology of total flavonoids and wild jujube leaves, applied in the field of memory damage prevention and protection, total flavonoids of wild jujube leaves, can solve problems such as memory damage

Inactive Publication Date: 2019-07-30
SHANXI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The previous research of the research group showed that the flavonoids in jujube leaves mainly contain flavonols (quercetin, kaempferol) and flavanones (catechin, epicatechin) as aglycones, and have strong antioxidant activity , has a high ability to scavenge free radicals such as DPPH and ABTS, but there is no literature at present to mention that the total flavonoids of jujube leaves have the effect of anti-memory damage caused by brain aging

Method used

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  • Application of total flavone of wild jujube leaves
  • Application of total flavone of wild jujube leaves
  • Application of total flavone of wild jujube leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Preparation of total flavonoids from jujube leaves (ZSSLF)

[0018] Weigh 1000g of spiny jujube leaves, add 12L of 50% ethanol to soak for 3h, reflux extraction twice, each time for 0.5h, extract and filter, combine the filtrate, evaporate the solvent to get the solid spiny jujube leaf extract, apply D101 macroporous resin, and load the sample Concentration 0.5g·mL -1 , pass through the resin column at a flow rate of 0.5BV / h, wash away impurities with distilled water, then elute with 50% ethanol at a flow rate of 1BV / h, collect the eluate and evaporate the solvent to obtain 92g of Jujube leaf flavonoid extract powder. The purity of total flavonoids in jujube leaves was determined to be 40.95% by ultraviolet spectrophotometry;

Embodiment 2

[0020] Drug efficacy experiment, the effect on the memory ability of aging rats

[0021] experimental method:

[0022] (1) Model establishment and administration

[0023] Take 60 SPF grade healthy male SD rats, weighing 180-200g, provided by Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., divide them into cages according to 5 rats per cage, and feed them adaptively for 7 days, and then randomly Divided into 4 groups on average, 15 rats in each group, respectively blank control group Control, D-galactose model group Model, Suan jujube leaf total flavonoids low-dose group D-gal+ZSSLF (1g / kg), Suan jujube leaf total flavonoids high-dose group Group D-gal+ZSSLF (2g / kg). Except for the blank group, the rat aging model was prepared by intraperitoneal injection of D-galactose in the other 6 groups, that is, 300 mg / kg / bw D-galactose was injected intraperitoneally every day, and the blank group was replaced by normal saline for 36 consecutive days. At the same time a...

Embodiment 3

[0041] Drug efficacy experiment, the effect on biochemical indicators of aging rats

[0042] experimental method:

[0043]After the exploratory experiment, the rats were anesthetized by intraperitoneal injection of 10% chloral hydrate at a dose of 3.5 mL / kg, and blood was collected from the abdominal aorta into an EP tube. After resting, the whole blood was centrifuged at 3500r / min for 15min at 4°C, and the serum was collected and stored in a -80°C refrigerator for later use. The rats were dissected, the brain tissue was quickly taken out on ice and rinsed in ice saline, the blood was removed and dried with filter paper, after accurate weighing, 9 times the amount of ice saline was added, mechanically homogenized in an ice water bath, Finally, 10% brain tissue homogenate was obtained, and the obtained tissue homogenate was centrifuged at 3000r / min for 10min, and the tissue homogenate supernatant and serum were taken, and MDA, SOD, T-AOC, GSH, CAT content.

[0044] Statistic...

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Abstract

The invention relates to application of total flavone of wild jujube leaves and belongs to the technical field of medicine food. The aim is to provide novel application of the total flavone of the wild jujube leaves. The total flavone has a prevention and protection function on memory damage caused by brain senescence. According to the novel application of the total flavone of the wild jujube leaves, it is expressed through a Morris water maze experiment research result that through the total flavone of the wild jujube leaves, the learning and memory ability decline caused by aging can be obviously prevented, generation of MDA in serum and brain tissue of an aged mouse is effectively inhibited, the activity of SOD, T-AOC and CAT is enhanced, generation of a low-molecule free-radical scavenger GSH is promoted, and the total flavone has a prevention function on the brain aging and memory damage of the mouse caused by oxidative damage. In addition, the utilization rate of the side products wild jujube leaves in wild jujube seed production and processing is increased, and the problem is solved that since the demand quantity for wild resources of wild jujubes is increased, resources ofthe wild jujube leaves are wasted.

Description

technical field [0001] The invention belongs to the technical field of medicine and food, and specifically relates to the use of the total flavonoids of spinous jujube leaves, and the use of the total flavonoids of wild jujube leaves in the prevention and protection of memory damage caused by brain aging. Background technique [0002] With the continuous development of the aging trend of the world's population, anti-aging, a worldwide medical topic, has attracted increasing attention in China. In recent years, the research on aging and the search for efficient anti-aging drugs have become hot issues in the field of current medical research. Aging refers to a mature organism under normal conditions. With the increase of age, the internal environment stability and stress ability of the body decline, leading to gradual degenerative changes in structure and function, and an irreversible natural process. The central nervous system It is an important functional regulation system ...

Claims

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Application Information

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IPC IPC(8): A61K36/725A61P25/28A23L33/105A61K127/00
CPCA23V2002/00A61K36/725A23L33/105A61P25/28A23V2200/322
Inventor 闫艳付彩杜晨晖武宝爱杜会枝裴妙荣
Owner SHANXI UNIV
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